A high sugar diet, but not obesity, reduces female fertility inDrosophila melanogaster DOI Open Access
Rodrigo Dutra Nunes, Daniela Drummond‐Barbosa

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: March 3, 2023

ABSTRACT Many studies from Drosophila to humans show a strong link between obesity and reduced fertility. However, is often induced by changes in diet or eating behavior, such that it remains unclear whether fertility consequence of itself, diet, both. Here, we report high sugar reduces female increasing death early germline cysts (prior follicle formation) degeneration vitellogenic follicles; itself does not impair fertility; glucose levels closely correlate with on diet. Females rapidly develop (and display glycogen, glucose, trehalose levels, insulin resistance) decreased In stark contrast high-sugar-obese females, females which similar are adipocyte-specific knockdown anti-obesity genes brummer adipose have normal metabolic indicators. Remarkably, supplemented separate source water also despite persistent obesity, glycogen resistance markers. These results strengthen our conclusion fertility, an inverse correlation demonstrate signaling remain sufficiently maintain insulin-dependent processes during oogenesis irrespective

Language: Английский

Aging and memory are altered by genetically manipulating lactate dehydrogenase in the neurons or glia of flies DOI Creative Commons
Ariel K. Frame, J. Wesley Robinson, Nader H. Mahmoudzadeh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Oct. 21, 2022

Abstract The astrocyte-neuron lactate shuttle hypothesis posits that glial-generated is transported to neurons fuel metabolic processes required for long-term memory. Although studies in vertebrates have revealed shuttling important cognitive function, it uncertain if this form of coupling conserved invertebrates or influenced by age. Lactate dehydrogenase (Ldh) a rate limiting enzyme interconverts and pyruvate. Here we genetically manipulated expression Drosophila melanogaster (dLdh) glia assess the impact altered metabolism on invertebrate aging courtship memory at different ages. We also assessed survival, negative geotaxis, brain neutral lipids (the core component lipid droplets) metabolites. Both upregulation downregulation dLdh resulted decreased survival impairment with Glial caused age-related without altering while upregulated glial lowered disrupting neuronal increased accumulation. provide evidence age affects tricarboxylic acid (TCA) cycle, 2-hydroxyglutarate (2HG), Collectively, our findings indicate direct alteration either but only an age-dependent manner. Author Summary composed metabolically demanding cell types must remain functional throughout life maintain tasks like formation. How varies between cell-types across lifespan uncertain. It has been suggested vertebrate brains breakdown sugars produce support survival. Yet, phenomenon not directly tested invertebrates. In flies, dehydrogenase, central metabolism, specifically within adult brain. discovered shifting higher lower levels worse contrast, found worsened whereas impaired aged flies. led accumulation metabolites, such as 2-hydroxyglutarate, mitochondrial lipids, fly brains. These first time are connected differentially memory, Graphical

Language: Английский

Citations

7
The morphogen Hedgehog is essential for proper adult morphogenesis in Bombyx mori DOI
Kai Chen, Ye Yu, Zhongjie Zhang

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2022, Volume and Issue: 153, P. 103906 - 103906

Published: Dec. 30, 2022

Language: Английский

Citations

7
Cut homeodomain transcription factor is a novel regulator of growth and morphogenesis of cortex glia niche around neural cells DOI
Vaishali Yadav,

Ramkrishna Mishra,

Papri Das

et al.

Genetics, Journal Year: 2023, Volume and Issue: 226(1)

Published: Sept. 26, 2023

Abstract Cortex glia in Drosophila central nervous system form a niche around neural cells for necessary signals to establish cross talk with their surroundings. These grow and expand thin processes cell bodies. Although essential the development function of system, how these make extensive intricate connected networks remains largely unknown. In this study, we show that Cut, homeodomain transcription factor, directly regulates fate cortex glia, impacting stem (NSC) homeostasis. Focusing on thoracic ventral nerve cord, found Cut is required normal growth timely increase DNA content through endocycle later divide via acytokinetic mitosis. Knockdown significantly reduces cellular processes, network NSCs, progeny's Conversely, overexpression induces overall main at expense side ones. Whereas knockdown slows down DNA, results significant nuclear size volume 3-fold glia. Further, note constitutively high also interfered nuclei separation during Since syncytial cells, finding identifies as novel regulator glial variant cycles support functional system.

Language: Английский

Citations

3
Homemade: building the structure of the neurogenic niche DOI Creative Commons

Ghanim Fajish Valamparamban,

Pauline Spéder

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: Dec. 1, 2023

Neural stem/progenitor cells live in an intricate cellular environment, the neurogenic niche, which supports their function and enables neurogenesis. The niche is made of a diversity cell types, including neurons, glia vasculature, are able to signal structurally organised around neural cells. While focus has been on how individual types influence behaviour cells, very little actually known assembled during development from multiple origins, role resulting topology these This review proposes draw state-of-the art picture this emerging field research, with aim expose our knowledge architecture formation different animal models (mouse, zebrafish fruit fly). We will span its aspects, existence importance local, adhesive interactions potential emergence larger-scale topological properties through careful assembly diverse acellular components.

