Analyses of Long Non-Coding RNA and mRNA profiling using RNA sequencing during the pre-implantation phases in pig endometrium

Scientific Reports, Journal Year: 2016, Volume and Issue: 6(1)

Published: Jan. 29, 2016

Abstract Establishment of implantation in pig is accompanied by a coordinated interaction between the maternal uterine endometrium and conceptus development. We investigated expression profiles endometrial tissue on Days 9, 12 15 pregnancy Day non-pregnancy Yorkshire performed comprehensive analysis long non-coding RNAs (lncRNAs) samples using RNA sequencing. As result, 2805 novel lncRNAs, 2,376 (301 lncRNA 2075 mRNA) differentially expressed genes (DEGs) 2149 transcripts were obtained pairwise comparison. In agreement with previous reports, lncRNAs shared similar characteristics, such as shorter length, lower exon number, at level less conserved than protein coding transcripts. Bioinformatics showed that DEGs involved binding, cellular process, immune system process enriched focal adhesion, Jak-STAT, FoxO MAPK signaling pathway. also found TCONS_01729386 TCONS_01325501 may play vital role embryo pre-implantation. Furthermore, FGF7 , NMB, COL5A3, S100A8 PPP1R3D significantly up-regulated time recognition (Day pregnancy). Our results first identified characterization profile during pre-implantation phases.

Language: Английский

---

The inflammation paradox in the evolution of mammalian pregnancy: turning a foe into a friend

Current Opinion in Genetics & Development, Journal Year: 2017, Volume and Issue: 47, P. 24 - 32

Published: Aug. 30, 2017

Language: Английский

---

Meat science: From proteomics to integrated omics towards system biology

Journal of Proteomics, Journal Year: 2012, Volume and Issue: 78, P. 558 - 577

Published: Nov. 6, 2012

Language: Английский

---

Embryo‐maternal dialogue during pregnancy establishment and implantation in the pig

Molecular Reproduction and Development, Journal Year: 2017, Volume and Issue: 84(9), P. 842 - 855

Published: June 19, 2017

Porcine conceptuses secrete pregnancy‐recognition signals (estrogens, including estradiol‐17β) that inhibit luteolysis, thereby prolonging progesterone production by corpora lutea. The supportive mechanism which the conceptus also inhibits luteolysis is shifting endometrial prostaglandin (PG) synthesis to luteoprotective PGE2. Progesterone stimulates of factors are essential for development. Priming uterus and loss receptors from uterine epithelium D1ay 10–12 after estrus key achieving receptivity implantation. Conceptus implantation involves a series events, many resembling inflammatory reaction, greatly influenced cytokines, growth factors, prostaglandins. We herein present novel, dual role PGF2α in lutea depends on acquisition luteolytic sensitivity, based knowledge triggers pathways involved during estrous cycle or/and may have an alternative function maintaining pregnancy. point out new that, together with PGE2, can act as embryonic signal mediators. PGF2α, until recently was considered undesirable promoting pregnancy, now known stimulate conceptus‐maternal interactions angiogenesis endometrium. This line other important functions, such stimulating adhesion trophoblasts (PGE2, PGI2) well vascular functions trophoblast cell proliferation (PGI2). Finally, microRNAs emerged post‐transcriptional regulators gene function, adding area investigation enhance understanding conceptus‐endometrial interactions.

Language: Английский

---

Inflammation and reproductive function in women with polycystic ovary syndrome

Biology of Reproduction, Journal Year: 2021, Volume and Issue: 104(6), P. 1205 - 1217

Published: March 19, 2021

Polycystic ovary syndrome (PCOS) is one of the most frequent endocrinopathies, affecting 5-10% women reproductive age, and characterized by presence ovarian cysts, oligo, or anovulation, clinical biochemical hyperandrogenism. Metabolic abnormalities such as hyperinsulinemia, insulin resistance, cardiovascular complications, dyslipidemia, obesity are frequently present in PCOS women. Several key pathogenic pathways overlap between these metabolic abnormalities, notably chronic inflammation. The observation that this mechanism was shared led to hypothesis a inflammatory state could contribute pathogenesis PCOS. Moreover, while physiological inflammation an essential feature events ovulation, menstruation, implantation, labor at term, establishment may be pivotal observed dysfunctions Taken together, work aims review available evidence about mediators related mechanisms with PCOS, emphasis on function.

