Clinical Chemistry and Laboratory Medicine (CCLM),
Journal Year:
2023,
Volume and Issue:
62(1), P. 157 - 167
Published: July 28, 2023
Small
extracellular
vesicles
(EVs)
contain
various
signaling
molecules,
thus
playing
a
crucial
role
in
cell-to-cell
communication
and
emerging
as
promising
source
of
biomarkers.
However,
the
lack
standardized
procedures
impedes
their
translation
to
clinical
practice.
Thus,
we
compared
different
approaches
for
high-throughput
analysis
small
EVs
transcriptome.
Bioactive Materials,
Journal Year:
2024,
Volume and Issue:
36, P. 126 - 156
Published: March 2, 2024
Small
extracellular
vesicles
(sEVs)
are
known
to
be
secreted
by
a
vast
majority
of
cells.
These
sEVs,
specifically
exosomes,
induce
specific
cell-to-cell
interactions
and
can
activate
signaling
pathways
in
recipient
cells
through
fusion
or
interaction.
nanovesicles
possess
several
desirable
properties,
making
them
ideal
for
regenerative
medicine
nanomedicine
applications.
properties
include
exceptional
stability,
biocompatibility,
wide
biodistribution,
minimal
immunogenicity.
However,
the
practical
utilization
particularly
clinical
settings
at
large
scale,
is
hindered
expensive
procedures
required
their
isolation,
limited
circulation
lifetime,
suboptimal
targeting
capacity.
Despite
these
challenges,
sEVs
have
demonstrated
remarkable
ability
accommodate
various
cargoes
found
extensive
applications
biomedical
sciences.
To
overcome
limitations
broaden
potential
applications,
researchers
should
strive
deepen
understanding
current
loading,
characterization
techniques.
Additionally,
acquiring
fundamental
knowledge
about
origins
employing
state-of-the-art
methodologies
expand
research
scope.
This
review
provides
comprehensive
overview
exosome-based
strategies
diverse
domains,
encompassing
cancer
therapy,
immunotherapy,
biomarker
Furthermore,
we
emphasize
immense
exosomes
medicine.
Virology Journal,
Journal Year:
2024,
Volume and Issue:
21(1)
Published: Jan. 30, 2024
Abstract
Proteins,
RNA,
DNA,
lipids,
and
carbohydrates
are
only
some
of
the
molecular
components
found
in
exosomes
released
by
tumor
cells.
They
play
an
essential
role
healthy
diseased
cells
as
messengers
short-
long-distance
intercellular
communication.
However,
since
every
kind
cell
may
be
blood
other
bodily
fluids,
they
one
day
serve
biomarkers
for
a
wide
range
disorders.
In
many
pathological
conditions,
including
cancer,
inflammation,
infection,
role.
It
has
been
shown
that
biogenesis
is
analogous
to
viruses
exosomal
cargo
plays
propagation,
dissemination,
infection
several
viruses.
Bidirectional
modulation
immune
response
achieved
ability
associated
with
facilitate
immunological
escape
stimulate
body's
antiviral
response.
Recently,
have
received
lot
interest
due
their
potential
therapeutic
use
viral
infections
such
human
immunodeficiency
virus
(HIV),
Hepatitis
B
(HBV),
C
(HCV),
Epstein–Barr
(EBV),
SARS-CoV-2.
This
article
discusses
purification
procedures
detection
techniques
examines
research
on
biomarker
infection.
Graphical
abstract
Hematological Oncology,
Journal Year:
2025,
Volume and Issue:
43(1)
Published: Jan. 1, 2025
ABSTRACT
Multiple
myeloma
is
a
plasma
cell
malignancy
characterized
by
an
abnormal
increase
in
monoclonal
immunoglobulins.
Despite
significant
advances
treatment,
some
patients
progress
to
more
aggressive
forms
of
multiple
myeloma,
including
extramedullary
disease
or
leukemia.
Although
the
exact
molecular
mechanisms
are
not
known,
several
studies
have
confirmed
involvement
small
extracellular
vesicle‐enriched
microRNAs
progression.
Therefore,
we
performed
expression
profiling
these
molecules
bone
marrow
disease,
and
leukemia
using
RNA
sequencing
identify
novel
involved
pathogenesis.
In
total,
42
were
significantly
dysregulated
among
analyzed
subgroups.
Independent
validation
RT‐qPCR
elevated
levels
miR‐140‐3p,
miR‐584‐5p,
miR‐191‐5p,
miR‐143‐3p
compared
patients.
Subsequent
statistical
analysis
revealed
correlations
between
patient
clinical
characteristics
flow
cytometry
parameters
microRNA
expression.
These
results
indicate
that
dysregulation
could
contribute
Molecules,
Journal Year:
2024,
Volume and Issue:
29(11), P. 2417 - 2417
Published: May 21, 2024
Within
the
fields
of
infectious
disease
diagnostics,
microfluidic-based
integrated
technology
systems
have
become
a
vital
in
enhancing
rapidity,
accuracy,
and
portability
pathogen
detection.
These
synergize
microfluidic
techniques
with
advanced
molecular
biology
methods,
including
reverse
transcription
polymerase
chain
reaction
(RT-PCR),
loop-mediated
isothermal
amplification
(LAMP),
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR),
been
successfully
used
to
identify
diverse
array
pathogens,
COVID-19,
Ebola,
Zika,
dengue
fever.
This
review
outlines
advances
detection,
attributing
them
integration
traditional
methods
smartphone-
paper-based
diagnostic
assays.