Language: Английский

Citations

3
Metabolic enhancement of mammalian developmental pausing DOI Open Access
Vera A. van der Weijden,

Maximilian Stoetzel,

Beatrix Fauler

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Aug. 23, 2022

Abstract The quest to model and modulate embryonic development became a recent cornerstone of stem cell developmental biology. Mammalian timing is adjustable in vivo by preserving preimplantation embryos dormant state called diapause. Inhibition the growth regulator mTOR (mTORi) pauses mouse vitro, yet constraints pause duration are unrecognized. By comparing response extraembryonic cells mTORi-induced pausing, we identified lipid usage as bottleneck pausing. Enhancing fatty acid oxidation (FAO) boosts embryo longevity, while blocking it reduces pausing capacity. Genomic metabolic analyses single point toward deeper FAO-enhanced reveal link between metabolism morphology. Our results lift constraint on vitro survival suggest that may be critical transition relevant for longevity function across tissues. One-Sentence Summary Facilitating carnitine supplementation enhances inhibition-mediated

Language: Английский

Citations

5
Non-autonomous regulation of neurogenesis by extrinsic cues: a Drosophila perspective DOI Creative Commons
Phuong‐Khanh Nguyen, Louise Cheng

Oxford Open Neuroscience, Journal Year: 2022, Volume and Issue: 1

Published: Jan. 1, 2022

Abstract The formation of a functional circuitry in the central nervous system (CNS) requires correct number and subtypes neural cells. In developing brain, stem cells (NSCs) self-renew while giving rise to progenitors that turn generate differentiated progeny. As such, size diversity make up CNS depend on proliferative properties NSCs. fruit fly Drosophila, where process neurogenesis has been extensively investigated, extrinsic factors such as microenvironment NSCs, nutrients, oxygen levels systemic signals have identified regulators NSC proliferation. Here, we review decades work explores how non-autonomously regulate key characteristics quiescence, proliferation termination fly.

Language: Английский

Citations

4
Insights into unique features of Drosophila CYP4G enzymes DOI
Mary Kefi,

Parasyris Konstantinos,

Vasileia Balabanidou

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2023, Volume and Issue: 164, P. 104041 - 104041

Published: Nov. 25, 2023

Language: Английский

Citations

2
Mitochondrial respiration and dynamics of in vivo neural stem cells DOI Creative Commons
Stavroula Petridi, Dnyanesh Dubal, Richa Rikhy

et al.

Development, Journal Year: 2022, Volume and Issue: 149(23)

Published: Nov. 29, 2022

ABSTRACT Neural stem cells (NSCs) in the developing and adult brain undergo many different transitions, tightly regulated by extrinsic intrinsic factors. While role of signalling pathways transcription factors is well established, recent evidence has also highlighted mitochondria as central players NSC behaviour fate decisions. Many aspects cellular metabolism mitochondrial biology change during interact with affect activity chromatin-modifying enzymes. In this Spotlight, we explore vivo findings, primarily from Drosophila mammalian model systems, about that respiration morphology play development function.

Language: Английский

Citations

3
Golgi-to-ER retrograde transport prevents premature differentiation of Drosophila type II neuroblasts via Notch-signal-sending daughter cells DOI Creative Commons
Huanhuan Zhang, Menglong Rui, Zhixin Ma

et al.

iScience, Journal Year: 2023, Volume and Issue: 27(1), P. 108545 - 108545

Published: Nov. 22, 2023

Stem cells are heterogeneous to generate diverse differentiated cell types required for organogenesis; however, the underlying mechanisms that differently maintain these stem not well understood. In this study, we identify Golgi-to-endoplasmic reticulum (ER) retrograde transport specifically maintains type II neuroblasts (NBs) through Notch signaling. We reveal intermediate neural progenitors (INPs), immediate daughter of NBs, provide Delta and function as NB niche. The used by INPs is mainly produced NBs asymmetrically distributed INPs. Blocking leads a decrease in INP number, which reduces activity results premature differentiation NBs. Furthermore, reduction could suppress tumor formation caused Our highlight crosstalk between Golgi-to-ER transport, signaling, niche, fusion an essential step maintaining self-renewal lineage.

Language: Английский

Citations

1
Dietary stress induced macrophage metabolic reprogramming, a determinant of animal growth DOI Creative Commons
Anusree Mahanta, Sajad Ahmad Najar, Nivedita Hariharan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 21, 2024

Abstract Nutrient sensing and signaling play pivotal roles in animal growth. However, under dietary stress, this system falters, leading to growth defects. While immune cells are increasingly recognized as key nutrient sensors, their impact on remains poorly understood. In study, we investigate how Drosophila larval macrophages respond excessive sugar identify a reconfiguration of metabolic state. They undergo glycolytic shift, intensify TCA activity, elevate TAG synthesis. typical sugarinduced these changes interestingly exert contrasting effects growth: glycolysis increased activity inhibit growth, while the lipogenic shift promotes it. response is insufficient counteract events suppressing resulting an overall reduction adult fly size high conditions. Stimulating pro-lipogenic state facilitates recovery, suggesting paradigm governed by immune-metabolic transitions. This study unveils unexpected influence macrophage reprogramming organismal homeostasis during development, highlighting cell states central determinants particularly stress.

Language: Английский

Citations

0