The Role of Dual Mutations G347E and E349D of the Pigeon Paramyxovirus Type 1 Hemagglutinin–Neuraminidase Protein In Vitro and In Vivo DOI Creative Commons
Yu Chen, Jiansen Gong, Tiansong Zhan

et al.

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(12), P. 592 - 592

Published: Nov. 25, 2024

Pigeon Newcastle disease (ND) is the most common viral infectious in pigeon industry, caused by paramyxovirus type 1 (PPMV-1), a variant of chicken-origin virus (NDV). Previous studies have identified significant amino acid differences between PPMV-1 and NDV at positions 347 349 hemagglutinin-neuraminidase (HN) protein, with predominantly exhibiting glycine (G) position glutamic (E) 349, while NDVs show E aspartic (D) 349. However, impact these substitutions remains unclear. In this study, we generated recombinant virus, NT-10-G347E/E349D, introducing G347E E349D dual mutations into strain NT-10 using reverse genetics. The biological characteristics NT-10-G347E/E349D were compared both vitro vivo. vitro, reduce NT-10's replication neuraminidase activity embryo fibroblast (PEF) cells enhancing chicken (CEF) cells. Additionally, decrease binding affinity to α-2,6 sialic receptor significantly increasing its for α-2,3 receptor. vivo, exhibited reduced pathogenicity pigeons but increased chickens parental strain. also pigeon-to-pigeon transmission enhanced from chickens. Notably, antigenic observed as an inactivated vaccine based on provided full protection against challenge immunized only 67% mortality NT-10-G347E/E349D. Overall, findings underscore critical role acids infection, pathogenicity, transmission, providing theoretical foundation scientific prevention control PPMV-1.

Language: Английский

Recent advancements in the diverse roles of polymerase-associated proteins in the replication and pathogenesis of Newcastle disease virus DOI Creative Commons
Jinghang Zhou, Yuqi Duan, Menglan Liu

et al.

Veterinary Research, Journal Year: 2025, Volume and Issue: 56(1)

Published: Jan. 12, 2025

Abstract Newcastle disease virus (NDV) is a significant member of the Paramyxoviridae family, known for causing epidemics and substantial economic losses in poultry industry worldwide. The NDV RNA genome primarily encodes six structural proteins (N, P, M, F, HN, L) two non-structural (V W). Among these, polymerase-associated viral (vRNA) form ribonucleoprotein complex, which plays crucial role synthesis transcription vRNA. In last decades, numerous studies have demonstrated that are linked to virulence, pathotype, thermostability NDV. Additionally, interactions between these host closely related NDV’s replication pathogenicity. Despite progress understanding unique shared functions proteins, research on proteins’ structure function less comprehensive than other available information often scattered. Therefore, this article systematically summarises reviews made features, correlation as well critical roles This review aims enhance our complex pathogenesis contribute development more effective vaccines antiviral drugs against challenges.

Language: Английский

Citations

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Chicken adipose tissue is differentially involved in primary and secondary regional immune response to NDV through miR-20a-5p-NR4A3 pathway DOI
Yi Jiang, Rui Zhang, Xinxin Xu

et al.

Veterinary Immunology and Immunopathology, Journal Year: 2025, Volume and Issue: 280, P. 110884 - 110884

Published: Jan. 13, 2025

Language: Английский

Citations

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Function of lamp2 Gene Response to Vibrio vulnificus Infection and LPS Stimulation in the Half-Smooth Tongue Sole (Cynoglossus semilaevis) DOI Open Access
Han Tian, Yufeng Liu, Mengchao Li

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1999 - 1999

Published: Feb. 25, 2025

Lysosome-associated membrane glycoproteins (LAMPs), including lysosomal protein 1 (Lamp1) and 2 (Lamp2), are involved in phagocytosis, chaperone-mediated autophagy (CMA), other pathways that interact with activity. However, the role of Lamp2 teleosts has not been clarified. In this study, we investigated functions lamp2 genes during Vibrio vulnificus infection. We achieved subcellular localization gene at cellular level performed overexpression RNA interference experiments followed by Lipopolysaccharides (LPS) stimulation to probe expression changes related genes. Ultrapathology analysis head-kidney revealed an increase lysosomes formation autophagosomal vesicles after V. infection, suggesting bind autophagosomes. The gene, encoding 401 amino acids Cynoglossus semilaevis, was constitutively expressed all examined tissues healthy half-smooth tongue sole, highest blood. A challenge test conducted assess response sole (Cynoglossus semilaevis) different concentrations vulnificus. results showed relative its genes-lc3, rab7, vamp8, atg14, stx17, snap29, ctsb, ctsd-varied time concentration gill, spleen, head-kidney, blood, liver, gut tissues. From experiments, it is hypothesized positively regulates lc3, negatively ctsd ctsb. Our findings provide new primary data for function sole., particularly regulating immune against

Language: Английский

Citations

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Understanding the silver nanotoxicity: mechanisms, risks, and mitigation strategies DOI
Muhammad Faran Akhtar, Muhammad Sultan Irshad, Shaukat Ali

et al.

