Coordinated spinal locomotor network dynamics emerge from cell-type-specific connectivity patterns DOI Creative Commons
F. David Wandler,

Benjamin K Lemberger,

David L. McLean

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 21, 2024

Even without detailed instruction from the brain, spinal locomotor circuitry generates coordinated behavior characterized by left-right alternation, segment-to-segment propagation, and variable-speed control. While existing models have emphasized contributions of cellular- network-level properties, core mechanisms underlying rhythmogenesis remain incompletely understood. Further, neither family has fully accounted for recent experimental results in zebrafish other organisms pointing to importance cell-type-specific intersegmental connectivity patterns recruitment speed-selective subpopulations interneurons. Informed these findings others, we developed a hierarchy increasingly network. We find that locomotion emerges an inhibition-dominated network which is determined phase relationships among interneurons control implemented subpopulations. while structured excitatory connections are not necessary rhythmogenesis, they useful increasing peak frequency, albeit at cost smooth transitions intermediate frequencies, suggesting basic computational trade-off between speed Together, this shows interactions sufficient generate coordinated, locomotion, providing new interpretations inhibitory connectivity, as well basic, recruitment-based mechanism

Language: Английский

Cell-type-specific origins of locomotor rhythmicity at different speeds in larval zebrafish DOI Creative Commons
Moneeza A. Agha, S. Kishore, David L. McLean

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Feb. 7, 2024

Different speeds of locomotion require heterogeneous spinal populations, but a common mode rhythm generation is presumed to exist. Here, we explore the cellular versus synaptic origins rhythmicity at different by performing electrophysiological recordings from premotor excitatory interneurons in larval zebrafish. Chx10-labeled V2a neurons are divided into least two morphological subtypes proposed play distinct roles timing and intensity control. Consistent with generating output patterning functions within population, find that descending recruited exclusively slow or fast exhibit intrinsic properties suitable for rhythmogenesis those speeds, while bifurcating more reliably all lack appropriate rhythmogenic properties. Unexpectedly, however, phasic firing patterns during non-rhythmogenic alike best explained modes inhibition linked cell type speed. At reciprocal supports firing, recurrent helps pattern motor output. In contrast, rely on alone Our findings suggest cell-type-specific, not common, generate coordinate locomotion.

Language: Английский

Citations

2
Cell-type-specific origins of spinal rhythmicity at different locomotor speeds in larval zebrafish DOI Open Access
Moneeza A. Agha, S. Kishore, David L. McLean

et al.

Published: Feb. 7, 2024

Different speeds of locomotion require heterogeneous spinal populations, but a common mode rhythm generation is presumed to exist. Here, we explore the cellular versus synaptic origins rhythmicity at different by performing electrophysiological recordings from premotor excitatory interneurons in larval zebrafish. Chx10-labeled V2a neurons are divided into least two subtypes proposed play distinct roles timing and intensity control. Consistent with generating output patterning functions within population, find that one subtype recruited exclusively slow or fast exhibits intrinsic properties suitable for rhythmogenesis those speeds, while other more reliably all lacks appropriate rhythmogenic properties. Unexpectedly, however, phasic firing patterns during non-rhythmogenic best explained modes inhibition linked cell-type speed. At reciprocal supports firing, recurrent helps pattern motor output. In contrast, rely on alone Our findings suggest cell-type-specific, not common, generate coordinate locomotion.

Language: Английский

Citations

0
Cell-type-specific origins of locomotor rhythmicity at different speeds in larval zebrafish DOI Creative Commons
Moneeza A. Agha, S. Kishore, David L. McLean

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Sept. 17, 2024

Different speeds of locomotion require heterogeneous spinal populations, but a common mode rhythm generation is presumed to exist. Here, we explore the cellular versus synaptic origins rhythmicity at different by performing electrophysiological recordings from premotor excitatory interneurons in larval zebrafish. Chx10-labeled V2a neurons are divided into least two morphological subtypes proposed play distinct roles timing and intensity control. Consistent with generating output patterning functions within population, find that descending recruited exclusively slow or fast exhibit intrinsic properties suitable for rhythmogenesis those speeds, while bifurcating more reliably all lack appropriate rhythmogenic properties. Unexpectedly, however, phasic firing patterns during non-rhythmogenic alike best explained modes inhibition linked cell type speed. At reciprocal supports firing, recurrent helps pattern motor output. In contrast, rely on alone Our findings suggest cell-type-specific, not common, generate coordinate locomotion.

