Altered brain-wide auditory networks in a zebrafish model of fragile X syndrome

BMC Biology, Journal Year: 2020, Volume and Issue: 18(1)

Published: Sept. 16, 2020

Abstract Background Loss or disrupted expression of the FMR1 gene causes fragile X syndrome (FXS), most common monogenetic form autism in humans. Although disruptions sensory processing are core traits FXS and autism, neural underpinnings these phenotypes poorly understood. Using calcium imaging to record from entire brain at cellular resolution, we investigated neuronal responses visual auditory stimuli larval zebrafish, using fmr1 mutants model FXS. The purpose this study was alterations networks, brain-wide that underlie aspects autism. Results Combining functional analyses with neurons’ anatomical positions, found −/− animals have normal motion. However, there were several animals. Auditory more plentiful hindbrain structures thalamus. thalamus, torus semicircularis, tegmentum had clusters neurons responded strongly Functional connectivity networks showed inter-regional lower sound intensities (a − 3 6 dB shift) larvae compared wild type. Finally, decoding capacities specific components ascending pathway altered: octavolateralis nucleus within significantly stronger amplitude while telencephalon weaker mutants. Conclusions We demonstrated hypersensitive sound, a 3–6 shift sensitivity, identified four sub-cortical regions and/or greater response strengths stimuli. also constructed an experimentally supported how information may be processed larvae. Our suggests early transmits information, less filtering modulation,

Language: Английский

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Brain-wide visual habituation networks in wild type and fmr1 zebrafish

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Feb. 16, 2022

Abstract Habituation is a form of learning during which animals stop responding to repetitive stimuli, and deficits in habituation are characteristic several psychiatric disorders. Due technical challenges, the brain-wide networks mediating poorly understood. Here we report calcium imaging larval zebrafish repeated visual looming stimuli. We show that different functional categories loom-sensitive neurons located locations throughout brain, both properties their resulting behavior can be modulated by stimulus saliency timing. Using graph theory, identify circuit habituates minimally, moderately habituating midbrain population proposed mediate sensorimotor transformation, downstream elements responsible for higher order representations delivery behavior. Zebrafish larvae carrying mutation fmr1 gene have systematic shift toward sustained premotor activity this network, slower behavioral habituation.

Language: Английский

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The Visual Systems of Zebrafish

Annual Review of Neuroscience, Journal Year: 2024, Volume and Issue: 47(1), P. 255 - 276

Published: April 25, 2024

The zebrafish visual system has become a paradigmatic preparation for behavioral and systems neuroscience. Around 40 types of retinal ganglion cells (RGCs) serve as matched filters stimulus features, including light, optic flow, prey, objects on collision course. RGCs distribute their signals via axon collaterals to 12 retinorecipient areas in forebrain midbrain. major visuomotor hub, the tectum, harbors nine RGC input layers that combine information multiple features. retinotopic map tectum is locally adapted scene statistics subfield–specific demands. Tectal projections premotor centers are topographically organized according commands. known connectivity more than 20 processing streams allows us dissect cellular basis elementary perceptual cognitive functions. Visually evoked responses, such prey capture or loom avoidance, controlled by dedicated multistation pathways that—at least larva—resemble labeled lines. This architecture serves neuronal code's purpose driving adaptive behavior.

Language: Английский

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Motor Behavior Selectively Inhibits Hair Cells Activated by Forward Motion in the Lateral Line of Zebrafish

Current Biology, Journal Year: 2019, Volume and Issue: 30(1), P. 150 - 157.e3

Published: Dec. 19, 2019

How do sensory systems disambiguate events in the external world from signals generated by animal's own motor actions? One strategy is to use an "efference copy" of command inhibit input caused active behavior [1von Holst E. Mittelstaedt H. Das reafferenzprinzip: wechselwirkungen zwischen zentralnervensystem und peripherie.Naturwissenschaften. 1950; 37: 464-476Crossref Scopus (1560) Google Scholar]. But does inhibition self-generated inputs also block transmission stimuli? We investigated this question lateral line, a system that allows fish and amphibians detect water currents contributes behaviors such as rheotaxis [2Oteiza P. Odstrcil I. Lauder G. Portugues R. Engert F. A novel mechanism for mechanosensory-based larval zebrafish.Nature. 2017; 547: 445-448Crossref PubMed (75) Scholar] predator avoidance [3McHenry M.J. Feitl K.E. Strother J.A. Van Trump W.J. Larval zebrafish rapidly sense flow predator's strike.Biol. Lett. 2009; 5: 477-479Crossref (106) Scholar, 4Olszewski J. Haehnel M. Taguchi Liao J.C. Zebrafish larvae exhibit can escape continuous suction source using their line.PLoS ONE. 2012; 7: e36661Crossref (63) This mechanical begins hair cells grouped into neuromasts dotted along body [5Pujol-Martí López-Schier Developmental architectural principles lateral-line neural map.Front. Neural Circuits. 2013; 47Crossref (28) Each neuromast contains two populations cells, activated deflection either anterior or posterior direction [6Görner Untersuchungen zur morphologie elektrophysiologie des seitenlinienorgans vom krallenfrosch (Xenopus laevis Daudin).Z. Vgl. Physiol. 1963; 47: 316-338Crossref (99) Scholar], well efferent fibers are during suppress afferents projecting brain [7Russell I.J. Influence fibres on receptor.Nature. 1968; 219: 177-178Crossref (36) 8Russell The role Xenopus laevis.J. Exp. Biol. 1971; 54: 621-641Crossref 9Roberts B.L. Russell activity neurones stationary swimming dogfish.J. 1972; 57: 435-448PubMed 10Russell Roberts Active reduction sensitivity Comp. 1974; 94: 7-15Crossref (42) 11Bricaud O. Chaar V. Dambly-Chaudière C. Ghysen A. Early innervation line.J. Neurol. 2001; 434: 253-261Crossref 12Dow Jacobo Hossain S. Siletti K. Hudspeth A.J. Connectomics zebrafish's reveals wiring miswiring simple microcircuit.eLife. 2018; 1193Crossref (19) To test how far efference copy signal modulates responses stimuli, we imaged synaptic fictive swimming. find efferents transmit precise single spike nerve be associated with ∼50% glutamate release. acted high selectivity polarized deflections, would occur forward motion. During swimming, therefore, "push-pull" encoding stimulus opposite polarity disrupted while still allowing subset stimuli originating world.

