OTX2 Homeoprotein Functions in Adult Choroid Plexus DOI Open Access
Anabelle Planques, Vanessa Oliveira Moreira,

David Benacom

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(16), P. 8951 - 8951

Published: Aug. 19, 2021

The choroid plexus is an important blood barrier that secretes cerebrospinal fluid, which essential for embryonic brain development and adult homeostasis. OTX2 homeoprotein a transcription factor critical remains highly expressed in plexus. Through RNA sequencing analyses of constitutive conditional knockdown mouse models, we reveal putative functional roles function, including cell signaling adhesion, show regulates the expression factors are secreted into notably transthyretin. We also Otx2 impacts immune stress responses, affects splicing, leading to changes mRNA isoforms proteins implicated oxidative response DNA repair. mass spectrometry analysis protein partners plexus, known non-cell-autonomous target regions, such as visual cortex subventricular zone, identify targets involved chromatin structure, processing. Thus, retains regulating function homeostasis throughout life.

Language: Английский

The vasculature of neurogenic niches: Properties and function DOI Creative Commons
Andromachi Karakatsani, María I. Álvarez-Vergara, Carmen Ruiz de Almodóvar

et al.

Cells and Development, Journal Year: 2023, Volume and Issue: 174, P. 203841 - 203841

Published: April 14, 2023

In the adult rodent brain, neural stem cells (NSCs) reside in subventricular zone (SVZ) of lateral ventricles and subgranular (SGZ) hippocampus. these areas, NSCs their progeny integrate intrinsic signals extrinsic cues provided by microenvironment that control behavior. The vasculature SVZ SGZ, choroid plexus (ChP) SVZ, have emerged as critical compartments neurogenic niches they provide a rich repertoire to regulate NSC quiescence, proliferation, self-renewal differentiation. Physical contact between blood vessels is also feature within supports different processes such migration vesicle transport. this review, we description brain both cell niches, highlighting main properties role each niche. We summarize current understanding how vessel- ChP-derived influence behavior physiological adulthood, well upon aging.

Language: Английский

Citations

13
Assessing the Role of Ependymal and Vascular Cells as Sources of Extracellular Cues Regulating the Mouse Ventricular-Subventricular Zone Neurogenic Niche DOI Creative Commons

S. Quaresima,

Arif Istiaq, Hirofumi Jono

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: April 5, 2022

Neurogenesis persists in selected regions of the adult mouse brain; among them, ventricular-subventricular zone (V-SVZ) lateral ventricles represents a major experimental paradigm due to its conspicuous neurogenic output. Postnatal V-SVZ neurogenesis is maintained by resident population neural stem cells (NSCs). Although NSCs are largely quiescent, they can be activated enter cell cycle, self-renew and generate progeny that gives rise olfactory bulb interneurons. These adult-born neurons integrate into existing circuits modify cognitive functions response external stimuli, but shed also reach injured brain regions, suggesting latent regenerative potential. The endowed with specialized microenvironment, which essential maintain proliferative potential NSCs, preserve NSC pool from exhaustion finely tuning their quiescent active states. Intercellular communication paramount niche properties V-SVZ, several extracellular signals acting milieu have been identified. An important part these comes non-neural types, such as local vascular cells, ependymal glial cells. Understanding crosstalk between other components may aid therapeutic approaches for neuropathological conditions, since neurodevelopmental disorders, age-related decline neurodegenerative diseases associated dysfunctional niches. Here, we review recent advances study complex interactions cellular niche. We focus on cues produced regulate behavior postnatal discuss implication molecular pathological conditions.

Language: Английский

Citations

19
OTX‐2 Expression as a Diagnostic Marker for Choroid Plexus Tumors DOI Open Access
Ayça Erşen Danyeli,

Zeynep Hüseyinoğlu,

M. Memet Özek

et al.

Neuropathology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 23, 2025

ABSTRACT Choroid plexus tumors are neuroepithelium‐derived arising in the ventricles of central nervous system. They commonly seen childhood and correspond to low rates all system tumors. Due their rareness similar histomorphologic features other tumors, diagnosis might be challenging. Here, we used OTX‐2 antibody evaluate diagnostic role expression choroid We performed a retrospective review 34 patients operated for our center between 2011 2023. Additionally, as different tumor types also ventricles, selected five cases each AT/RT, germ cell tumor, ependymoma, metastatic adenocarcinoma from pathology archive. Immunohistochemistry conditions were adjusted specific based on manufacturers' recommendations concentrations antigen retrieval/blocking. OTX‐2, S‐100, transthyretin staining was sections case.

Language: Английский

Citations

0
Choroid Plexus: The Orchestrator of Long-Range Signalling Within the CNS DOI Open Access
Karol Kaiser, Vı́tězslav Bryja

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(13), P. 4760 - 4760

Published: July 4, 2020

Cerebrospinal fluid (CSF) is the liquid that fills brain ventricles. CSF represents not only a mechanical protection but also rich source of signalling factors modulating diverse processes during development and adulthood. The choroid plexus (CP) major as such it has recently emerged an important mediator extracellular within brain. Growing interest in CP revealed its capacity to release broad variety bioactive molecules that, via CSF, regulate across whole central nervous system (CNS). Moreover, been recognized sensor, responding altered composition associated with changes patterns CNS activity. In this review, we summarize recent advances our understanding centre mediates long-range communication CNS. By providing detailed account secretory repertoire, describe how contributes regulation environment—in context both embryonal well adult We highlight role regulator function acts CSF-mediated signalling. Further studies CP–CSF hold potential provide key insights into biology CNS, implications for better treatment neuropathological conditions.

