A delicate decision between repair and degradation of damaged lysosomes DOI Creative Commons
Yuchen Lei, Daniel J. Klionsky

Autophagy, Journal Year: 2024, Volume and Issue: 20(7), P. 1471 - 1472

Published: May 14, 2024

The destination of a damaged lysosome is either being repaired if the damage small or degraded through lysosome-specific macroautophagy/autophagy pathway named lysophagy when too extensive to repair. Even though previous studies report lumenal glycan exposure during as signal trigger lysophagy, it possibly beneficial for cells initiate earlier than membrane rupture. In recently published article, Gahlot et al. determined that SPART/SPG20 senses lipid-packing defects and recruits activates ubiquitin ligase ITCH, which labels lysosomes with chains lysophagy.

Language: Английский

ATG8ylation of vacuolar membrane protects plants against cell wall damage DOI Creative Commons
Jose Julian, Peng Gao, Alessia Del Chiaro

et al.

Nature Plants, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Abstract Vacuoles are essential for cellular metabolism and growth the maintenance of internal turgor pressure. They sequester lytic enzymes, ions secondary metabolites that, if leaked into cytosol, could lead to cell death. Despite their pivotal roles, quality control pathways that safeguard vacuolar integrity have remained elusive in plants. Here we describe a conserved pathway is activated upon wall damage turgor-pressure-dependent manner. Cell perturbations induce distinct modification—ATG8ylation—on membrane (tonoplast) regulated by V-ATPase ATG8 conjugation machinery. Genetic disruption tonoplast ATG8ylation impairs integrity, leading Together, our findings reveal homeostatic preserves damage.

Language: Английский

Citations

1
PRMT-7/PRMT7 activates HLH-30/TFEB to guard plasma membrane integrity compromised by bacterial pore-forming toxins DOI Creative Commons

Hui-Chen Hsieh,

I‐Hsiang Huang,

Shao-Wen Chang

et al.

Autophagy, Journal Year: 2024, Volume and Issue: 20(6), P. 1335 - 1358

Published: Jan. 23, 2024

Bacterial pore-forming toxins (PFTs) that disrupt host plasma membrane integrity (PMI) significantly contribute to the virulence of various pathogens. However, how cells protect PMI in response PFT perforation vivo remains obscure. Previously, we demonstrated HLH-30/TFEB-dependent intrinsic cellular defense (INCED) is elicited by maintain Caenorhabditis elegans intestinal epithelium. Yet, molecular mechanism for full activation HLH-30/TFEB elusive. Here, reveal PRMT-7 (protein arginine methyltransferase-7) indispensable nuclear transactivation HLH-30 PFTs. We demonstrate participates methylation on its RAG complex binding domain facilitate localization and activation. Moreover, showed PRMT7 evolutionarily conserved regulate TFEB repair damage caused PFTs human cells. Together, our observations not only unveil a novel PRMT-7/PRMT7-dependent post-translational regulation but also shed insight INCED against metazoans.

Language: Английский

Citations

4
Bioreceptor modified electrochemical biosensors for the detection of life threating pathogenic bacteria: a review DOI Creative Commons
Abdul Khaleque, Syed Imdadul Hossain, Md. Romzan Ali

et al.

RSC Advances, Journal Year: 2024, Volume and Issue: 14(39), P. 28487 - 28515

Published: Jan. 1, 2024

The lack of reliable and efficient techniques for early monitoring to stop long-term effects on human health is an increasing problem as the pathogenesis effect infectious bacteria growing continuously. Therefore, developing effective detection technique coupled with continuous pathogenic increasingly becoming a global public prime target. Electrochemical biosensors are among strategies that can be utilized accomplishing goal promising potential. In recent years, identifying target biological analytes by interacting bioreceptors modified electrodes most commonly used in electrochemical biosensing strategies. employed nucleic acid molecules (DNA or RNA), proteins, antibodies, enzymes, organisms, tissues, biomimetic components such molecularly imprinted polymers. Despite advancement biosensing, biosensor detecting still infancy stage so much room growth. A major milestone addressing some issues improving pathway investigation specific bacterial techniques. present study covers fundamental concepts biosensors, PB illnesses, latest based bioreceptor elements designed detect bacteria. This aims assist researchers up-to-date research work field bio-electrochemical monitoring.

Language: Английский

Citations

4
Lactiplantibacillus argentoratensis AGMB00912 alleviates salmonellosis and modulates gut microbiota in weaned piglets: a pilot study DOI Creative Commons
Ki‐Nam Yoon, Han Gyu Lee, Seo‐Joon Yeom

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 5, 2024

This study aimed to evaluate the efficacy of Lactiplantibacillus argentoratensis AGMB00912 (LA) in reducing Salmonella Typhimurium infection weaned piglets. The investigation focused on influence LA gut microbiota composition, growth performance, and fecal shedding. results indicated that supplementation significantly improved average daily gain reduced prevalence severity diarrhea. Fecal analysis revealed shedding LA-supplemented group. Furthermore, notably altered composition microbiota, increasing levels beneficial Bacillus decreasing those harmful Proteobacteria Spirochaetes. Histopathological examination showed less intestinal damage LA-treated piglets than controls. also observed affected metabolic functions related carbohydrate, amino acid, fatty acid metabolism, thereby enhancing health resilience against infection. Short-chain concentrations feces were higher group, suggesting microbial activity. enriched population bacteria, including Streptococcus, Clostridium, Bifidobacterium, while number such as Escherichia Campylobacter. These findings indicate potential a probiotic alternative for swine nutrition, offering protective effects

