Gene Editing and Systems Biology Tools for Pesticide Bioremediation: A Review

Frontiers in Microbiology, Journal Year: 2019, Volume and Issue: 10

Published: Feb. 13, 2019

Bioremediation is the degradation potential of microorganisms to dissimilate complex chemical compounds from surrounding environment. The genetics and biochemistry biodegradation processes in datasets opened way systems biology. Systemic biology aid study interacting parts involved system. significant keys system are network, computational biology, omics approaches. Biodegradation network consists all databases which assisting deterioration for bioremediation processes. This review deciphers bio-degradation i.e., (UM-BBD, PAN, PTID etc.) aiding processes, multi approaches like metagenomics, genomics, transcriptomics, proteomics metabolomics efficient functional gene mining their validation experiments. Besides, present also describes editing tools CRISPR Cas, TALEN ZFNs can possibly make design microbe with interest particular recalcitrant improved bioremediation.

Language: Английский

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An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms

eLife, Journal Year: 2019, Volume and Issue: 8

Published: Nov. 1, 2019

We previously reported a CRISPR-mediated knock-in strategy into introns of

Language: Английский

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Microbial Biodiversity and Bioremediation Assessment Through Omics Approaches

Frontiers in Environmental Chemistry, Journal Year: 2020, Volume and Issue: 1

Published: Sept. 25, 2020

Industrialization, population burst, and changing lifestyles have resulted in the genesis of non-degradable pollutants languishing environment human health. Biological approaches using microorganisms are gaining importance as an eco-friendly cost-effective substitute to mitigate pollution load. Microorganisms can survive a divergent produce metabolites that degrade transform making it possible revive contaminated sites naturally. Modern omics technologies like metagenomics, transcriptomics, proteomics, etc. been used nowadays design strategies study ecology diversity their application environmental monitoring bioremediation. The present article will focus on techniques reportedly tackle

Language: Английский

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The wing imaginal disc

Genetics, Journal Year: 2022, Volume and Issue: 220(4)

Published: March 4, 2022

Abstract The Drosophila wing imaginal disc is a tissue of undifferentiated cells that are precursors the and most notum adult fly. first forms during embryogenesis from cluster ∼30 located in second thoracic segment, which invaginate to form sac-like structure. They undergo extensive proliferation larval stages mature ∼35,000 cells. During this time, distinct cell fates assigned different regions, develops complex morphology. Finally, pupal undergoes morphogenetic processes then differentiates notum. While bulk comprises epithelial cells, it also includes neurons glia, associated with tracheal muscle precursor relative simplicity accessibility disc, combined wealth genetic tools available Drosophila, have make premier system for identifying genes deciphering systems play crucial roles animal development. Studies discs made key contributions many areas biology, including patterning, signal transduction, growth control, regeneration, planar polarity, morphogenesis, mechanics.

Language: Английский

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Two-Faced: Roles of JNK Signalling During Tumourigenesis in the Drosophila Model

Frontiers in Cell and Developmental Biology, Journal Year: 2020, Volume and Issue: 8

Published: Feb. 5, 2020

The highly conserved c-Jun N-terminal Kinase (JNK) signalling pathway has many functions, regulating a diversity of processes: from cell movement during embryogenesis to the stress response cells after environmental insults. Studies modelling cancer using vinegar fly, Drosophila melanogaster, have identified both pro- and anti-tumourigenic roles for JNK signalling, depending on context. As tumour suppressor, commonly is activated by Tumour Necrosis Factor (TNF) which promotes caspase-mediated death tumourigenic cells. activation can also occur via actin cytoskeleton alterations, cellular damage inflicted reactive oxygen species (ROS). Additionally, frequently acts in concert with Salvador-Warts-Hippo (SWH) – either upstream or parallel this potent growth-suppressing pathway. promoter, co-opted expressing Ras-MAPK (among other pathways), used drive morphological changes, induce invasive behaviours, block differentiation, enable persistent proliferation. Furthermore, capable non-autonomous influences within microenvironments effecting transcription various growth- proliferation-promoting molecules. In review, we discuss these aspects tumourigenesis models, highlight recent publications that expanded our knowledge powerful

Language: Английский

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Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: May 8, 2019

Abstract Gene editing by CRISPR/Cas9 is commonly used to generate germline mutations or perform in vitro screens, but applicability for vivo screening has so far been limited. Recently, it was shown that Drosophila , Cas9 expression could be limited a desired group of cells, allowing tissue-specific mutagenesis. Here, we thoroughly characterize (ts)CRISPR within the complex neuronal system mushroom body. We report generation library gRNA-expressing plasmids and fly lines using optimized tools, which provides valuable resource community. demonstrate application our large-scale screen, reveals insights into developmental remodeling.

Language: Английский

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Precise genome engineering in Drosophila using prime editing

Proceedings of the National Academy of Sciences, Journal Year: 2020, Volume and Issue: 118(1)

Published: Dec. 21, 2020

Precise genome editing is a valuable tool to study gene function in model organisms. Prime editing, precise system developed mammalian cells, does not require double-strand breaks or donor DNA and has low off-target effects. Here, we applied prime for the organism

Language: Английский

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An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR-mediated homologous recombination

eLife, Journal Year: 2022, Volume and Issue: 11

Published: June 20, 2022

Previously, we described a large collection of

Language: Английский

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Kinetic principles underlying pioneer function of GAGA transcription factor in live cells

Nature Structural & Molecular Biology, Journal Year: 2022, Volume and Issue: 29(7), P. 665 - 676

Published: July 1, 2022

Language: Английский

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Deciphering the recent trends in pesticide bioremediation using genome editing and multi-omics approaches: a review

World Journal of Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 39(6)

Published: April 8, 2023

Language: Английский

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