Language: Английский

Citations

25
The phenotypic spectrum associated with OTX2 mutations in humans DOI Creative Commons
Louise Gregory, Péter Gergics, Marilena Nakaguma

et al.

European Journal of Endocrinology, Journal Year: 2021, Volume and Issue: 185(1), P. 121 - 135

Published: May 5, 2021

The transcription factor OTX2 is implicated in ocular, craniofacial, and pituitary development.

Language: Английский

Citations

23
The choroid plexus maintains ventricle volume and adult subventricular zone neuroblast pool, which facilitates post-stroke neurogenesis DOI Creative Commons

Александр Таранов,

Alicia Bedolla, Eri Iwasawa

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 23, 2024

Abstract The brain’s neuroreparative capacity after injuries such as ischemic stroke is contained in the neurogenic niches, primarily subventricular zone (SVZ), which lies close contact with cerebrospinal fluid (CSF) produced by choroid plexus (ChP). Despite wide range of their proposed functions, ChP/CSF remain among most understudied compartments central nervous system (CNS). Here we report a mouse genetic tool (the ROSA26iDTR line) for non-invasive, specific, and temporally controllable ablation CSF-producing ChP epithelial cells to assess roles CSF brain homeostasis injury. Using this model, demonstrate that causes rapid permanent volume loss accompanied disruption ependymal cilia bundles. Surprisingly, did not result overt neurological deficits at one-month post-ablation. However, observed pronounced decrease pool SVZ neuroblasts following ablation, occurs due enhanced migration into olfactory bulb. In MCAo model stroke, neuroblast lesion site was also reduced CSF-depleted mice. Thus, our study establishes an important novel role regulating regenerative adult under normal conditions stroke.

Language: Английский

Citations

2
Phenotypic and gene expression features associated with variation in chronic ethanol consumption in heterogeneous stock collaborative cross mice DOI

Robert Hitzemann,

Tamara J. Phillips, Denesa Lockwood

et al.

Genomics, Journal Year: 2020, Volume and Issue: 112(6), P. 4516 - 4524

Published: Aug. 7, 2020

Language: Английский

Citations

17
Current progress and challenges in the development of brain tissue models: How to grow up the changeable brain in vitro? DOI Creative Commons
А. Б. Салмина, Olga P. Alexandrova, Anton S. Averchuk

et al.

Journal of Tissue Engineering, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 1, 2024

In vitro modeling of brain tissue is a promising but not yet resolved problem in modern neurobiology and neuropharmacology. Complexity the structure diversity cell-to-cell communication (patho)physiological conditions make this task almost unachievable. However, establishment novel models would ultimately lead to better understanding development-associated or experience-driven plasticity, designing efficient approaches restore aberrant functioning. The main goal review summarize available data on methodological that are currently use, identify most prospective trends development neurovascular unit, blood-brain barrier, blood-cerebrospinal fluid neurogenic niche models. manuscript focuses regulation adult neurogenesis, cerebral microcirculation fluids dynamics should be reproduced 4D mimic its alterations pathology. We discuss critical for studying deciphering individual person-specific trajectory aging, testing new drug candidates

Language: Английский

Citations

2
Postnatal neuronal migration in health and disease DOI Creative Commons
Chikako Nakajima, Masato Sawada, Kazunobu Sawamoto

et al.

Current Opinion in Neurobiology, Journal Year: 2020, Volume and Issue: 66, P. 1 - 9

Published: July 24, 2020

Language: Английский

Citations

13
Choroid plexus APP regulates adult brain proliferation and animal behavior DOI Creative Commons
Karen Arnaud, Vanessa Oliveira Moreira,

Jean Vincent

et al.

Life Science Alliance, Journal Year: 2021, Volume and Issue: 4(11), P. e202000703 - e202000703

Published: Sept. 20, 2021

Elevated amyloid precursor protein (APP) expression in the choroid plexus suggests an important role for extracellular APP metabolites such as sAPPα cerebrospinal fluid. Despite widespread App brain expression, we hypothesized that specifically targeting could alter animal physiology. Through various genetic and viral approaches adult mouse, show levels significantly impact proliferation both subventricular zone hippocampus dentate gyrus neurogenic niches. Given of Aβ peptides Alzheimer disease pathogenesis, also tested whether favoring production negatively affect niche functions. After AAV5-mediated long-term human mutated wild-type mice, observe reduced proliferation, behavioral defects reversal learning, deficits hippocampal potentiation. Our findings highlight unique played by regulating function suggest may provide a means to improve alleviate disease-related burdens.

Language: Английский

Citations

12