Language: Английский

Citations

0
Cell-type-specific origins of locomotor rhythmicity at different speeds in larval zebrafish DOI Creative Commons
Moneeza A. Agha, S. Kishore, David L. McLean

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 12, 2024

Different speeds of locomotion require heterogeneous spinal populations, but a common mode rhythm generation is presumed to exist. Here, we explore the cellular versus synaptic origins rhythmicity at different by performing electrophysiological recordings from premotor excitatory interneurons in larval zebrafish. Chx10-labeled V2a neurons are divided into least two morphological subtypes proposed play distinct roles timing and intensity control. Consistent with generating output patterning functions within population, find that descending recruited exclusively slow or fast exhibit intrinsic properties suitable for rhythmogenesis those speeds, while bifurcating more reliably all lack appropriate rhythmogenic properties. Unexpectedly, however, phasic firing patterns during non-rhythmogenic alike best explained modes inhibition linked cell-type speed. At reciprocal supports firing, recurrent helps pattern motor output. In contrast, rely on alone Our findings suggest cell-type-specific, not common, generate coordinate locomotion.

Language: Английский

Citations

0
Cell-type-specific origins of locomotor rhythmicity at different speeds in larval zebrafish DOI Open Access
Moneeza A. Agha, S. Kishore, David L. McLean

et al.

Published: July 11, 2024

Different speeds of locomotion require heterogeneous spinal populations, but a common mode rhythm generation is presumed to exist. Here, we explore the cellular versus synaptic origins rhythmicity at different by performing electrophysiological recordings from premotor excitatory interneurons in larval zebrafish. Chx10-labeled V2a neurons are divided into least two morphological subtypes proposed play distinct roles timing and intensity control. Consistent with generating output patterning functions within population, find that descending recruited exclusively slow or fast exhibit intrinsic properties suitable for rhythmogenesis those speeds, while bifurcating more reliably all lack appropriate rhythmogenic properties. Unexpectedly, however, phasic firing patterns during non-rhythmogenic alike best explained modes inhibition linked cell-type speed. At reciprocal supports firing, recurrent helps pattern motor output. In contrast, rely on alone Our findings suggest cell-type-specific, not common, generate coordinate locomotion.

Language: Английский

Citations

0
Coordinated spinal locomotor network dynamics emerge from cell-type-specific connectivity patterns DOI Creative Commons
F. David Wandler,

Benjamin K Lemberger,

David L. McLean

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 21, 2024

Even without detailed instruction from the brain, spinal locomotor circuitry generates coordinated behavior characterized by left-right alternation, segment-to-segment propagation, and variable-speed control. While existing models have emphasized contributions of cellular- network-level properties, core mechanisms underlying rhythmogenesis remain incompletely understood. Further, neither family has fully accounted for recent experimental results in zebrafish other organisms pointing to importance cell-type-specific intersegmental connectivity patterns recruitment speed-selective subpopulations interneurons. Informed these findings others, we developed a hierarchy increasingly network. We find that locomotion emerges an inhibition-dominated network which is determined phase relationships among interneurons control implemented subpopulations. while structured excitatory connections are not necessary rhythmogenesis, they useful increasing peak frequency, albeit at cost smooth transitions intermediate frequencies, suggesting basic computational trade-off between speed Together, this shows interactions sufficient generate coordinated, locomotion, providing new interpretations inhibitory connectivity, as well basic, recruitment-based mechanism

Language: Английский

Citations

0