Antibiotic Resistance and Virulence Genes in Escherichia coli Isolated from Patients in a Tertiary Care Hospital: Implications for Clinical Management and Public Health DOI Creative Commons

Ali Ahsan,

Zahra Fatima,

Alina Asif

et al.

BioScientific Review, Journal Year: 2024, Volume and Issue: 6(3), P. 106 - 121

Published: Aug. 28, 2024

Background. Diarrheal diseases, exacerbated by limited access to clean water, remain a significant global health concern. Enterobacteriaceae, particularly Escherichia coli (E. coli), are their prevalent causative agents. The emergence of antibiotic resistance poses grave public threat, with extended spectrum beta-lactamases (ESBL) and carbapenemases contributing significantly. This study aimed identify the antibiogram patterns virulence genes in E. isolates obtained from patients tertiary care hospital. Method. A cross-sectional involving 395 clinical samples hospital Lahore was conducted over period six months. isolation characterization bacterial strains were performed using culture-based, biochemical, morphological assessments. Antibiotic susceptibility testing (AST) carried out Kirby-Bauer (KB) disk diffusion method. DNA extraction molecular identification through PCR. Statistical analysis Excel SPSS. Results. Of samples, found be most (47.6%), followed Klebsiella spp. (43.3%). AST revealed high cefuroxime (85%) ciprofloxacin (80%). Molecular identified traT being (37.2%), fimH aer (28.7%). Notably, sfa, papA, hly, cnf undetected. Conclusion. results showed prevalence factors rates necessitate vigilant antimicrobial stewardship. presence specific emphasizes potential pathogenicity these isolates, underscoring importance effective infection control measures.

Language: Английский

Antibiotic Resistance and Virulence Genes in Escherichia coli Isolated from Patients in a Tertiary Care Hospital: Implications for Clinical Management and Public Health DOI Creative Commons

Ali Ahsan,

Zahra Fatima,

Alina Asif

et al.

BioScientific Review, Journal Year: 2024, Volume and Issue: 6(3), P. 106 - 121

Published: Aug. 28, 2024

Background. Diarrheal diseases, exacerbated by limited access to clean water, remain a significant global health concern. Enterobacteriaceae, particularly Escherichia coli (E. coli), are their prevalent causative agents. The emergence of antibiotic resistance poses grave public threat, with extended spectrum beta-lactamases (ESBL) and carbapenemases contributing significantly. This study aimed identify the antibiogram patterns virulence genes in E. isolates obtained from patients tertiary care hospital. Method. A cross-sectional involving 395 clinical samples hospital Lahore was conducted over period six months. isolation characterization bacterial strains were performed using culture-based, biochemical, morphological assessments. Antibiotic susceptibility testing (AST) carried out Kirby-Bauer (KB) disk diffusion method. DNA extraction molecular identification through PCR. Statistical analysis Excel SPSS. Results. Of samples, found be most (47.6%), followed Klebsiella spp. (43.3%). AST revealed high cefuroxime (85%) ciprofloxacin (80%). Molecular identified traT being (37.2%), fimH aer (28.7%). Notably, sfa, papA, hly, cnf undetected. Conclusion. results showed prevalence factors rates necessitate vigilant antimicrobial stewardship. presence specific emphasizes potential pathogenicity these isolates, underscoring importance effective infection control measures.

Language: Английский

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