Mitigation and use of biofilms in space for the benefit of human space exploration DOI Creative Commons

Yo-Ann Vélez Justiniano,

Darla M. Goeres,

Elizabeth L. Sandvik

et al.

Biofilm, Journal Year: 2023, Volume and Issue: 5, P. 100102 - 100102

Published: Jan. 6, 2023

Biofilms are self-organized communities of microorganisms that encased in an extracellular polymeric matrix and often found attached to surfaces. widely present on Earth, diverse sometimes extreme environments. These microbial have been described as recalcitrant or protective when facing adversity environmental exposures. On the International Space Station, biofilms were human-inhabited environments a multitude hardware Moreover, studies identified phenotypic genetic changes under microgravity conditions including microbe surface colonization pathogenicity traits. Lack consistent research microgravity-grown can lead deficient understanding altered behavior space. This could subsequently create problems engineered systems negatively impact human health crewed spaceflights. It is especially relevant long-term remote space missions will lack resupply service. Conversely, also known benefit plant growth essential for (i.e., gut microbiome). Eventually, may be used supply metabolic pathways produce organic inorganic components useful sustaining life celestial bodies beyond Earth. article explore what currently about identify gaps aerospace industry's knowledge should filled order mitigate leverage advantage spaceflight.

Language: Английский

Fitness costs of plasmids carrying antibiotic resistance genes DOI
Yi Xu, Hao Cheng, Xiaojie Hu

et al.

Scientia Sinica Technologica, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Biofilm architecture determines the dissemination of conjugative plasmids DOI Creative Commons

Sarah Djermoun,

Daniel K.H. Rode, Eva Jiménez‐Siebert

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(17)

Published: April 25, 2025

Plasmid conjugation is a contact-dependent horizontal gene transfer mechanism that significantly contributes to the dissemination of antibiotic resistance among bacteria. While molecular mechanisms have been extensively studied, our understanding plasmid dynamics within spatially structured bacterial communities and influence community architecture on remains limited. In this study, we use live-cell fluorescence microscopy investigate propagation broad host range RP4 conjugative in Escherichia coli populations exhibiting varying levels spatial organization. high-density, two-dimensional cell monolayers, direct tight contact between donors recipients not only necessary but also sufficient trigger transfer, ensuring optimal propagation. three-dimensional mature biofilms, emergent limits ability donor cells enter regions with high density, which hinders establishment contacts impedes biofilms. contrast, microcolonies, early-stage biofilms lower surface coverage leave open access points for later emerge as high-cell-density facilitates transfer. These findings reveal crucial role determining efficiency dissemination.

Language: Английский

Citations

0
Quantitative analysis of horizontal gene transfer in complex systems DOI

Jenifer Moralez,

Karolina Szenkiel, Kerry A. Hamilton

et al.

Current Opinion in Microbiology, Journal Year: 2021, Volume and Issue: 62, P. 103 - 109

Published: June 5, 2021

Language: Английский

Citations

22
Growth productivity as a determinant of the inoculum effect for bactericidal antibiotics DOI Creative Commons
Gabriela Diaz-Tang, Estefania Marin Meneses,

Kavish Patel

et al.

Science Advances, Journal Year: 2022, Volume and Issue: 8(50)

Published: Dec. 14, 2022

Understanding the mechanisms by which populations of bacteria resist antibiotics has implications in evolution, microbial ecology, and public health. The inoculum effect (IE), where antibiotic efficacy declines as density a bacterial population increases, been observed for multiple species antibiotics. Several to account IE have proposed, but most lack experimental evidence or cannot explain We show that growth productivity, combined metabolism, can bactericidal species. Guided flux balance analysis whole-genome modeling, we carbon source supplied medium determines productivity. If productivity is sufficiently high, eliminated. Our results may lead approaches reduce clinic, help standardize antibiotics, further our understanding how evolve resistance.

Language: Английский

Citations

15
Mitigation and use of biofilms in space for the benefit of human space exploration DOI Creative Commons

Yo-Ann Vélez Justiniano,

Darla M. Goeres,

Elizabeth L. Sandvik

et al.

Biofilm, Journal Year: 2023, Volume and Issue: 5, P. 100102 - 100102

Published: Jan. 6, 2023

Biofilms are self-organized communities of microorganisms that encased in an extracellular polymeric matrix and often found attached to surfaces. widely present on Earth, diverse sometimes extreme environments. These microbial have been described as recalcitrant or protective when facing adversity environmental exposures. On the International Space Station, biofilms were human-inhabited environments a multitude hardware Moreover, studies identified phenotypic genetic changes under microgravity conditions including microbe surface colonization pathogenicity traits. Lack consistent research microgravity-grown can lead deficient understanding altered behavior space. This could subsequently create problems engineered systems negatively impact human health crewed spaceflights. It is especially relevant long-term remote space missions will lack resupply service. Conversely, also known benefit plant growth essential for (i.e., gut microbiome). Eventually, may be used supply metabolic pathways produce organic inorganic components useful sustaining life celestial bodies beyond Earth. article explore what currently about identify gaps aerospace industry's knowledge should filled order mitigate leverage advantage spaceflight.

Language: Английский

Citations

9