Genome-Wide Identification and Expression of Neuropeptides and Their Expression Patterns After RNAi of CHH Genes in Pacific White Shrimp Litopenaeus vannamei DOI Creative Commons
Long Zhang,

Lichao Sun,

Guanghao Song

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(12), P. 1038 - 1038

Published: Dec. 11, 2024

Neuropeptides are pivotal in regulating a broad spectrum of developmental, physiological, and behavioral processes throughout the life cycle crustaceans. In this comprehensive study, we utilized multiomics approach to characterize neuropeptide precursors assess expression profiles neuropeptide-encoding genes across various tissues developmental stages Pacific white shrimp, Litopenaeus vannamei. Additionally, explored differential eyestalk before after RNA interference-mediated suppression crustacean hyperglycemic hormone (CHH) vitellogenesis-inhibiting (VIH) gene expression. Our study identified total 125 L. vannamei, with 54 these previously uncharacterized genome. Notably, certain families showed significant expansion, as demonstrated by discovery 10 adipokinetic hormone/corazonin-like peptide (ACP) genes, 55 CHH superfamily 13 pigment-dispersing (PDH) genes. Alternative splicing was also found play crucial role generating functionally diverse neuropeptides; for example, agatoxin calcitonin undergo alternative that leads production three distinct neuropeptides two neuropeptides, respectively. Neuropeptide predominantly expressed neuroendocrine tissues, including eyestalk, cerebral ganglia, thoracic ventral ganglia. During embryonic development exception molt-inhibiting (MIH) gene, all monitored display minimal from zygote stage through larval membrane (Lim) stage. contrast, majority exhibit steady uptick nauplius onwards, culminating post-larval Furthermore, comparative transcriptomic analysis revealed downregulated following VIH This downregulation significantly associated enrichment pathways related amino acid metabolism synthesis. The findings provide valuable insights future research aimed at elucidating physiological functions potentially leading advancements shrimp aquaculture practices.

Language: Английский

Genome-Wide Identification and Expression of Neuropeptides and Their Expression Patterns After RNAi of CHH Genes in Pacific White Shrimp Litopenaeus vannamei DOI Creative Commons
Long Zhang,

Lichao Sun,

Guanghao Song

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(12), P. 1038 - 1038

Published: Dec. 11, 2024

Neuropeptides are pivotal in regulating a broad spectrum of developmental, physiological, and behavioral processes throughout the life cycle crustaceans. In this comprehensive study, we utilized multiomics approach to characterize neuropeptide precursors assess expression profiles neuropeptide-encoding genes across various tissues developmental stages Pacific white shrimp, Litopenaeus vannamei. Additionally, explored differential eyestalk before after RNA interference-mediated suppression crustacean hyperglycemic hormone (CHH) vitellogenesis-inhibiting (VIH) gene expression. Our study identified total 125 L. vannamei, with 54 these previously uncharacterized genome. Notably, certain families showed significant expansion, as demonstrated by discovery 10 adipokinetic hormone/corazonin-like peptide (ACP) genes, 55 CHH superfamily 13 pigment-dispersing (PDH) genes. Alternative splicing was also found play crucial role generating functionally diverse neuropeptides; for example, agatoxin calcitonin undergo alternative that leads production three distinct neuropeptides two neuropeptides, respectively. Neuropeptide predominantly expressed neuroendocrine tissues, including eyestalk, cerebral ganglia, thoracic ventral ganglia. During embryonic development exception molt-inhibiting (MIH) gene, all monitored display minimal from zygote stage through larval membrane (Lim) stage. contrast, majority exhibit steady uptick nauplius onwards, culminating post-larval Furthermore, comparative transcriptomic analysis revealed downregulated following VIH This downregulation significantly associated enrichment pathways related amino acid metabolism synthesis. The findings provide valuable insights future research aimed at elucidating physiological functions potentially leading advancements shrimp aquaculture practices.

Language: Английский

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