Mechanobiology and survival strategies of circulating tumor cells: a process towards the invasive and metastatic phenotype

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: May 5, 2023

Metastatic progression is the deadliest feature of cancer. Cancer cell growth, invasion, intravasation, circulation, arrest/adhesion and extravasation require specific mechanical properties to allow survival completion metastatic cascade. Circulating tumor cells (CTCs) come into contact with capillary bed during extravasation/intravasation at beginning However, CTC mechanobiology strategies in bloodstream, specifically microcirculation, are not well known. A fraction CTCs can extravasate colonize distant areas despite biomechanical constriction forces that exerted by microcirculation strongly decrease survival. Furthermore, accumulating evidence shows several adaptations, via molecular factors interactions blood components (e.g., immune platelets inside capillaries), may promote metastasis formation. To better understand journey as part cascade, we reviewed how interaction other types bloodstream help them survive harsh conditions circulatory system metastasize organs.

Language: Английский

---

Circulating Tumor Cells: From Basic to Translational Research

Clinical Chemistry, Journal Year: 2024, Volume and Issue: 70(1), P. 81 - 89

Published: Jan. 1, 2024

Abstract Background Metastasis is the leading cause of cancer-related deaths. Most studies have focused on primary tumor or overt metastatic lesions, leaving a significant knowledge gap concerning blood-borne cancer cell dissemination, major step in cascade. Circulating cells (CTCs) blood patients with solid can now be enumerated and investigated at molecular level, giving unexpected information biology Content Here, we reviewed recent advances basic translational/clinical research CTCs as key elements Summary Findings from translational elucidated complexity process. Fully understanding this process will open new potential avenues for therapeutic diagnostic strategies to propose precision medicine all patients.

Language: Английский

---

Discovery of a sushi domain-containing protein 2-positive phenotype in circulating tumor cells of metastatic breast cancer patients

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 31, 2025

Abstract Cell lines derived from circulating tumor cells (CTCs) in the blood provide important biological information on cancer metastasis. CTC-ITB-01 is a CTC cell line patient with metastatic estrogen receptor-alpha (ER-alpha) positive breast two months before death of patient. After LC-MC/MS based proteomics analysis CTC-ITB-01, we found extraordinary high levels poorly characterized protein SUSD2 (sushi domain-containing 2) CTC-ITB-01. Expression subsets CTCs was validated clinical samples patients cancer. SUSD2-positive could be captured specifically by MACS-based approach. We overexpressed poorly-metastatic MCF-7. This resulted upregulation ER-alpha, progression GRP78 (78-kDa glucose-regulated protein) and downregulation suppressor PDCD4 (programmed 4). observed after hypoxia simulation re-oxygenation MCF-7 MDA-MB-468, while remained unchanged, only downregulated under hypoxia. In conclusion, show, for first time, that expressed appears to affect key proteins survival.

Language: Английский

---

Selective treatment pressure in colon cancer drives the molecular profile of resistant circulating tumor cell clones

Molecular Cancer, Journal Year: 2021, Volume and Issue: 20(1)

Published: Feb. 8, 2021

Abstract The characterization of circulating tumor cells (CTCs) holds promises for precision medicine because these are an important clinical indicator treatment efficacy. We established the first and still only nine permanent colon CTC lines from peripheral blood samples a patient with metastatic cancer collected at different time points during progression. study objectives were ( i ) to compare gene expression profiles lines, ii determine main features acquired treatment. number upregulated genes was higher in obtained after treatment, indicating that they properties escape pressure. Among genes, some involved mTOR PI3K/AKT signaling pathways. Moreover, cytidine deaminase significantly increased failure first- second-line 5-fluorouracile-based treatments, suggesting CTCs can eliminate this specific drug resist therapy. Several enzymes xenobiotic metabolism also activation detoxification mechanisms response chemotherapy. Finally, significant aldolase B four six withdrawal progression indicated clones originated liver metastases. In conclusion, generated single characterized by deregulation promote resistance, energy metabolism, iii stem cell plasticity.

Language: Английский

---

Short-Term Ex Vivo Culture of CTCs from Advance Breast Cancer Patients: Clinical Implications

Cancers, Journal Year: 2021, Volume and Issue: 13(11), P. 2668 - 2668

Published: May 28, 2021

Background: Circulating tumor cells (CTC) have relevance as prognostic markers in breast cancer. However, the functional properties of CTCs or their molecular characterization not been well-studied. Experimental models indicate that only a few can survive circulation and eventually metastasize. Thus, it is essential to identify these surviving capable forming such metastases. Methods: We isolated viable from 50 peripheral blood samples obtained 35 patients with advanced metastatic cancer using RosetteSepTM for ex vivo culture. The were seeded monitored on plates under low adherence conditions media supplemented growth factors Nanoemulsions. Phenotypic analysis was performed by immunofluorescence gene expression RT-PCR counting Cellsearch® system. Results: found 75% CTC cultures lasted more than 23 days, predicting shorter Progression-Free Survival patients, independently having ≥5 Cellsearch®. also observed before after culture showed different profile. Conclusions: cultivability predictive factor. Furthermore, subset growing show stem mesenchymal features may represent population potential vivo.

Language: Английский

---