Insights into regeneration tool box: An animal model approach

Developmental Biology, Journal Year: 2019, Volume and Issue: 453(2), P. 111 - 129

Published: April 13, 2019

Language: Английский

---

Recurrent gene co-amplification on Drosophila X and Y chromosomes

PLoS Genetics, Journal Year: 2019, Volume and Issue: 15(7), P. e1008251 - e1008251

Published: July 22, 2019

Y chromosomes often contain amplified genes which can increase dosage of male fertility and counteract degeneration via gene conversion. Here we identify with increased copy number on both X in various species Drosophila, a pattern that has previously been associated sex chromosome drive involving the Slx Sly families mice. We show recurrent X/Y co-amplification appears to be an important evolutionary force shaped content evolution Drosophila. demonstrate convergent acquisition amplification testis expressed are common Drosophila chromosomes, especially recently formed ones, carefully characterize one putative novel system. find S-Lap1/GAPsec pair occurred independently several times members D. obscura group, where this normally autosomal is sex-linked due chromosome-autosome fusion. explore scenarios would explain co-amplification. Investigation expression short RNA profiles at system suggest that, like Slx/Sly mice, these may remnants cryptic system, however additional transgenic experiments will necessary validate model. Regardless whether responsible for co-amplification, our findings duplications between could have widespread effect genomic patterns, including epigenetic regulation distribution sex-biased genes, hybrid sterility.

Language: Английский

---

DROP: Molecular voucher database for identification of Drosophila parasitoids

Molecular Ecology Resources, Journal Year: 2021, Volume and Issue: 21(7), P. 2437 - 2454

Published: June 4, 2021

Molecular identification is increasingly used to speed up biodiversity surveys and laboratory experiments. However, many groups of organisms cannot be reliably identified using standard databases such as GenBank or BOLD due lack sequenced voucher specimens by experts. Sometimes a large number sequences are available, but with too errors allow identification. Here, we address this problem for parasitoids Drosophila introducing curated open-access molecular reference database, DROP (Drosophila parasitoids). Identifying challenging poses major impediment realize the full potential model system in studies ranging from mechanisms food webs, biological control suzukii. In DROP, genetic data linked and, where possible, taxonomists vetted through direct comparison primary type material. To initiate 154 strains, 856 vouchers, 554 DNA sequences, 16 genomes, 14 transcriptomes, six proteomes drawn total 183 operational taxonomic units (OTUs): 114 described parasitoid species 69 provisional species. We found richness heavily underestimated provide an updated catalogue community. offers accurate improves cross-referencing between individual that hope will catalyse research on diverse fascinating system. Our effort should also serve example researchers facing similar problems other organisms.

Language: Английский

---

Insect Radiations on Islands: Biogeographic Pattern and Evolutionary Process in Hawaiian Insects

The Quarterly Review of Biology, Journal Year: 2021, Volume and Issue: 96(4), P. 247 - 296

Published: Dec. 1, 2021

The spectacular adaptive radiations on the Hawaiian Islands offer tantalizing possibility of ascertaining predictability evolution, especially with regard to how archipelago's dynamic geology suggests a priori hypotheses for evolutionary pattern and process. In past two decades advances in sequencing phylogenetics have shed new light evolution this model fauna. Here, we provide an overview recent research major endemic insect radiations. We find that, contrast earlier views, substantial fraction clades colonized archipelago prior formation current high islands (5.1 Ma) persisted through one or more rounds island subsidence emergence archipelago. Many show elements progression rule, null expectation where biogeographic patterns phylogeny mirror chronological sequence formation, but few match its predictions exactly. Diversity most is shaped by complicated interplay among-island dispersal within-island diversification. Finally, positive relationship between clade age species richness insects, great deal variance that likely explained aspects ecology clade-specific factors.

Language: Английский

---

Genome-powered classification of microbial eukaryotes: focus on coral algal symbionts

Trends in Microbiology, Journal Year: 2022, Volume and Issue: 30(9), P. 831 - 840

Published: Feb. 25, 2022

Language: Английский

---

Comparative analysis of temperature preference behavior and effects of temperature on daily behavior in 11 Drosophila species

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: July 25, 2022

Abstract Temperature is one of the most critical environmental factors that influence various biological processes. Species distributed in different temperature regions are considered to have optimal temperatures for daily life activities. However, how organisms acquired features cope with particular environments remains be elucidated. In this study, we systematically analyzed preference behavior and effects on locomotor activity sleep using 11 Drosophila species. We also investigated function antennae these found that, (1) an each species approximately matches it frequently encounters its habitat, (2) diverse among species, but maintains pattern even at temperatures, (3) has a unique behavior, contribution These results suggest inhabiting climatic species-specific response systems according their strategies. This study provides fundamental information understanding mechanisms underlying adaptation lifestyle diversification.

