Transcription factor Ste12 regulates the spore maturation and proliferation of silkworm microsporidium Nosema bombycis

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

As the first identified microsporidium, Nosema bombycis can infect silkworm Bombyx mori to cause pebrine which brings huge economic losses sericulture all over world. The life cycle of is divided into three phases, including infective phase, proliferative phase and sporogonic phase. factor(s) that regulate spore maturation still unknown. Ste12 a fungi-specific transcription factor plays an important regulatory role in fungal development pathogenicity. purpose this study elucidate function homologous (NbSte12) bombycis. Here, we analyzed subcellular localization characteristics NbSte12. It was mainly expressed nucleus immature spores, NbSte12 had transcriptional activation activity, with its C-terminal necessary for ability. DNA affinity purification sequencing (DAP-seq) analysis revealed genes regulated by were involved maturation, cell division, energy metabolism. knockdown gene resulted decrease chitin layer thickness, germination activity proliferation. Then, electrophoretic mobility shift assay (EMSA) quantitative reverse polymerase chain reaction (RT-qPCR) analyses showed could bind promoters CHS7, SWP26, SWP30, PTP1 PTP2 genes, are wall polar tube formation, thereby positively regulating their transcription. indispensable formation layer, proliferation, thus microsporidium © 2025 Society Chemical Industry.

Language: Английский

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Establishment and application of a loop-mediated isothermal amplification method based on MetAP2 gene for the detection of Nosema bombycis in silkworms (Bombyx mori)

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 10, 2025

Pebrine, caused by Nosema bombycis , is a devastating disease of silkworms that causes huge economic losses to the sericulture farmers. Although pebrine quarantine disease, currently development effective molecular diagnostic or therapeutic tools for its management still lagging hotspot in research. In present study, highly specific, sensitive, and field-applicable LAMP assay targeting MetAp2 gene was developed. LM1 primer set produced better results, with fluorescence (amplification) signals appearing ~50 min. The reaction temperature 60.9°C outer inner ratio 1:8 were found be optimal, shortest amplification time strongest intensity. showed high specificity DNA spores, as templates other common microorganisms no amplification. detected pMD-19T-met positive plasmid at lowest concentration 10 3 copies, detection ~80 practicality test can detect spore −3 ng/μL. At 1.2 ng/μL, real-time appeared ~60 all life stages untreated silkworms. fumagillin treated silkworms, observed 90 h later, indicating reliability detecting effectiveness fumagillin, some degree, treating infection. developed holds good promise application specific tool detection/control field settings.

Language: Английский

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The unique transmembrane protein NbTMP2 (NBO_555g0004) of Nosema bombycis contributes to host infection

Journal of Invertebrate Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 108320 - 108320

Published: March 1, 2025

Language: Английский

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Construction of microsporidia‐inducible GAL4/UAS‐RTA system to generate resistance to Nosema bombycis in Bombyx mori

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 23, 2025

Abstract The presence of microsporidian infections in the animal industry could result substantial economic losses. Nosema bombycis , as first identified species microsporidia, poses a significant threat to silkworm industry. Currently, there is no strain with obvious resistance that can inhibit proliferation N. rearing. In this study, we developed microsporidia‐inducible GAL4/UAS‐RTA (Ricin toxin A chain) system silkworms confers against . This utilizes promoters BmUGT2 and BmUGT3 genes (P UGT2 P UGT3 ) drive expression GAL4 gene, while RTA driven by UAS cis ‐acting element. We generated hybrid through crosses between transgenic ‐ or silkworms. Under normal conditions, these lines exhibited unaltered fundamental characteristics compared wild‐type However, when exposed infection, they displayed significantly enhanced microsporidia. Our research successfully demonstrated mitigation microsporidia individuals using approach not only provides novel strategy for developing resistant strains but also serves an important reference genetically enhancing intracellular pathogens other economically insects.

