SYT7 as a Potential Prognostic Marker Promotes the Metastasis of Epithelial Ovarian Cancer Cells by Activating the STAT3 Pathway DOI
Y. Li, Fengping Shao, Ying Huang

et al.

Molecular Carcinogenesis, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

ABSTRACT The study aimed to investigate the impact of synaptotagmin 7 (SYT7) on metastasis epithelial ovarian cancer (EOC) and its potential mechanisms. This was achieved through analysis SYT7 expression levels clinical relevance in EOC using bioinformatics from TCGA. Additionally, examined influence migration invasion cells, as well explored molecular mechanisms vitro cell lines vivo mouse xenograft models. Our research revealed that human tissues exhibit significantly elevated compared normal tissues, is inversely correlated with overall survival. Suppression effectively impeded migratory invasive capabilities CAOV3 whereas overexpression notably accelerated tumor progression A2780 cells. Mechanistic investigations demonstrated upregulates p‐STAT3 MMP2 Importantly, treatment STAT3 inhibitor niclosamide counteracted oncogenic effects EOC. inhibition found reduce a nude model. findings suggest upregulation associated negative prognosis, it enhances by activating signaling pathway. Thus, might be utilized prognostic marker target.

Language: Английский

Paternal fenvalerate exposure causes depressive-like behaviour by altering Grb10 gene DNA methylation in adolescent offspring DOI Creative Commons
Huiru Chen, Tao Wang, Jing Shao

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 292, P. 117994 - 117994

Published: March 1, 2025

Fenvalerate, a typical pyrethroid pesticide, is neurological toxicant. This study aimed to evaluate the effect of paternal exposure fenvalerate on depressive-like behaviours in adolescent offspring. Depression-like behavior was determined by Sucrose Preference Test (SPT), Tail Suspension (TST) and Forced Swimming (FST) The level dopamine reduced midbrain fenvalerate-exposed Tyrosine hydroxylase (Th), rate limiting enzyme for synthesis, significantly offspring exposed fenvalerate. And Th decreased hindbrain fetuses Transcriptome analysis revealed growth factor receptor-bound protein 10 (Grb10) fetal Grb10 mRNA were Interestingly, vitro experiments, si-Grb10. Conversely, increased oe-Grb10. Mechanistically, 5mC content gene at one CpG fragment eighteen sites sperm In summary, causes altering DNA methylation sperm.

Language: Английский

Citations

0
Synaptotagmin-1 is a Ca2+ sensor for somatodendritic dopamine release DOI Creative Commons
Joseph J. Lebowitz, Aditi Banerjee, Claire Qiao

et al.

Cell Reports, Journal Year: 2022, Volume and Issue: 42(1), P. 111915 - 111915

Published: Dec. 30, 2022

Modes of somatodendritic transmission range from rapid synaptic signaling to protracted regulation over distance. Somatodendritic dopamine secretion in the midbrain leads D2 receptor-induced modulation neurons on timescale seconds. Temporally imprecise release mechanisms are often presumed be at play, and previous work indeed suggested roles for slow Ca2+ sensors. We here use mouse genetics whole-cell electrophysiology establish that fast sensor synaptotagmin-1 (Syt-1) is important release. Syt-1 ablation strongly reduces stimulus-evoked receptor-mediated inhibitory postsynaptic currents (D2-IPSCs) midbrain. D2-IPSCs evoked by paired stimuli exhibit less depression, high-frequency trains restore Spontaneous independent Syt-1, supporting its exocytotic differ conclude relies leading synchronous response initial stimulus.

Language: Английский

Citations

12
Synaptotagmins 1 and 7 Play Complementary Roles in Somatodendritic Dopamine Release DOI Creative Commons
Takuya Hikima, Paul Witkovsky,

Latika Khatri

et al.

Journal of Neuroscience, Journal Year: 2022, Volume and Issue: 42(19), P. 3919 - 3930

Published: March 31, 2022

The molecular mechanisms underlying somatodendritic dopamine (DA) release remain unresolved, despite the passing of decades since its discovery. Our previous work showed robust DA in submillimolar extracellular Ca

Language: Английский

Citations

5
SYT7 as a Potential Prognostic Marker Promotes the Metastasis of Epithelial Ovarian Cancer Cells by Activating the STAT3 Pathway DOI
Y. Li, Fengping Shao, Ying Huang

et al.

Molecular Carcinogenesis, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

ABSTRACT The study aimed to investigate the impact of synaptotagmin 7 (SYT7) on metastasis epithelial ovarian cancer (EOC) and its potential mechanisms. This was achieved through analysis SYT7 expression levels clinical relevance in EOC using bioinformatics from TCGA. Additionally, examined influence migration invasion cells, as well explored molecular mechanisms vitro cell lines vivo mouse xenograft models. Our research revealed that human tissues exhibit significantly elevated compared normal tissues, is inversely correlated with overall survival. Suppression effectively impeded migratory invasive capabilities CAOV3 whereas overexpression notably accelerated tumor progression A2780 cells. Mechanistic investigations demonstrated upregulates p‐STAT3 MMP2 Importantly, treatment STAT3 inhibitor niclosamide counteracted oncogenic effects EOC. inhibition found reduce a nude model. findings suggest upregulation associated negative prognosis, it enhances by activating signaling pathway. Thus, might be utilized prognostic marker target.

Language: Английский

Citations

0