Language: Английский

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Calcium Imaging and the Curse of Negativity

Frontiers in Neural Circuits, Journal Year: 2021, Volume and Issue: 14

Published: Jan. 6, 2021

The imaging of neuronal activity using calcium indicators has become a staple modern neuroscience. However, without ground truths, there is real risk missing significant portion the responses. Here, we show that common assumption, non-negativity responses as detected by indicators, biases all levels frequently used analytical methods for these data. From extraction meaningful fluorescence changes to spike inference and analysis inferred spikes, each step risks because assumption non-negativity. We first negative deviations from baseline can exist in activity. Then, use simulated data test three popular algorithms image analysis, CaImAn, suite2p, CellSort, finding suite2p may be best suited large datasets. also tested included Cellsort, well dedicated MLspike CASCADE, found have limitations dealing with inhibited neurons. Among algorithms, FOOPSI, performed on neurons, but even this algorithm spurious spikes upon return signal baseline. As such, new approaches will needed before sensitively accurately further suggest avoiding that, assuming non-negativity, ignore Instead, exploratory step, k-means or PCA example, detect whether are present. Taking steps ensure inhibition, excitation,

Language: Английский

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Microfluidics for understanding model organisms

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: June 9, 2022

Abstract New microfluidic systems for whole organism analysis and experimentation are catalyzing biological breakthroughs across many fields, from human health to fundamental biology principles. This perspective discusses recent tools study intact model organisms demonstrate the tremendous potential these integrated approaches now into future. We describe microsystems' technical features highlight unique advantages precise manipulation in areas including immobilization, automated alignment, sorting, sensory, mechanical chemical stimulation, genetic thermal perturbation. Our aim is familiarize technologically focused researchers with microfluidics applications research, while providing biologists an entrée advanced microengineering techniques organisms.

Language: Английский

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Structure and function in artificial, zebrafish and human neural networks

Physics of Life Reviews, Journal Year: 2023, Volume and Issue: 45, P. 74 - 111

Published: April 25, 2023

Language: Английский

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Diving into the zebrafish brain: exploring neuroscience frontiers with genetic tools, imaging techniques, and behavioral insights

Frontiers in Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 17

Published: March 12, 2024

The zebrafish ( Danio rerio ) is increasingly used in neuroscience research. Zebrafish are relatively easy to maintain, and their high fecundity makes them suitable for high-throughput experiments. Their small, transparent embryos larvae allow microscopic imaging of the developing brain. also share a degree genetic similarity with humans, amenable manipulation techniques, such as gene knockdown, knockout, or knock-in, which allows researchers study role specific genes relevant human brain development, function, disease. can serve model behavioral studies, including locomotion, learning, social interactions. In this review, we present state-of-the-art methods function zebrafish, tools labeling single neurons neuronal circuits, live neural activity, synaptic dynamics protein interactions brain, optogenetic manipulation, use virtual reality technology testing. We highlight potential research, especially regarding genetic-based disorders discuss its certain limitations model.

Language: Английский

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How Zebrafish Can Drive the Future of Genetic-based Hearing and Balance Research

Journal of the Association for Research in Otolaryngology, Journal Year: 2021, Volume and Issue: 22(3), P. 215 - 235

Published: April 28, 2021

Abstract Over the last several decades, studies in humans and animal models have successfully identified numerous molecules required for hearing balance. Many of these relied on unbiased forward genetic screens based behavior or morphology to identify molecules. Alongside screens, reverse genetics has further driven exploration candidate This review provides an overview that established zebrafish as a model balance research. Further, we discuss how unique advantages can be leveraged future studies. We explore strategies design novel morphological alterations using transgenic lines behavioral changes following mechanical acoustic damage. also outline recent advances CRISPR-Cas9 applied perform validate large sequencing datasets. Overall, this describes continue advance our understanding inherited acquired disorders .

Language: Английский

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Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy

Biomedical Optics Express, Journal Year: 2021, Volume and Issue: 13(3), P. 1516 - 1516

Published: Nov. 26, 2021

Light-sheet fluorescence microscopy (LSFM) enables real-time whole-brain functional imaging in zebrafish larvae. Conventional one-photon LSFM can however induce undesirable visual stimulation due to the use of visible excitation light. The two-photon (2P) excitation, employing near-infrared invisible light, provides unbiased investigation neuronal circuit dynamics. However, low efficiency 2P absorption process, speed this technique is typically limited by signal-to-noise-ratio. Here, we describe a setup designed for non-invasive that quintuplicating state-of-the-art volumetric acquisition rate larval brain (5 Hz) while keeping laser intensity on specimen. We applied our system study pharmacologically-induced acute seizures, characterizing spatial-temporal dynamics pathological activity and describing first time appearance caudo-rostral ictal waves (CRIWs).

Language: Английский

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