Identification of the Novel Tumor Suppressor Role of FOCAD/miR-491-5p to Inhibit Cancer Stemness, Drug Resistance and Metastasis via Regulating RABIF/MMP Signaling in Triple Negative Breast Cancer DOI Creative Commons
Wei-Chieh Huang, Hsiang‐Cheng Chi, Shiao-Lin Tung

et al.

Cells, Journal Year: 2021, Volume and Issue: 10(10), P. 2524 - 2524

Published: Sept. 24, 2021

Triple negative breast cancer (TNBC) possesses poor prognosis mainly due to development of chemoresistance and lack effective endocrine or targeted therapies. MiR-491-5p has been found play a tumor suppressor role in many cancers including cancer. However, the precise miR-491-5p TNBC never elucidated. In this study, we reported novel function FOCAD/miR-491-5p TNBC. High expression was be associated with better overall survival patients. We that could an intronic microRNA processed form

Language: Английский

Control of Selective mRNA Translation in Neuronal Subcellular Compartments in Health and Disease DOI Creative Commons

Roberta Cagnetta,

John G. Flanagan, Nahum Sonenberg

et al.

Journal of Neuroscience, Journal Year: 2023, Volume and Issue: 43(44), P. 7247 - 7263

Published: Nov. 1, 2023

In multiple cell types, mRNAs are transported to subcellular compartments, where local translation enables rapid, spatially localized, and specific responses external stimuli. Mounting evidence has uncovered important roles played by in vivo axon survival, regeneration, neural wiring, as well strong links between dysregulation of neurologic disorders. Omic studies have revealed that >1000 present can be selectively locally translated the presynaptic postsynaptic compartments from development adulthood . A large proportion is specifically upregulated or downregulated response distinct extracellular signals. Given translatome large, translated, cue-specifically remodeled, a fundamental question concerns how selective achieved locally. Here, we review emerging regulatory mechanisms neuronal their mRNA targets, orchestration. We discuss remain unexplored. Finally, describe clinical implications potential therapeutic strategies light latest advances gene therapy.

Language: Английский

Citations

10
Essential function and targets of BMP signaling during midbrain neural crest delamination DOI
Michael L. Piacentino, Erica J. Hutchins, Marianne Bronner‐Fraser

et al.

Developmental Biology, Journal Year: 2021, Volume and Issue: 477, P. 251 - 261

Published: June 6, 2021

Language: Английский

Citations

17
RNA localization in confined cells depends on cellular mechanical activity and contributes to confined migration DOI Creative Commons

Rebecca A. Moriarty,

Stavroula Mili, Kimberly M. Stroka

et al.

iScience, Journal Year: 2022, Volume and Issue: 25(2), P. 103845 - 103845

Published: Feb. 1, 2022

Cancer cells experience mechanical confining forces during metastasis and, consequently, can alter their migratory mechanisms. Localization of numerous mRNAs to cell protrusions contributes polarization and migration is controlled by proteins that bind RNA and/or cytoskeletal elements, such as the adenomatous polyposis coli (APC). Here, we demonstrate peripheral localization APC-dependent RNAs in within confined microchannels type dependent. This varying phenotype determined levels a detyrosinated tubulin network. We show this network regulated mechanoactivity with mechanosensitive ion channels increased myosin II activity direct RAB13 RNA. Through specific mislocalization RNA, migration. Our results indicate cell's determines its ability peripherally target utilize them for movement confinement.

Language: Английский

Citations

10
APC couples neuronal mRNAs to multiple kinesins, EB1, and shrinking microtubule ends for bidirectional mRNA motility DOI Creative Commons
Sebastian Baumann, Julia Grawenhoff, Elsa Charas Rodrigues

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(50)

Published: Dec. 5, 2022

Understanding where in the cytoplasm mRNAs are translated is increasingly recognized as being important knowing timing and level of protein expression. localized via active motor-driven transport along microtubules (MTs) but underlying essential factors dynamic interactions largely unknown. Using biochemical vitro reconstitutions with purified mammalian proteins, multicolor TIRF-microscopy, interaction kinetics measurements, we show that adenomatous polyposis coli (APC) enables kinesin-1- kinesin-2-based mRNA transport, APC an ideal adaptor for long-range it forms highly stable complexes 3′UTR fragments several neuronal (APC–RNPs). The kinesin-1 KIF5A binds transports mRNP components such FMRP, PURα weakly, whereas frequency significantly increased by APC. APC–RNP-motor can assemble on MTs, generating processive events. We further find end-binding 1 (EB1) recruits APC–RNPs to dynamically growing MT ends track shrinking producing minus-end-directed RNA motility due high dwell times MTs. Our findings establish a versatile mRNA-kinesin key factor assembly bidirectional movement mRNPs.

Language: Английский

Citations

10
Identification of the Novel Tumor Suppressor Role of FOCAD/miR-491-5p to Inhibit Cancer Stemness, Drug Resistance and Metastasis via Regulating RABIF/MMP Signaling in Triple Negative Breast Cancer DOI Creative Commons
Wei-Chieh Huang, Hsiang‐Cheng Chi, Shiao-Lin Tung

et al.

Cells, Journal Year: 2021, Volume and Issue: 10(10), P. 2524 - 2524

Published: Sept. 24, 2021

Triple negative breast cancer (TNBC) possesses poor prognosis mainly due to development of chemoresistance and lack effective endocrine or targeted therapies. MiR-491-5p has been found play a tumor suppressor role in many cancers including cancer. However, the precise miR-491-5p TNBC never elucidated. In this study, we reported novel function FOCAD/miR-491-5p TNBC. High expression was be associated with better overall survival patients. We that could an intronic microRNA processed form

Language: Английский

Citations

13