Biological Chemistry, Journal Year: 2023, Volume and Issue: 405(1), P. 1 - 3
Published: Nov. 24, 2023
Language: Английский
Cells, Journal Year: 2024, Volume and Issue: 13(7), P. 607 - 607
Published: March 30, 2024
Membrane nanotubes (NTs) are dynamic communication channels connecting spatially separated cells even over long distances and promoting the transport of different cellular cargos. NTs also involved in intercellular spread pathogens deterioration some neurological disorders. Transport processes via may be controlled by cytoskeletal elements. frequently observed membrane projections numerous mammalian cell lines, including various immune cells, but their functional significance ‘antibody factory’ B is poorly elucidated. Here, we report that as active channels, B-lymphoma can mediate bidirectional mitochondrial transport, promoted cooperation two motor proteins, kinesin along microtubules myosin VI actin, supported heterogeneous arrangement main filament systems NTs. We revealed despite axons being extensions, they exhibit significant similarities. Furthermore, found improve stability lifespan B-lymphoma-cell NTs, while F-actin strengthens providing a structural framework for them. Our results contribute to better understanding regulation major humoral response infections.
Language: Английский
Citations
5
RSC Advances, Journal Year: 2024, Volume and Issue: 14(16), P. 11443 - 11451
Published: Jan. 1, 2024
A sequence of novel quinoline-8-yloxy and cinnamide hybrids has been synthesized evaluated for in vitro cytotoxicity against HepG2 liver cancer cells.
Language: Английский
Citations
5
Frontiers in Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 17
Published: April 3, 2024
Introduction Axonal plasticity is strongly related to neuronal development as well regeneration. It was recently demonstrated that active mechanical tension, intended an extrinsic factor, a valid contribution the modulation of axonal plasticity. Methods In previous publications, our team validated “nano-pulling” method used apply forces developing axons isolated primary neurons using magnetic nanoparticles (MNP) actuated by static fields. This found promote axon growth and synaptic maturation. Here, we explore use nano-pulling factor regeneration in tissue explant. Results Whole dorsal root ganglia (DRG) were thus dissected from mouse spinal cord, incubated with MNPs, then stretched. We particles able penetrate ganglion become localised both somas sprouting axons. Our results highlight doubles rate, this accompanied increase arborizing capacity axons, accumulation cellular organelles mass addition (endoplasmic reticulum mitochondria) pre-synaptic proteins respect spontaneous line on hippocampal neurons, observed process coupled density stable microtubules activation local translation. Discussion data demonstrate enhances whole exposed MNPs external These preliminary represent encouraging starting point for proposing biophysical tool design novel therapies based force promoting nerve
Language: Английский
Citations
4
Frontiers in Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 17
Published: June 7, 2024
Biological structures have evolved to very efficiently generate, transmit, and withstand mechanical forces. These biological examples inspired engineers for centuries led the development of critical insights concepts. However, progress in engineering also raises new questions about structures. The past decades seen increasing study failure engineered due repetitive loading, its origin processes such as materials fatigue. Repetitive loading is experienced by some neurons, example peripheral nervous system. This perspective, after briefly introducing concept fatigue, aims discuss potential effects based on our knowledge cellular responses stresses. A particular focus discussion are stress axons their cytoskeletal Furthermore, we highlight difficulty imaging these promise microscopy techniques. identification repair mechanisms paradigms underlying long-term stability an exciting emerging topic biology well a source inspiration engineers.
Language: Английский
Citations
3
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: May 11, 2025
Abstract Reconstructing the nigrostriatal pathway is one of major challenges in cell replacement therapies for Parkinson's disease due to lack enabling technologies capable guiding reinnervation dopaminergic precursors transplanted into substantia nigra toward striatum. This paper examines nano‐pulling, as a technology enable remote manipulation axonal growth. Specifically, an organotypic model consisting co‐cultures and striatum developed demonstrate that when cortical neural progenitors are nigra, nano‐pulling can guide enhance elongation projections To provide additional evidence, induced pluripotent stem cell‐derived progenitor neurospheres generated it shown induce guided growth promote maturation their processes. Altogether, this study demonstrates potential emerging technique directed within central nervous system.
Language: Английский
Citations
0
Published: Jan. 24, 2024
Membrane nanotubes (NTs) are dynamic communication channels connecting spatially separated cells even over long distances and promoting the transport of different cellular cargos. NTs also involved in intercellular spread pathogens deterioration some neuro-logical disorders. Transport processes via may be controlled by cytoskeletal elements. frequently observed membrane projections numerous mammalian cell lines including various immune cells, but their functional significance ‘antibody factory’ B is poorly elucidated. Here we report that as active channels, can mediate bidirectional mitochondrial transport, promoted cooperation two motor proteins, kinesin along microtubules myosin VI actin, supported heterogeneous arrangement main filament systems NTs. We revealed despite axons being extensions they exhibit significant similarities. Furthermore, found improve stability lifespan NTs, while F-actin strengthens providing a structural framework for them. Our results contribute to better understanding regulation major humoral response infections.
Language: Английский
Citations
1
Journal of Developmental Biology, Journal Year: 2024, Volume and Issue: 12(3), P. 25 - 25
Published: Sept. 17, 2024
Myofibers are highly specialized contractile cells of skeletal muscles, and dysregulation myofiber morphogenesis is emerging as a contributing cause myopathies structural birth defects. Myotubes the precursors undergo dramatic morphological transition into long bipolar myofibers that attached to tendons on two ends. Similar axon growth cones, myotube leading edges navigate toward target form cell-cell connections. The process guidance connects myotubes with correct tendons, orients morphology overall body plan, generates functional musculoskeletal system. Navigational signaling, addition mass volume, identification common events in guidance, but surprisingly, mechanisms regulating these not completely overlapping axons. This review summarizes strategies have evolved direct predetermined tendon highlights key differences between guidance. association pathways developmental disorders also discussed.
Language: Английский
Citations
0
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Journal Year: 2024, Volume and Issue: 1872(1), P. 119874 - 119874
Published: Nov. 7, 2024
Language: Английский
Citations
0
Computational and Structural Biotechnology Journal, Journal Year: 2024, Volume and Issue: 26, P. 78 - 91
Published: Nov. 29, 2024
Language: Английский
Citations
0
Biological Chemistry, Journal Year: 2023, Volume and Issue: 405(1), P. 1 - 3
Published: Nov. 24, 2023
Language: Английский
Citations
0