Hybrid RNA/DNA Concatemers and Self-Limited Complexes: Structure and Prospects for Therapeutic Applications DOI Creative Commons

Maria A. Kanarskaya,

С. В. Новикова, Alexander A. Lomzov

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(24), P. 5896 - 5896

Published: Dec. 13, 2024

The development of new convenient tools for the design multicomponent nucleic acid (NA) complexes is one challenges in biomedicine and NA nanotechnology. In this paper, we analyzed formation hybrid RNA/DNA concatemers self-limited by a pair oligonucleotides using UV melting, circular dichroism spectroscopy, gel shift assay. Effects size linker between duplex-forming segments on complexes’ shape number subunits were compared systematized RNA/DNA, DNA/DNA, RNA/RNA assemblies. data complex types summarized here as heat maps offer tool constructs. General rules found allow not only designing with desired structures but also purposefully transforming their geometry. A-form double helix studied was confirmed analysis. Moreover, show first time efficient degradation RNA RNase H imidazole. results open up prospects supramolecular nanotechnology, nanomachinery, biomedical applications.

Language: Английский

Integrating RNA Interference and Nanotechnology: A Transformative Approach in Plant Protection DOI Creative Commons
Mohammad Shafiqul Islam, Md Robel Ahmed, Muhammad Noman

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 977 - 977

Published: March 20, 2025

RNA interference (RNAi) has emerged as a potent mechanism for combating pathogenic fungi and oomycetes over the past decades. It offers promising gene-silencing approach by targeting crucial genes involved in diseases caused economically scientifically significant fungal pathogens, such Botrytis cinerea Fusarium species. Simultaneously, nano-agro-products have gained attention alternatives to traditional fungicides plant protection strategies. However, instability of naked molecules outside cellular environment presents challenge, they degrade rapidly, limiting their efficacy prolonged disease control. Concerns regarding toxicity protective nanoparticles non-target organisms also arisen. Integrating RNAi with nano-agro-products, particularly nanocarriers, form RNA-nano complexes demonstrated potential, providing enhanced stability, reduced toxicity, extended This review explores mechanisms complexes-mediated protection, addressing stability nano-toxicity issues while examining prospects complex research pathogen management.

Language: Английский

Citations

1
RNA-based therapies in liver metabolic diseases DOI Creative Commons
Antonio Fontanellas, Pedro Berraondo, F Urigo

et al.

Gut, Journal Year: 2025, Volume and Issue: unknown, P. gutjnl - 331742

Published: Feb. 23, 2025

RNA-based therapeutics have rapidly emerged over the past decade, offering a new class of medicines that differ significantly from conventional drugs. These therapies can be programmed to target or restore defective genes, allowing for more personalised treatments and reducing side effects. Notably, RNA made significant progress in treatment genetic liver diseases, exemplified by small interfering hereditary transthyretin amyloidosis, which use liver-targeting strategies such as GalNAc conjugation improve efficacy safety. gene-editing technologies, base editor prime clustered regularly interspaced short palindromic repeats systems, also show promise with their ability minimise genomic rearrangements cancer risk. While offer high precision, challenges remain optimising delivery methods ensuring long-term safety efficacy. Lipid nanoparticle-mRNA therapeutics, particularly protein replacement rare gained support preclinical successes. Compared viral gene therapies, mRNA present safer profile reduced risks integration oncogene activation. However, clinical trials, especially face limitations sample sizes observation periods. Further studies, including non-human primates, will essential refining trial designs. Despite potential, costs pose challenge require cost–utility models guide pricing accessibility. Here, we discuss fundamental aspects showcase most relevant developments metabolic diseases.

Language: Английский

Citations

0
Revolutionizing mRNA Vaccines Through Innovative Formulation and Delivery Strategies DOI Creative Commons
Munazza Fatima,

Timothy An,

Kee‐Jong Hong

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 359 - 359

Published: March 1, 2025

Modernization of existing methods for the delivery mRNA is vital in advanced therapeutics. Traditionally, has faced obstacles poor stability due to enzymatic degradation. This work examines cutting-edge formulation and emerging techniques safer vaccines. Inspired by success lipid nanoparticles (LNP) delivering vaccines COVID-19, a variety other formulations have been developed deliver diverse infections. The meritorious features nanoparticle-based strategies, including LNP, polymeric, dendrimers, polysaccharide-based, peptide-derived, carbon metal-based, DNA nanostructures, hybrid, extracellular vesicles, examined. impact these platforms on vaccine efficacy, protection from degradation, cellular uptake, controlled release, immunogenicity discussed detail. Even with significant developments, there are certain limitations overcome, toxicity concerns, limited information about immune pathways, need maintain cold chain, necessity optimizing administration methods. Continuous innovation essential improving systems Future research directions proposed address challenges expand their potential prophylactic therapeutic application.

Language: Английский

Citations

0
Plugging synthetic DNA nanoparticles into the central dogma of life DOI Creative Commons
Kayla Neyra,

Shirishkant Vrijray Desai,

Divita Mathur

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Recent advancements have engineered an approach to encode genetic sequences in DNA nanoparticles, making them a promising platform for gene therapy. This article highlights design considerations of these structures optimal protein expression.

Language: Английский

Citations

0
Hybrid RNA/DNA Concatemers and Self-Limited Complexes: Structure and Prospects for Therapeutic Applications DOI Creative Commons

Maria A. Kanarskaya,

С. В. Новикова, Alexander A. Lomzov

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(24), P. 5896 - 5896

Published: Dec. 13, 2024

The development of new convenient tools for the design multicomponent nucleic acid (NA) complexes is one challenges in biomedicine and NA nanotechnology. In this paper, we analyzed formation hybrid RNA/DNA concatemers self-limited by a pair oligonucleotides using UV melting, circular dichroism spectroscopy, gel shift assay. Effects size linker between duplex-forming segments on complexes’ shape number subunits were compared systematized RNA/DNA, DNA/DNA, RNA/RNA assemblies. data complex types summarized here as heat maps offer tool constructs. General rules found allow not only designing with desired structures but also purposefully transforming their geometry. A-form double helix studied was confirmed analysis. Moreover, show first time efficient degradation RNA RNase H imidazole. results open up prospects supramolecular nanotechnology, nanomachinery, biomedical applications.

Language: Английский

Citations

0