Electroacupuncture Regulates Cannabinoid Receptor 1 Expression in a Mouse Fibromyalgia Model: Pharmacological and Chemogenetic Modulation DOI Creative Commons
Yu-An Yeh, Hsin‐Cheng Hsu,

Ming‐Chia Lin

et al.

Life, Journal Year: 2024, Volume and Issue: 14(11), P. 1499 - 1499

Published: Nov. 17, 2024

Fibromyalgia is a chronic illness usually accompanied by long-lasting, general pain throughout the body, often anxiety, depression, fatigue, and sleep disruption. Meanwhile, doctors scientists have not entirely discovered detailed mechanisms; patients always an exaggerated sensation to pervasive without satisfied medical service. Given lack of knowledge on its underlying mechanism, current treatments aim provide and/or symptom relief. The present study aimed clarify role cannabinoid receptor 1 (CB1) signaling in mouse fibromyalgia model. To develop model, mice were subjected intermittent cold stress (ICS). Our results indicated that mechanical (2.09 ± 0.09 g) thermal hyperalgesia (4.77 0.29 s), which evaluated von Frey Hargraves’ tests, induced ICS, suggesting successful modeling. hurting replies then provoked electroacupuncture (EA) but for sham EA mice. Further, Western blot analysis, we found significantly decreased CB1 protein levels thalamus, somatosensory cortex, anterior cingulate cortex. In addition, pain-related kinases transcription factor increased. Treatment with reliably increased expression various brain regions sequentially alleviated nociceptive mediators. Furthermore, administration agonist attenuated pain, reversed analgesia antagonist, further chemogenetic inhibition SSC. innovative findings evidence interaction fibromyalgia, potential clinical trials as treatment target.

Language: Английский

Nitric oxide-signalling affects panic-like defensive behaviour and defensive antinociception neuromodulation in the prelimbic cerebral cortex DOI
Renato Leonardo de Freitas,

Renata Moreira Acunha,

Fernando René Bendaña-Córdoba

et al.

Brain Research, Journal Year: 2024, Volume and Issue: 1844, P. 149134 - 149134

Published: Aug. 7, 2024

Language: Английский

Citations

1
The analgesic effects of Yu-Xue-Bi tablet (YXB) on mice with inflammatory pain by regulating LXA4-FPR2-TRPA1 pathway DOI Creative Commons
Ying Liu, Guoxin Zhang,

Chunyan Zhu

et al.

Chinese Medicine, Journal Year: 2024, Volume and Issue: 19(1)

Published: Aug. 6, 2024

Abstract Background Oxylipins including lipoxin A4 (LXA4) facilitate the resolution of inflammation and possess analgesic properties by inhibiting macrophage infiltration transient receptor potential (TRP) protein expression. Yu-Xue-Bi Tablet (YXB) is a traditional Chinese patent medicine used to relieve inflammatory pain. Our previous research has shown that effect YXB related peripheral regulating infiltration, but mechanism not yet clear. The purpose this study explore mechanisms on mice models with Complete Freund’s Adjuvant (CFA)-induced pain from perspective at inflammation. Methods Mechanical allodynia thresholds heat hypersensitivity were measured using Von Frey test hot plate respectively. open field tail suspension employed measure anxiety depressive behaviors expression CD68 + proportion F4/80 CD11b cells immunofluorescence staining flow cytometry. ankyrin 1(TRPA1) was western blotting. omics analysis provided quantitative data oxylipins in paws, enzyme linked immunosorbent assay (ELISA) levels LXA4 there. Immunofluorescence perform Leukotriene hydroxylase (LTA4H) paws mice. impact injecting formyl peptide 2(FPR2) antagonist WRW4 TRPA1 agonist AITC into left observed, focusing mechanical thresholds, , Calcitonin gene-related (CGRP) L5 spinal dorsal horn. Results elevated alleviated CFA It significantly reduced number within thereby decreasing infiltration. Additionally, it diminished TRPV1 DRG, leading an inhibition sensitization. Through analysis, found could modulate DHA-derived LXA4. ELISA results indicated inhibited LAT4H paws. Furthermore, pro-resolution effects hindered after administration FPR2 antagonist. Compared group, showed no significant improvement anti-inflammatory effects. Conclusions can regulate promote LXA4-FPR2-TRPA1 pathway key for

Language: Английский

Citations

0
Pituitary adenylate cyclase-activating polypeptide (PACAP) overexpression in the paraventricular nucleus of the thalamus alters motivated and affective behavior in female rats DOI

Brody A. Carpenter,

Breanne E. Pirino,

Malcolm C. Jennings

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 2, 2024

ABSTRACT Background Pituitary adenylate cyclase-activating polypeptide (PACAP) has been found to be involved in a wide range of motivated and affective behaviors. While the PACAP-38 isoform is more densely expressed than PACAP-27 most brain, highly rodent paraventricular nucleus thalamus (PVT), where females also have greater expression males. Notably, role cells PVT not explored. Methods Adult, female Long-Evans rats were injected with an AAV increase PACAP or control AAV. They then investigated for subsequent gene peptide levels PVT; ethanol drinking preference; sucrose locomotor activity novel chamber, behavior light-dark box, novelty suppression feeding test, familiar forced swim test. Results Gene was significantly increased by four weeks after injection AAV, this resulted specific PACAP-27. Rats demonstrated reduced preference under intermittent-access procedure compared those In contrast, showed no significant difference sucrose, any tested, except that they spent less time swimming Conclusions light low overall level ability drinking, minimal effects on other behaviors, supports idea compounds related should as potential therapeutics treatment alcohol use disorder.

Language: Английский

Citations

0
Directions for analgesic development DOI Creative Commons

Ting Ge,

Wu Zhou,

Sha Sha

et al.

Journal of Anesthesia and Translational Medicine, Journal Year: 2024, Volume and Issue: 3(3), P. 87 - 95

Published: Sept. 1, 2024

Language: Английский

Citations

0
Electroacupuncture Regulates Cannabinoid Receptor 1 Expression in a Mouse Fibromyalgia Model: Pharmacological and Chemogenetic Modulation DOI Creative Commons
Yu-An Yeh, Hsin‐Cheng Hsu,

Ming‐Chia Lin

et al.

Life, Journal Year: 2024, Volume and Issue: 14(11), P. 1499 - 1499

Published: Nov. 17, 2024

Fibromyalgia is a chronic illness usually accompanied by long-lasting, general pain throughout the body, often anxiety, depression, fatigue, and sleep disruption. Meanwhile, doctors scientists have not entirely discovered detailed mechanisms; patients always an exaggerated sensation to pervasive without satisfied medical service. Given lack of knowledge on its underlying mechanism, current treatments aim provide and/or symptom relief. The present study aimed clarify role cannabinoid receptor 1 (CB1) signaling in mouse fibromyalgia model. To develop model, mice were subjected intermittent cold stress (ICS). Our results indicated that mechanical (2.09 ± 0.09 g) thermal hyperalgesia (4.77 0.29 s), which evaluated von Frey Hargraves’ tests, induced ICS, suggesting successful modeling. hurting replies then provoked electroacupuncture (EA) but for sham EA mice. Further, Western blot analysis, we found significantly decreased CB1 protein levels thalamus, somatosensory cortex, anterior cingulate cortex. In addition, pain-related kinases transcription factor increased. Treatment with reliably increased expression various brain regions sequentially alleviated nociceptive mediators. Furthermore, administration agonist attenuated pain, reversed analgesia antagonist, further chemogenetic inhibition SSC. innovative findings evidence interaction fibromyalgia, potential clinical trials as treatment target.

Language: Английский

Citations

0