Language: Английский

---

A connectomics-based taxonomy of mammals

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Nov. 7, 2022

Mammalian taxonomies are conventionally defined by morphological traits and genetics. How species differ in terms of neural circuits whether inter-species differences circuit organization conform to these is unknown. The main obstacle the comparison architectures has been network reconstruction techniques, yielding species-specific connectomes that not directly comparable one another. Here, we comprehensively chart connectome across mammalian phylogenetic spectrum using a common protocol. We analyse MRI (MaMI) data set, database encompasses high-resolution ex vivo structural diffusion scans 124 12 taxonomic orders 5 superorders, collected unified assess similarity between two methods: Laplacian eigenspectra multiscale topological features. find greater similarities among within same order, suggesting reflects established relationships morphology While all retain hallmark global features relative proportions connection classes, variation driven local regional connectivity profiles. By encoding into frame reference, findings establish foundation for investigating how change over phylogeny, forging link from genes behaviour.

Language: Английский

---

Single-fly assemblies fill major phylogenomic gaps across the Drosophilidae Tree of Life

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 2, 2023

Abstract Long-read sequencing is driving rapid progress in genome assembly across all major groups of life, including species the family Drosophilidae, a longtime model system for genetics, genomics, and evolution. We previously developed cost-effective hybrid Oxford Nanopore (ONT) long-read Illumina short-read approach used it to assemble 101 drosophilid genomes from laboratory cultures, greatly increasing number assemblies this taxonomic group. The next challenge address culture bias taxon sampling by that cannot easily be reared lab. Here, we build upon our previous methods perform amplification-free ONT single wild flies obtained either directly field or ethanol-preserved specimens museum collections, improving representation lesser studied taxa whole-genome data. Using Novaseq X Plus P2 sequencers with R10.4.1 chemistry, set new benchmark inexpensive at US $150 per while assembling as little 35 ng genomic DNA fly. present 183 179 resource systematics, phylogenetics, comparative genomics. Of these genomes, 62 are pooled lab strains 121 adult flies. Despite sample limitations working small insects, most single-fly diploid comparable contiguity (>1Mb contig N50), completeness (>98% complete dipteran BUSCOs), accuracy (>QV40 genome-wide R10.4.1) inbred lines. well-resolved multi-locus phylogeny 360 4 outgroup encompassing publicly available (as August 2023) Finally, Progressive Cactus whole-genome, reference-free alignment built subset 298 suitably high-quality genomes. alignment, along updated protocols computational pipelines, released an open tool studying evolution scale entire insect family.

Language: Английский

---

Linking neural circuits to the mechanics of animal behavior in Drosophila larval locomotion

Frontiers in Neural Circuits, Journal Year: 2023, Volume and Issue: 17

Published: Aug. 17, 2023

The motions that make up animal behavior arise from the interplay between neural circuits and mechanical parts of body. Therefore, in order to comprehend operational mechanisms governing behavior, it is essential examine not only underlying network but also characteristics animal’s locomotor system fly larvae serves as an ideal model for pursuing this integrative approach. By virtue diverse investigation methods encompassing connectomics analysis quantification locomotion kinematics, research on larval has shed light behavior. These studies have elucidated roles interneurons coordinating muscle activities within segments, well responsible exploration. This review aims provide overview recent neuromechanics larvae. We briefly interspecific diversity explore latest advancements soft robots inspired by locomotion. using could establish a practical framework scrutinizing other species.

Language: Английский

---

Preference for and resistance to a toxic sulfur volatile opens up a unique niche in Drosophila busckii

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 17, 2025

The ability to tolerate otherwise toxic compounds can open up unique niches in nature. Among drosophilid flies, few examples of such adaptations are known and those which typically from highly host-specific species. Here we show that the human commensal species Drosophila busckii uses dimethyldisulfide (DMDS) as a key mediator its host selection. Despite DMDS's neurotoxic properties, D. has evolved tolerance towards high concentrations compound an olfactory cue pinpoint food oviposition sites. This adaptability is likely linked insensitivity enzyme complex cytochrome c oxidase (COX), DMDS target other insects. Our findings position potential model for studying resistance gases affecting COX offers insight into evolutionary within specific ecological contexts.

Language: Английский

---