Developmental Medicine & Child Neurology,
Journal Year:
2023,
Volume and Issue:
65(8), P. 998 - 998
Published: April 27, 2023
Years
ago,
the
biomedical
community
convinced
itself
that,
once
we
knew
sequence
of
human
genome,
would
hold
keys
to
curable
universe.
Clearly,
this
is
far
from
case.
Unlocking
genome
made
us
realize
genetics
more
complex
than
ever
imagined.
To
be
fair,
gene-targeted
therapies
have
already
yielded
enormous
triumphs
and
promise
for
conquest
developmental
disorders
nervous
system.
But
they
also
many
challenges
demanded
different
solutions.1
Every
cell
in
body
has
same
complement
DNA
at
least
during
some
phase
its
lifetime.
a
pars
compacta
neuron
not
reticular
formation
astrocyte.
This
partly
because
represents
whole
repertoire
proteins
that
could
produced
by
cell,
but
which
ones
are
particular
cell.
Histone
methylation,
histone
acetylation,
transcription
factor
activation
binding,
presence
enhancers
suppressors
all
play
roles
gating
production
mRNA.
In
addition,
modification
status
individual
networks
genes
changes
over
time.2
For
reason,
understanding
impact
errors
or
modifications
requires
an
anatomical
temporo-physiological
map
each
these
biochemical
processes.
The
ability
do
single-cell
transcriptomics,
methylomic
acetylomic
analyses,
cryo-electron
microscopy
should
aid
basic
science
enterprise
translation
genetic
metabolic
disease
organ
organ,
system
system.3
Antisense
been
implemented
with
varying
degrees
success
several
neurological
diseases.
Challenges
include
need
cost
lifelong
therapy,
off-target
delivery,
adverse
effects
both
construct
delivery
vehicle
chemical
modification.
Furthermore,
variability
severity
course
makes
it
difficult
decide
whom
risks
therapy
outweigh
disease.4
It
critical
develop
standardized
metrics
progression
and,
them,
robust
natural
history
deep
phenotypic
subtypes
diverse
cohorts
patients.
We
must
use
prodigious
tools
understand
relationship
between
antisense
sequences
organs
target,
therapeutically
adversely.
Gene
most
often
delivered
viral
vehicles.
From
study
investigator
investigator,
viruses
vary
widely.
date,
there
no
road
virus
targets
tissue
type
causes
what
effects.
Virally-mediated
gene
known
cause
acute
hepatic
failure
microangiopathic
thrombocytopenia
renal
failure.
immune
reactions
mean
if
first
successful
correcting
disorder,
patient
likely
cannot
safely
effectively
receive
another.1
codify
best
specific
type.
effective,
non-toxic
methods
building
tolerance
given
virus.
explore
non-viral
systems,
such
as
nanoparticles,
avoid
presented
currently
available
strategies.
Because
answer
begets
another
question
fuller
reveals
unimaginable
complexity
biological
much
work
yet
done
effect
cures.
Only
through
innovative,
collaborative
efforts
will
achieve
goal
children
families
neurogenetic
disorders.5
Not
required
Molecules and Cells,
Journal Year:
2024,
Volume and Issue:
47(5), P. 100060 - 100060
Published: April 16, 2024
Transcriptome
analysis
is
widely
used
for
current
biological
research,
but
remains
challenging
many
experimental
scientists.
Here,
we
present
a
brief
broad
guideline
transcriptome
analysis,
focusing
on
RNA
sequencing,
by
providing
the
list
of
publicly
available
datasets,
tools,
and
R
packages
practical
analysis.
This
work
will
be
useful
biologists
to
perform
key
transcriptomic
with
minimum
expertise
in
bioinformatics.
Nutrients,
Journal Year:
2024,
Volume and Issue:
16(8), P. 1161 - 1161
Published: April 13, 2024
Colorectal
cancer
stands
as
the
third
most
prevalent
form
of
worldwide,
with
a
notable
increase
in
incidence
Western
countries,
mainly
attributable
to
unhealthy
dietary
habits
and
other
factors,
such
smoking
or
reduced
physical
activity.
Greater
consumption
vegetables
fruits
has
been
associated
lower
colorectal
cancer,
which
is
attributed
their
high
content
fiber
bioactive
compounds,
flavonoids.
In
this
study,
we
have
tested
flavonoids
quercetin,
luteolin,
xanthohumol
potential
antitumor
agents
an
animal
model
induced
by
azoxymethane
dodecyl
sodium
sulphate.
Forty
rats
were
divided
into
four
cohorts:
Cohort
1
(control
cohort),
2
(quercetin
3
(luteolin
4
(xanthohumol
cohort).
These
administered
intraperitoneally
evaluate
pharmaceutical
agents.
At
end
experiment,
after
euthanasia,
different
parameters
intestinal
microbiota
populations
analyzed.
Luteolin
was
effective
significantly
reducing
number
tumors
compared
control
cohort.