The
cutting-edge
technologies
are
critical
importance
for
prevention
epidemic
surveillance.
Looking
ahead,
research
is
expected
focus
on
increasing
detection
sensitivity,
streamlining
testing
processes,
reducing
costs,
capability
remote
data
sharing.
improvements
aim
achieve
broader
coverage
quicker
response
mechanisms,
thereby
constructing
more
robust
defense
global
public
health
security.
Clinical Chemistry and Laboratory Medicine (CCLM),
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 19, 2024
Abstract
Extracellular
vesicles
(EVs)
represent
a
diverse
class
of
nanoscale
membrane
actively
released
by
cells.
These
EVs
can
be
further
subdivided
into
categories
like
exosomes
and
microvesicles,
based
on
their
origins,
sizes,
physical
attributes.
Significantly,
disease-derived
have
been
detected
in
virtually
all
types
body
fluids,
providing
comprehensive
molecular
profile
cellular
origins.
As
result,
are
emerging
as
valuable
addition
to
liquid
biopsy
techniques.
In
this
collective
statement,
the
authors
share
current
perspectives
EV-related
research
product
development,
with
shared
commitment
translating
newfound
knowledge
clinical
applications
for
cancer
other
diseases,
particularly
disease
biomarkers.
The
consensus
within
document
revolves
around
overarching
recognition
merits,
unresolved
questions,
existing
challenges
surrounding
EVs.
This
manuscript
is
collaborative
effort
led
Committee
Exosomes,
Society
Tumor
Markers,
Chinese
anti-Cancer
Association,
aimed
at
expediting
cultivation
robust
scientific
clinically
applicable
breakthroughs
propelling
field
forward
greater
swiftness
efficacy.
Drug Delivery and Translational Research,
Journal Year:
2023,
Volume and Issue:
13(12), P. 3154 - 3168
Published: June 26, 2023
Despite
the
efforts
and
advances
done
in
last
few
decades,
cancer
still
remains
one
of
main
leading
causes
death
worldwide.
Nanomedicine
particular
extracellular
vesicles
are
most
potent
tools
to
improve
effectiveness
anticancer
therapies.
In
these
attempts,
aim
this
work
is
realize
a
hybrid
nanosystem
through
fusion
between
M1
macrophages-derived
(EVs-M1)
thermoresponsive
liposomes,
order
obtain
drug
delivery
system
able
exploit
intrinsic
tumor
targeting
capability
immune
cells
reflected
on
EVs
thermoresponsiveness
synthetic
nanovesicles.
The
obtained
nanocarrier
has
been
physicochemically
characterized,
hybridization
process
validated
by
cytofluorimetric
analysis,
while
was
vitro
confirmed
use
fluorescent
probe.
Tumor
features
nanovesicles
were
vivo
investigated
melanoma-induced
mice
model
monitoring
accumulation
site
live
imaging
showing
higher
properties
compared
both
liposomes
native
EVs.
These
promising
results
ability
combine
advantages
nanotechnologies,
also
highlighting
their
potential
as
effective
safe
personalized
nanomedicine.
Current Issues in Molecular Biology,
Journal Year:
2024,
Volume and Issue:
46(11), P. 11957 - 11974
Published: Oct. 25, 2024
Lipedema
is
a
chronic
disorder
affecting
women
with
10%
incidence
worldwide.
It
often
confused
obesity.
This
study
was
undertaken
to
microRNAs
in
lipedema
tissue
assessed
by
direct
hybridization
using
the
robust
n-counter
flex
DX
CE-IVD
platform.
The
mean
age
of
subjects
participating
40.29
(±12.17).
body
weight
and
BMI
were
67.37
(±10.02)
25.75
(±4.10),
respectively.
stages
included
I
II.
differential
expressed
human
(hsa)-miRNAs
determined
according
log2
fold-change
(LFC)
0.5
Diagnostics,
Journal Year:
2025,
Volume and Issue:
15(2), P. 229 - 229
Published: Jan. 20, 2025
COVID-19
has
caused
widespread
morbidity
and
mortality,
with
its
effects
extending
to
multiple
organ
systems.
Despite
known
risk
factors
for
severe
disease,
including
advanced
age
underlying
comorbidities,
patient
outcomes
can
vary
significantly.
This
variability
complicates
efforts
predict
disease
progression
tailor
treatment
strategies.
While
diagnostic
therapeutic
approaches
are
still
under
debate,
RNA
sequencing
(RNAseq)
emerged
as
a
promising
tool
provide
deeper
insights
into
the
pathophysiology
of
guide
personalized
treatment.
A
comprehensive
literature
review
was
conducted
using
PubMed,
Scopus,
Web
Science,
Google
Scholar.
We
employed
Medical
Subject
Headings
(MeSH)
terms
relevant
keywords
identify
studies
that
explored
role
RNAseq
in
diagnostics,
prognostics,
therapeutics.
proven
instrumental
identifying
molecular
biomarkers
associated
severity
patients
COVID-19.
It
allows
differentiation
between
asymptomatic
symptomatic
individuals
sheds
light
on
immune
response
mechanisms
contribute
progression.
In
critically
ill
patients,
been
crucial
key
genes
may
outcomes,
guiding
decisions,
assessing
long-term
virus.
Additionally,
helped
understanding
persistence
viral
after
recovery,
offering
new
management
post-acute
sequelae,
long
COVID.
significantly
improves
management,
particularly
by
enhancing
accuracy,
personalizing
treatment,
predicting
responses.
refines
stratification,
improving
holds
promise
targeted
interventions
both
acute
