Sodium hypochlorite inactivation of human CJD prions DOI Creative Commons
Bradley R. Groveman, Brent Race, Andrew G. Hughson

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(11), P. e0312837 - e0312837

Published: Nov. 7, 2024

Prion diseases are transmissible, fatal neurologic of mammals caused by the accumulation mis-folded, disease associated prion protein (PrPd). Creutzfeldt-Jakob Disease (CJD) is most common human and can occur sporadic onset (sCJD) (~85% CJD cases), genetic mutations in gene (10-15%) or iatrogenic transmission (rare). PrPd difficult to inactivate many methods reduce infectivity dangerous, caustic, expensive, impractical. Identifying viable safe for decontamination exposed materials critically important medical facilities research institutions. Previous has shown that concentrated sodium hypochlorite (bleach) was effective at inactivation prions derived from brains mice guinea pigs. Unfortunately, adapted rodents may mis-fold differently than humans, rodent not have same resistance susceptibility present bona fide prions. To confirm bleach efficacious against sourced prions, we different subtypes sCJD-infected brain homogenates concentrations increasing exposure times. Initial residual seeding activity following were measured using Real-Time Quaking Induced Conversion. In addition, tested how passage sCJD into either transgenic expressed protein, cerebral organoids (CO), two laboratory practices, affect prions' inactivation. Our results show both treatment time concentration factors brains, mouse CO all susceptible with as low a 10 percent solution 30-minute 50 little 1-minute time.

Language: Английский

Detection and decontamination of chronic wasting disease prions during venison processing DOI Open Access
Marissa S. Milstein,

Sarah C. Gresch,

Marc D. Schwabenlander

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Abstract Prion diseases, including chronic wasting disease (CWD), are caused by prions, which misfolded aggregates of normal cellular prion protein. Prions possess many characteristics that distinguish them from conventional pathogens, in particular, an extraordinary recalcitrance to inactivation and a propensity avidly bind surfaces. In mid late stages CWD, prions begin accumulating cervid muscle tissues. These features collectively create scenarios where occupational hazards arise for workers processing venison pose risks consumers through direct exposure via ingestion cross-contamination food products. this work, we show steel plastic surfaces used can be directly contaminated with CWD CWD-negative occur equipment had previously been CWD-positive venison. We also several decontaminant solutions (commercial bleach potassium peroxymonosulfate) efficacious on these same

Language: Английский

Citations

4
A same day α-synuclein RT-QuIC seed amplification assay for synucleinopathy biospecimens DOI Creative Commons
Sabiha Parveen, Parvez Alam, Christina D. Orrú

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: Feb. 11, 2025

Abstract Parkinson’s disease (PD), dementia with Lewy bodies (DLB), and other synucleinopathies are characterized by the accumulation of abnormal, self-propagating aggregates α-synuclein. RT-QuIC or seed amplification assays currently showing unprecedented diagnostic sensitivities specificities for even in prodromal phases years advance onset Parkinsonian signs dementia. However, commonly used α-synuclein take ≥48 h to perform as applied patients’ biospecimens. Here, we report development a faster assay that is analytically sensitive prior this type, but can be completed ≤12 brain, skin, intestinal mucosa, positive signals often arising <5 h. CSF took few hours longer. Our same-day (sdRT-QuIC) should increase practicality, cost-effectiveness, throughput measurements pathological forms fundamental research, clinical diagnosis, therapeutics development.

Language: Английский

Citations

0
Detection and Decontamination of Chronic Wasting Disease Prions during Venison Processing DOI
Marissa S. Milstein,

Sarah C. Gresch,

Marc D. Schwabenlander

et al.

Emerging infectious diseases, Journal Year: 2025, Volume and Issue: 31(4)

Published: March 18, 2025

Prion diseases, including chronic wasting disease (CWD), are caused by prions, which misfolded aggregates of normal cellular prion protein. Prions possess many characteristics that distinguish them from conventional pathogens, in particular, an extraordinary recalcitrance to inactivation and a propensity avidly bind surfaces. In middle late stages CWD, prions begin accumulating cervid muscle tissues. Those features collectively create scenarios occupational hazards arise for workers processing venison pose risks consumers through direct exposure ingestion cross-contamination food products. this study, we demonstrate steel plastic surfaces used can be directly contaminated with CWD CWD-negative occur equipment had previously been CWD-positive venison. We also show several decontaminant solutions (commercial bleach potassium peroxymonosulfate) efficacious on those same

Language: Английский

Citations

0
Rapid and sensitive determination of residual prion infectivity from prion-decontaminated surfaces DOI Creative Commons

Sara M. Simmons,

Vivianne L. Payne,

Jay G. Hrdlicka

et al.

mSphere, Journal Year: 2024, Volume and Issue: 9(9)

Published: Aug. 27, 2024

Prion diseases are untreatable fatal transmissible neurodegenerative that affect a wide range of mammals, including humans, and caused by PrP

Language: Английский

Citations

2
Sodium hypochlorite inactivation of human CJD prions DOI Creative Commons
Bradley R. Groveman, Brent Race, Andrew G. Hughson

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(11), P. e0312837 - e0312837

Published: Nov. 7, 2024

Prion diseases are transmissible, fatal neurologic of mammals caused by the accumulation mis-folded, disease associated prion protein (PrPd). Creutzfeldt-Jakob Disease (CJD) is most common human and can occur sporadic onset (sCJD) (~85% CJD cases), genetic mutations in gene (10-15%) or iatrogenic transmission (rare). PrPd difficult to inactivate many methods reduce infectivity dangerous, caustic, expensive, impractical. Identifying viable safe for decontamination exposed materials critically important medical facilities research institutions. Previous has shown that concentrated sodium hypochlorite (bleach) was effective at inactivation prions derived from brains mice guinea pigs. Unfortunately, adapted rodents may mis-fold differently than humans, rodent not have same resistance susceptibility present bona fide prions. To confirm bleach efficacious against sourced prions, we different subtypes sCJD-infected brain homogenates concentrations increasing exposure times. Initial residual seeding activity following were measured using Real-Time Quaking Induced Conversion. In addition, tested how passage sCJD into either transgenic expressed protein, cerebral organoids (CO), two laboratory practices, affect prions' inactivation. Our results show both treatment time concentration factors brains, mouse CO all susceptible with as low a 10 percent solution 30-minute 50 little 1-minute time.

Language: Английский

Citations

1