Journal of Nanoparticle Research, Journal Year: 2025, Volume and Issue: 27(4)

Published: March 25, 2025

Language: Английский

Citations

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Oligoadenylate synthetase-like aggravated Newcastle disease virus–induced necroptosis in glioma cells DOI Creative Commons

Zecheng Yu,

Yuxin Chen, Sisi Chen

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: April 11, 2025

Newcastle disease virus (NDV) has emerged as a tumor-lysing agent in variety of cancers. Previous studies have shown that NDV cytolytic activity gliomas; however, the underlying mechanisms not been fully elucidated. Comparing glioma cells LN229 controlled group with infected rLa Sota-GFP strain, we strive to observe changes genome and protein levels well activation signalling pathways before after infection at cellular level genes transcriptome, study molecular mechanism necroptosis NDV-infected lethal LN229. We found which inhibited proliferation promoted apoptosis dose-dependent manner involved mitochondrial disruption by mechanism, whereas Fe2+ assay didn't change. Additionally, inhibitor Nec-1 alleviated damage caused during cells. Using RNA-seq analysis, pathway was significantly enriched cells, antiviral gene OASL (Oligoadenylate synthetase-like) up-regulated apoptotic pathway, could be directly induced infection. Knockdown attenuates infection-induced Our demonstrates on inducing necroptosis. targeting upregulation may provide novel strategy enhance necrotic

Language: Английский

Citations

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Macrophages are the target cells for genotype VII Newcastle disease virus and promote the infection and apoptosis of chicken splenic T cells DOI
Miao Cai, Qing Chen,

Yifei Liu

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 16, 2025

Abstract Infection with genotype VII Newcastle disease virus (NDV) poses a significant threat to poultry health, characterized by severe damage immune organs such as lymphocyte depletion. However, the precise mechanisms underlying this phenomenon remain unclear. Our present investigation focused on dynamic changes of macrophages, T cells, and B cells in spleen chickens infected NDV using multicolor flow cytometry. We found that selectively chicken splenic macrophages significantly increased number at 4 days post-infection. In contrast, became progressively depleted. vitro experiment revealed following infection, underwent apoptosis more potently when co-cultured than without macrophages. Overall, our findings highlight cell populations triggered illuminate role

Language: Английский

Citations

0
Explainable graph clustering via expanders in the massively parallel computation model DOI
Sepideh Aghamolaei,

Mohammad Ghodsi

Information Sciences, Journal Year: 2024, Volume and Issue: 677, P. 120897 - 120897

Published: June 7, 2024

Language: Английский

Citations

1
JEV infection leads to dysfunction of lysosome by downregulating the expression of LAMP1 and LAMP2 DOI

Xingmiao Yang,

Zheng Wang,

Shengda Xie

et al.

Veterinary Microbiology, Journal Year: 2024, Volume and Issue: 295, P. 110150 - 110150

Published: June 7, 2024

Language: Английский

Citations

1
Avian Coronavirus Infectious Bronchitis Virus Activates Mitochondria-Mediated Apoptosis Pathway and Affects Viral Replication by Inducing Reactive Oxygen Species Production in Chicken HD11 Cells DOI Creative Commons
Xiaoxiao Han,

Yuan Huang,

Junli Hao

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(7), P. 491 - 491

Published: July 1, 2024

Infectious bronchitis virus (IBV), a coronavirus that causes severe respiratory and gastrointestinal illness in poultry, leads to substantial economic losses. According earlier research, IBV infection chicken macrophage HD11 cells undergo cell apoptosis. Reactive oxygen species (ROS) the IBV-activated intrinsic apoptotic signaling pathway were examined this work. The findings demonstrate ROS accumulate. Moreover, decreased mitochondrial transmembrane potential cells, which could be blocked by antioxidants (PDTC NAC). two significantly affected expression of Bcl-2 Bax further inhibited activation caspase-3 apoptosis cells. Additionally, replication was blocking accumulation. Pretreating with ammonium chloride (NH

Language: Английский

Citations

1
HRS Facilitates Newcastle Disease Virus Replication in Tumor Cells by Promoting Viral Budding DOI Open Access
Yu Chen,

Chunxuan Wang,

Shunlin Hu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10060 - 10060

Published: Sept. 19, 2024

Newcastle disease virus (NDV) is a highly pathogenic avian infectious agent and also promising oncolytic with broad application prospects. The Endosomal Sorting Complex Required for Transport (ESCRT) machinery has been increasingly recognized its crucial role in the life cycles of enveloped viruses, influencing processes such as viral entry, replication, budding. In this study, we employed an RNA interference screening approach to identify key ESCRT components that regulate NDV replication tumor cells. qPCR, immunofluorescence, Western blot assays demonstrated knockdown HRS, CHMP4A, CHMP4B, CHMP4C significantly impaired HeLa cells, HRS exhibiting most pronounced inhibitory effect. Additionally, knockout inhibited budding suppressed NDV-induced cell death Notably, infection was shown upregulate gene protein expression time-dependent manner. conclusion, study systematically identifies critical involved within particular focus on promoting NDV's by budding, offering new insights development NDV-based therapies.

Language: Английский

Citations

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