KRASMutations in Cholangiocarcinoma: Prevalence, Prognostic Value, andKRASG12/G13 Detection in Cell-Free DNA DOI Open Access

PITCHASAK THONGYOO,

Jarin Chindaprasirt, Chaiwat Aphivatanasiri

et al.

Cancer Genomics & Proteomics, Journal Year: 2024, Volume and Issue: 22(1), P. 112 - 126

Published: Dec. 27, 2024

Background/Aim: Cholangiocarcinoma (CCA) is an aggressive hepatobiliary malignancy characterized by genomic heterogeneity. KRAS mutations play a significant role in influencing patient prognosis and guiding therapeutic decision-making. This study aimed to determine the prevalence prognostic significance of CCA, asses detection G12/G13 plasma cell-free DNA (cfDNA), evaluate value mutant allele frequency (MAF) cfDNA relation clinicopathological data survival. Materials Methods: A retrospective analysis 937 CCA patients was performed using from cBioPortal examine mutation profiles their association with Plasma 101 analyzed for droplet digital PCR, results were compared tissue-based sequencing 78 matched samples. Results: driver found 15.6% patients, common variants being G12D (37.0%), G12V (24.0%) Q61H (8.2%). Patients harboring exhibited decreased overall recurrence-free detected 14.9% samples, showing moderate concordance tissue sequencing, achieving 80% sensitivity 93% specificity. Elevated MAF cfDNA, combined high CA19-9 levels, correlated poorer survival outcomes. Conclusion: The presence associated poor underscoring importance as markers. demonstrated potential promising non-invasive alternative and, when may improve efficacy CCA.

Language: Английский

Establishment and genomic profiling of cholangiocarcinoma cells with functional characterization DOI Creative Commons

Rattanaporn Jaidee,

Apinya Jusakul, Piman Pocasap

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 13, 2025

Cholangiocarcinoma (CCA) is a highly lethal hepatobiliary malignancy, with prognosis influenced by anatomical subtypes and etiological factors. This study successfully established three CCA cell lines: KKU-097, KKU-466, KKU-610, from the primary tumors of patients in liver fluke-endemic areas. These cells represent perihilar (pCCA) intrahepatic (iCCA) subtypes. Comprehensive analyses, including histopathology, molecular profiling, biomarkers, cancer phenotype characterization, drug sensitivity testing standard chemotherapeutics, were conducted. Whole-exome sequencing was performed to explore genetic alterations. All lines exhibited adherent growth an epithelial morphology positive expression bile duct markers CK-7 CK-19. Cytogenetic analysis revealed complex hypertriploid karyotypes multiple chromosomal aberrations. Among lines, KKU-610 demonstrated higher invasion rates, whereas KKU-466 KKU-097 less aggressive phenotypes. Drug relative resistance gemcitabine as monotherapy combination cisplatin all cells. Genomic profiling identified targetable mutations, highlighting these new valuable models for investigating pathogenesis evaluating therapeutic strategies.

Language: Английский

Citations

0
A Machine Learning-Based Clustering Using Radiomics of F-18 Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography for the Prediction of Prognosis in Patients with Intrahepatic Cholangiocarcinoma DOI Creative Commons
Rosie Kwon, Hannah Kim, Keun Soo Ahn

et al.

Diagnostics, Journal Year: 2024, Volume and Issue: 14(19), P. 2245 - 2245

Published: Oct. 8, 2024

Intrahepatic cholangiocarcinoma (IHCC) is highly aggressive primary hepatic malignancy with an increasing incidence.

Language: Английский

Citations

1
Factors associated with prognosis and staging of intrahepatic cholangiocarcinoma DOI Open Access
Robert C. Chick, Samantha M. Ruff, Timothy M. Pawlik

et al.

Journal of Cancer Metastasis and Treatment, Journal Year: 2024, Volume and Issue: unknown

Published: July 10, 2024

Intrahepatic cholangiocarcinoma (ICC) is a relatively rare but aggressive primary liver cancer with poor prognosis. A number of established clinical and pathologic factors correlate prognosis, this reflected in the American Joint Committee on Cancer (AJCC) 8th Edition staging manual. Researchers have identified areas for improvement prognostication ICC using more nuanced tools, including serum biomarkers, molecular profiling, immunophenotyping, multimodal prognostic scoring systems. These data led to proposals novel systems that attempt improve correlation between stage More accurate tools may aid treatment decisions are tailored each individual patient, maximize therapy individuals most likely benefit avoid unnecessary toxicity decision regret those whom unlikely alter outcomes. Artificial intelligence machine learning help researchers develop new models predict outcomes accuracy precision.

Language: Английский

Citations

0
KRASMutations in Cholangiocarcinoma: Prevalence, Prognostic Value, andKRASG12/G13 Detection in Cell-Free DNA DOI Open Access

PITCHASAK THONGYOO,

Jarin Chindaprasirt, Chaiwat Aphivatanasiri

et al.

Cancer Genomics & Proteomics, Journal Year: 2024, Volume and Issue: 22(1), P. 112 - 126

Published: Dec. 27, 2024

Background/Aim: Cholangiocarcinoma (CCA) is an aggressive hepatobiliary malignancy characterized by genomic heterogeneity. KRAS mutations play a significant role in influencing patient prognosis and guiding therapeutic decision-making. This study aimed to determine the prevalence prognostic significance of CCA, asses detection G12/G13 plasma cell-free DNA (cfDNA), evaluate value mutant allele frequency (MAF) cfDNA relation clinicopathological data survival. Materials Methods: A retrospective analysis 937 CCA patients was performed using from cBioPortal examine mutation profiles their association with Plasma 101 analyzed for droplet digital PCR, results were compared tissue-based sequencing 78 matched samples. Results: driver found 15.6% patients, common variants being G12D (37.0%), G12V (24.0%) Q61H (8.2%). Patients harboring exhibited decreased overall recurrence-free detected 14.9% samples, showing moderate concordance tissue sequencing, achieving 80% sensitivity 93% specificity. Elevated MAF cfDNA, combined high CA19-9 levels, correlated poorer survival outcomes. Conclusion: The presence associated poor underscoring importance as markers. demonstrated potential promising non-invasive alternative and, when may improve efficacy CCA.

Language: Английский

Citations

0