Single-layer graphene oxide nanosheets induce proliferation and Osteogenesis of single-cell hBMSCs encapsulated in Alginate Microgels
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Oct. 25, 2024
Microfluidics
cell
encapsulation
offers
a
way
to
mimic
3D
microenvironment
that
supports
growth
and
proliferation,
while
also
protecting
cells
from
environmental
stress.
This
technique
has
found
extensive
applications
in
tissue
engineering
therapies.
Several
studies
have
demonstrated
the
advantages
of
graphene
oxide
(GO)
as
an
osteogenic
inducer;
however,
significance
GO
on
stem
fate
single-cell
state
is
still
unclear.
Here,
microfluidics-based
approach
developed
for
continuous
mesenchymal
(MSCs)
at
level
using
alginate
microgels.
So,
single-layer
(slGO)
nanosheet
used
be
encapsulated
inside
droplets.
The
results
AFM
SEM
show
slGO
can
increase
roughness
reduce
stiffness
hydrogels.
Young's
modulus
alginate-slGO
was
obtained
1414
kPa
985.9
kPa,
respectively.
Live/dead
assay
fluorescence
microscopy
images
illustrate
maintain
viability
proliferation
microencapsulated
hBMSCs.
increases
mineralization
hBMSCs,
so
microgels
containing
hBMSCs
gradually
became
opaque
during
21
days
culture.
RT-qPCR
indicate
expression
OCN,
Runx2,
ALP
significantly
higher
than
OCN
Runx2
4.27
5.87-fold
microgels,
It
concluded
low
doses
nanosheets
potential
utilized
development
bone
regeneration.
finding
new
method
creating
injectable
transplants
are
minimally
invasive.
Language: Английский
Bio-Piezoelectric Ceramic Coatings for Bone Tissue Engineering Applications
Advances in chemical and materials engineering book series,
Journal Year:
2024,
Volume and Issue:
unknown, P. 349 - 392
Published: Nov. 22, 2024
This
chapter
is
focused
on
describing
the
latest
advances
related
to
development
of
bio-piezoelectric
coatings
for
bone
regeneration
applications.
It
starts
with
description
main
concepts
about
bioelectrical
phenomena
in
human
body
and
its
role
bone.
explains
such
as
dielectric
electrical
responses
that
includes
piezoelectricity,
pyroelectricity,
ferroelectricity
how
can
present
these
types
phenomena.
The
also
definition
bio-ceramic
materials
bioactive
coatings,
a
summary
employed
nowadays
applications
tissue
regeneration.
Also,
this
review
engineering
future
perspectives
topic.
Overall,
reviewing
comprehensively
response
natural
tissues
relevance
ceramics
Language: Английский