Language: Английский

Citations

4
A bioinspired microbial taste chip with artificial intelligence-enabled high selectivity and ultra-short response time DOI

Yining Wang,

Fengxiang Tang,

Boya Liu

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 277, P. 117264 - 117264

Published: Feb. 18, 2025

Language: Английский

Citations

0
Mycobacterium tuberculosis phagosome Ca 2+ leakage triggers multimembrane ATG8/LC3 lipidation to restrict damage in human macrophages DOI
Di Chen, Antony Fearns, Maximiliano G. Gutiérrez

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(13)

Published: March 26, 2025

The role of canonical autophagy in controlling Mycobacterium tuberculosis (Mtb), referred to as xenophagy, is understood involve targeting Mtb autophagosomes, which subsequently fuse with lysosomes for degradation. Here, we found that Ca 2+ leakage after phagosome damage human macrophages the signal triggers autophagy-related protein 8/microtubule-associated proteins 1A/1B light chain 3 (ATG8/LC3) lipidation. Unexpectedly, ATG8/LC3 lipidation did not target lysosomes, excluding xenophagy. Upon damage, leakage–dependent occurred on multiple membranes instead single or double noncanonical pathways. Mechanistically, from triggered recruitment V-ATPase–ATG16L1 complex independently FIP200, ATG13, and proton gradient disruption. Furthermore, limited restricted replication. Together, uncovered key mitigate damage.

Language: Английский

Citations

0
Lysosome-associated CASM: from upstream triggers to downstream effector mechanisms DOI Creative Commons
Namrita Kaur, Sven R. Carlsson, Alf Håkon Lystad

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: March 27, 2025

Lysosomes are dynamic organelles critical for cellular degradation and signaling, safeguarded by a limiting membrane that prevents leakage of harmful contents into the cytoplasm. Upon lysosomal damage, cells deploy defensive mechanisms, including key process called CASM (conjugation ATG8 to single membranes), which lipidates proteins onto support protective pathways. operates through two pathways: VAIL, induced pH changes via V-ATPase ATG16L1, STIL, triggered sphingomyelin exposure mediated TECPR1. This review examines CASM’s role in damage responses, exploring mechanisms damaging agents, distinctions between VAIL downstream effects decorating lysosomes with ATG8, effector recruitment repair or removal.

Language: Английский

Citations

0
Modular Design of Membrane-Impermeable Versatile Probe for Specific Imaging of Cell Walls and Real-Time Detection of Cell Membrane Damage DOI

Zhengdong Han,

Li Tian,

Ziqing He

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

The versatile fluorescent dyes are essential for specifically labeling plant cell walls in vivo, monitoring plasma membrane damage, and assessing viability. However, such rare often discovered accidentally due to a lack of design principles. Propidium iodide, well-known example, has limitations like low brightness, high toxicity, poor bacterial differentiation. To address these challenges, we developed VersaDye, modular probe designed specific imaging live damage cells, human certain bacteria. integrates impermeability principles environment-dependent fluorophore scaffolds. VersaDye enables bright, wash-free can stain various organs constructing 3D tissue organization. Notably, it selectively distinguish Gram-positive from Gram-negative bacteria, feature absent other dyes. Its targeting ability also allow caused by physical, chemical, biological stimuli. This study marks the first use analyzing plants under salt stress. offers robust platform wash-free, vivo simultaneous wall labeling. Additionally, its suggests adaptability regulating permeability meet diagnostic needs, as identifying membrane-compromised cells diseases or enabling high-throughput antibiotic screening

Language: Английский

Citations

0
Interplay of ferroptotic and apoptotic cell death and its modulation by BH3-mimetics DOI Creative Commons
Yun Qiu,

Juliana A. Hüther,

Bianca Wank

et al.

Cell Death and Differentiation, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

Language: Английский

Citations

0
The bacterial type three secretion system induces mechanoporation of vacuolar membranes DOI Creative Commons
Léa Swistak, Marvin Albert, Camila Valenzuela

et al.

PLoS Biology, Journal Year: 2025, Volume and Issue: 23(5), P. e3003135 - e3003135

Published: May 1, 2025

Endomembrane breaching is a crucial strategy employed by intracellular pathogens enclosed within vacuoles to access the nutrient-rich cytosol for replication. While bacteria use various mechanisms compromise host membranes, specific processes and factors involved are often unknown. Shigella flexneri, major human pathogen, accesses relying on Type Three Secretion System (T3SS) secreted effectors. Using in-cell correlative light electron microscopy, we tracked sequential steps of cell entry. Moreover, captured T3SS, which projects needle from bacterial surface, in process puncturing holes vacuolar membrane. This initial puncture ensures disruption vacuole. Together this introduces concept mechanoporation via secretion system as pathogen-induced membrane damage.

Language: Английский

Citations

0