Language: Английский

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Developing Pébrine-Resistant Silkworms Through Targeting the Transmembrane Protein Nbtmp1 in Nosema Bombycis

Published: Jan. 1, 2025

Language: Английский

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Nosema bombycis: A remarkable unicellular parasite infecting insects

Journal of Eukaryotic Microbiology, Journal Year: 2024, Volume and Issue: 71(5)

Published: Aug. 2, 2024

Abstract Microsporidia are opportunistic fungal‐like pathogens that cause microsporidiosis, which results in significant economic losses and threatens public health. Infection of domesticated silkworms by the microsporidium Nosema bombycis causes pébrine disease, for this species microsporidia has received much attention. Research been conducted extensively on over past few decades to better understand its infection, transmission, host–parasite interaction, detection. Several tools exist study including complete genome sequence N. . In addition understanding being important silkworm industry, become a model organism studying microsporidia. biology will contribute development knowledge regarding potential antimicrosporidia drugs. Furthermore, provide insight into molecular evolution functioning other fungal pathogens.

Language: Английский

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Strategies for diagnosing Nosema bombycis (Microsporidia: Nosematidae); the agent of pebrine disease

Molecular and Biochemical Parasitology, Journal Year: 2024, Volume and Issue: 260, P. 111645 - 111645

Published: June 20, 2024

Language: Английский

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Molecular characterization and phylogenetic analyses of MetAP2 gene and protein of Nosema bombycis isolated from Guangdong, China

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: June 28, 2024

Background Pebrine, caused by microsporidium Nosema bombycis , is a devastating disease that causes serious economic damages to the sericulture industry. Studies on development of therapeutic and diagnostic options for managing pebrine in silkworms are very limited. Methionine aminopeptidase type 2 ( MetAP2 ) microsporidia an essential gene their survival has been exploited as cellular target drugs such fumagillin its analogues several spp., including honeybees. Methods In present study, using molecular bioinformatics tools, we performed in-depth characterization phylogenetic analyses isolated from Guangdong province China. Results The full length sequence (Guangdong isolate) was found be 1278 base pairs (bp), open reading frame 1,077 bp, encoding total 358 amino acids. predicted presence typical alpha-helix structural elements, absence transmembrane domains signal peptides. Additionally, other characteristics stable protein were also predicted. homology-based 3D models with high accuracy reliability developed. expressed purified. observed weight ~43–45 kDa. showed acids sequences shared close evolutionary relationship spp. wild silkworms, but it divergent microsporidian insects, Aspergillus Saccharomyces cerevisiae higher animals humans. These indicated conservation relationships closely linked species relationships. Conclusion This study provides solid foundational information could helpful optimization treatment threat infection industry

Language: Английский

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Microsporidia secretory effectors and their roles in pathogenesis

Journal of Eukaryotic Microbiology, Journal Year: 2024, Volume and Issue: 71(5)

Published: Sept. 1, 2024

Abstract Microsporidia, a group of unicellular eukaryotic parasites, rely intensely on secretory effectors for successful invasion and proliferation within host cells. This review focuses the identification, characterization, functional roles effectors, including proteins microRNAs. The adhesion like Ricin‐B‐lectin facilitate initial invasion, which binds to cell surface. Once inside, microsporidia deploy range modulate immune responses, such as serpin proteins, redirect metabolism meet parasite's nutritional needs through hexokinase. Some microRNAs, alter gene expression create more favorable intracellular parasitic environment. In conclusion, play pivotal role spanning from establishment. future, secreted by will be studied, not only help elucidate molecular mechanism pathogenic manipulation but also provide potential targets anti‐parasitic treatments.

Language: Английский

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Transcriptomic analysis of Bombyx mori in its early larval stage (2nd instar) of development upon Nosema bombycis transovarial infection

Journal of Invertebrate Pathology, Journal Year: 2024, Volume and Issue: 206, P. 108157 - 108157

Published: June 21, 2024

Language: Английский

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