Furthermore,
main
significant
differences
at
level
observed
between
cohort
treated
experienced
reduction
abundance
genera
disease
inflammatory
conditions,
Clostridia
UCG-014
Turicibacter.
On
hand,
healthy
state,
Muribaculum,
showed
luteolin
results
underline
anti-colorectal
manifested
through
modulation
tumors.
BMC Ophthalmology,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: March 3, 2025
Pterygium,
abnormal
growths
of
conjunctival
tissue
onto
the
cornea,
are
common
ocular
surface
conditions
with
a
high
risk
recurrence
after
surgery
and
potential
ophthalmic
complications.
The
exact
cause
pterygium
remains
unclear,
triggers
still
unknown.
This
study
aims
to
investigate
relationship
between
epigenetics
uncover
identify
biomarkers
for
its
diagnosis.
We
performed
ChIP-seq
assay
compare
genome-wide
histone
modification
levels
normal
conjunctiva
stage
3
samples.
In
this
study,
we
epigenetic
profiles
patients
pterygium,
focusing
on
H3
lysine
4
(H3K4)
9
(H3K9)
trimethylation
(me3).
While
H3K4me3
showed
no
significant
change,
they
were
significantly
altered
in
genes
related
development
diseases.
Conversely,
H3K9me3
markedly
elevated
genome-wide,
particularly
at
promoters
82
involved
developmental
pathways.
Furthermore,
six
genes,
ANK2,
AOAH,
CBLN2,
CDH8,
CNTNAP4,
DPP6,
decreased
gene
expression
correlated
substantially
increased
H3K9me3,
suggesting
their
as
pterygium.
represents
first
report
linking
progression,
providing
valuable
insights
into
therapeutic
strategies
drug
targets.
Nucleic Acids Research,
Journal Year:
2025,
Volume and Issue:
53(7)
Published: March 23, 2025
In
yeast,
Hda1
histone
deacetylase
complex
(Hda1C)
plays
an
important
role
in
transcriptional
regulation
by
modulating
acetylation.
We
here
explored
the
changes
Hda1C
binding
nutrient-rich
and
-starved
conditions.
Chromatin
immunoprecipitation
sequencing
revealed
that
starvation
alters
RNA
Pol
II
to
coding
genes
a
highly
correlated
manner.
Interestingly,
we
discovered
transcription-independent
recruitment
of
intergenic
regions,
particularly
upstream
regulatory
sequences
(URS)
ribosomal
protein
(RP)
genes,
which
are
enriched
with
Rap1
sites.
Under
nutrient
starvation,
contributes
these
URS
where
deacetylates
histones,
thereby
fine-tuning
basal
gene
expression
delaying
RP
reactivation.
Furthermore,
is
also
required
for
I
transcription
RNAs
(rRNAs)
III
transfer
(tRNA)
especially
nutrient-limited
Significantly,
mutants
sensitive
translation
inhibitors
display
altered
ribosome
profiles.
Thus,
may
coordinate
within
nucleus
control
cytoplasm
could
be
key
regulator
responses
stress.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(15), P. 8362 - 8362
Published: July 31, 2024
Skeletal
muscle
atrophy,
characterized
by
diminished
strength
and
mass,
arises
from
various
causes,
including
malnutrition,
aging,
nerve
damage,
disease-related
secondary
atrophy.
Aging
markedly
escalates
the
prevalence
of
sarcopenia.
Concurrently,
incidence
atrophy
significantly
rises
among
patients
with
chronic
ailments
such
as
heart
failure,
diabetes,
obstructive
pulmonary
disease
(COPD).
Epigenetics
plays
a
pivotal
role
in
skeletal
elevates
methylation
levels
promoter
regions
specific
genes
within
tissues.
This
aberrant
is
similarly
observed
conditions
like
neurological
disorders,
cardiovascular
diseases.
study
aims
to
explore
relationship
between
epigenetics
thereby
enhancing
understanding
its
pathogenesis
uncovering
novel
therapeutic
strategies.
Molecules and Cells,
Journal Year:
2024,
Volume and Issue:
47(4), P. 100047 - 100047
Published: March 18, 2024
Aging
is
accompanied
by
the
gradual
deregulation
of
transcriptome.
However,
whether
age-dependent
changes
in
transcriptome
are
evolutionarily
conserved
or
diverged
remains
largely
unexplored.
Here,
we
performed
a
meta-analysis
examining
using
publicly
available
datasets
eleven
representative
metazoans,
ranging
from
Caenorhabditis
elegans
to
human.
To
identify
transcriptomic
associated
with
aging,
analyzed
various
aspects
transcriptome,
including
genome
composition,
RNA
processing,
and
functional
consequences.
The
use
introns
novel
splice
sites
tended
increase
age,
particularly
brain.
In
addition,
our
analysis
suggests
that
accumulation
premature
termination
codon-containing
transcripts
common
feature
aging
across
multiple
animal
species.
Using
C.
as
test
model,
showed
several
splicing
factors
age-dependently
downregulated
were
required
maintain
normal
lifespan.
Thus,
aberrant
processing
appears
be
short
lifespan