Language: Английский

---

Structure, Function, and Regulation of the Corpus Luteum

Elsevier eBooks, Journal Year: 2014, Volume and Issue: unknown, P. 1023 - 1076

Published: Nov. 21, 2014

Language: Английский

---

Deep Sequencing of the Porcine Endometrial Transcriptome on Day 14 of Pregnancy1

Biology of Reproduction, Journal Year: 2013, Volume and Issue: 88(4)

Published: Feb. 21, 2013

In pigs, conceptus attachment to the uterine surface epithelium starts around Day 14 of pregnancy preceded by a pronounced vascularization at implantation zones, initiating epitheliochorial placentation. To characterize complex transcriptome changes in endometrium course initial attachment, deep sequencing endometrial RNA samples pregnant animals (n = 4) and corresponding cyclic controls was performed using Illumina RNA-Seq. The obtained sequence reads were mapped porcine genome, relative expression values calculated for analysis differential gene expression. Statistical revealed 1933 differentially expressed genes (false discovery rate 1%), 1229 with higher 704 lower mRNA concentration, from animals. Expression selected validated use quantitative real-time RT-PCR. RNA-Seq data compared results microarray study bovine on 18 additional related sets. Bioinformatics concentration strong overrepresentation, particularly immune-related functional terms but also apoptosis cell adhesion. Overrepresented extracellular region, ion transport, adhesion, lipid steroid metabolic process. conclusion, comprehensive differences between highlighted new processes pathways probably involved regulation noninvasive pig.

Language: Английский

---

Transcriptome Changes in the Porcine Endometrium During the Preattachment Phase1

Biology of Reproduction, Journal Year: 2013, Volume and Issue: 89(6)

Published: Oct. 31, 2013

The porcine conceptus undergoes rapid differentiation and expansion of its trophoblastic membranes between Days 11 12 gestation. Concomitant with trophoblast elongation, production estrogen, the embryonic pregnancy recognition signal, increases. Conceptus attachment to uterine surface epithelium starts after Day 13, initiating epitheliochorial placentation. To analyze transcriptome changes in endometrium course maternal pregnancy, deep sequencing endometrial RNA samples pregnant animals (n = 4) corresponding nonpregnant controls was performed using (RNA-Seq). Between 30 000 35 sequence reads per sample were produced mapped genome (Sscrofa10.2). Analysis read counts revealed 2593 differentially expressed genes (DEGs). Expression selected validated by use quantitative real-time RT-PCR. Bioinformatics analysis identified several functional terms specifically overrepresented for up-regulated or down-regulated genes. Comparison RNA-Seq data from 14 at level all genes, DEG, categories. This specific gene expression patterns reflecting different functions during these stages (i.e., preparation attachment). Genes related mitosis, immune response, epithelial cell development, proteolysis, prostaglandin signaling metabolism are discussed detail. study comprehensive associated establishment could be a resource targeted studies pathways potentially involved regulation this process.

Language: Английский

---

Endometrial response to conceptus-derived estrogen and interleukin-1β at the time of implantation in pigs

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2018, Volume and Issue: 9(1)

Published: May 18, 2018

The establishment of pregnancy is a complex process that requires well-coordinated interaction between the implanting conceptus and maternal uterus. In pigs, undergoes dramatic morphological functional changes at time implantation introduces various factors, including estrogens cytokines, interleukin-1β2 (IL1B2), interferon-γ (IFNG), IFN-δ (IFND), into uterine lumen. response to ovarian steroid hormones conceptus-derived endometrium becomes receptive by changing its expression cell adhesion molecules, secretory activity, immune response. Conceptus-derived act as signal for recognition direction prostaglandin (PG) F2α from vasculature Estrogens also induce many endometrial genes, genes related growth synthesis transport PGs, immunity. IL1B2, pro-inflammatory cytokine, produced elongating conceptus. direct effect IL1B2 on function not fully understood. IL1B activates involved in signaling PG transport. addition, estrogen or stimulates IFN suggesting cooperatively priming conceptus-produced IFNG IFND that, turn, modulate during early pregnancy. This review addresses information about maternal-conceptus interactions with respect gene focusing roles pigs.

Language: Английский

---

Behind the Curtain of Abnormal Placentation in Pre-Eclampsia: From Molecular Mechanisms to Histological Hallmarks

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7886 - 7886

Published: July 18, 2024

Successful human pregnancy needs several highly controlled steps to guarantee an oocyte’s fertilization, the embryo’s pre-implantation development, and its subsequent implantation into uterine wall. The placenta development ensures adequate fetal nutrition oxygenation, with trophoblast being first cell lineage differentiate during this process. sustains growth of fetus by providing it oxygen nutrients removing waste products. It is not surprising that issues early can lead common disorders, such as recurrent miscarriage, restriction, pre-eclampsia, stillbirth. Understanding normal essential for recognizing contextualizing any pathological aberrations may occur. effects these become apparent until later in pregnancy, mid or advanced stages. This review discusses process embryo phase, molecular mechanisms involved, abnormalities those are thought contribute pre-eclampsia. also covers histological hallmarks pre-eclampsia found examination placental tissue from patients.

Language: Английский

---