SARS-CoV-2 cellular coinfection is limited by superinfection exclusion
Journal of Virology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 21, 2025
ABSTRACT
The
coinfection
of
individual
cells
is
a
requirement
for
exchange
between
two
or
more
virus
genomes,
which
major
mechanism
driving
evolution.
Coinfection
restricted
by
known
as
superinfection
exclusion
(SIE),
prohibits
the
infection
previously
infected
cell
related
after
period
time.
SIE
regulates
many
different
viruses,
but
its
relevance
to
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
was
unknown.
In
this
study,
we
investigated
using
pair
SARS-CoV-2
variant
viruses
encoding
distinct
fluorescent
reporter
proteins.
We
show
first
time
that
limited
temporally
SIE.
defined
kinetics
onset
in
system,
showing
potential
starts
diminish
within
hour
primary
and
then
falls
exponentially
events
increased.
asked
how
these
would
affect
with
during
spreading
infection.
used
plaque
assays
model
localized
spread
observed
tissue
showed
restrict
coinfection—and
therefore
sites
possible
genetic
exchange—to
small
interface
viral
infections.
This
indicates
SIE,
reducing
likelihood
cells,
likely
reduces
opportunities
strains
an
underappreciated
factor
shaping
IMPORTANCE
Since
emerged
2019,
it
has
continued
evolve,
occasionally
generating
variants
concern.
One
ways
can
evolve
through
recombination,
where
information
swapped
genomes.
Recombination
requires
cells;
therefore,
factors
impacting
are
influence
phenomenon
whereby
becomes
increasingly
resistant
subsequent
Here
report
activated
following
Furthermore,
prevents
at
boundary
expanding
areas
infection,
scenario
most
lead
recombination
lineages.
Our
work
suggests
genomes
shapes
Language: Английский
A novel genotyping system based on site polymorphism on spike gene reveals the evolutionary pathway of porcine epidemic diarrhea virus
iMetaOmics.,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 6, 2025
Abstract
Porcine
epidemic
diarrhea
virus
(PEDV)
is
a
lethal
coronavirus
in
neonatal
piglets
characterized
by
rapid
evolution
both
genotype
and
phenotype.
However,
the
underlying
genetic
mechanism
has
not
been
completely
elucidated.
In
this
study,
we
investigated
PEDV
field
strains
circulating
China
between
2021
2022,
which
revealed
significant
divergence.
To
improve
classification
of
PEDV,
developed
site‐polymorphism‐based
genotyping
system
utilizing
global
sequences
from
public
databases.
While
there
are
currently
multiple
genotypic
classification‐based
systems
for
our
proposed
approach
could
offer
more
stable
considering
characteristics
evolutionary
dynamics.
Our
analysis
indicates
that
most
prevalent
lineages
originated
South
Korea
China,
with
G2c:
L4
predominating
L10
United
States.
Importantly,
discrete
phylogenetic
potential
pathways
showing
termini
S
gene
prone
to
recombination,
while
adaptive
selection
evident
middle
region.
Overall,
findings
provide
complementary
practical
methodology
novel
insights
into
pathway
coronaviruses.
Language: Английский