Use of dimethylformamide to cryopreserve alpaca semen previously incubated with collagenase DOI
Nils Herber Flores Huarco, S. Giuliano, Fernanda Gabriela Fumuso

et al.

Reproduction in Domestic Animals, Journal Year: 2021, Volume and Issue: 56(11), P. 1387 - 1397

Published: Aug. 11, 2021

Abstract The objective of this study was to evaluate the effect collagenase and two final dimethylformamide (DMF) concentrations (4% 7%) on alpaca frozen‐thawed sperm quality. A total 25 ejaculates from 5 were obtained using electroejaculation. Each individual ejaculate evaluated then diluted 4:1 in a solution 1 mg/ml HEPES‐TALP medium incubated for 4 min at 37°C. Subsequently, samples TRIS‐fructose‐citric acid‐egg yolk cooled 5°C. Then, each sample divided aliquots DMF concentration 4% or 7% added, equilibrated hr 5°C frozen over liquid nitrogen vapours. Kruskal–Wallis test used morphometry, Completely Random Block designs analyse motility, viability, membrane function acrosome status. After incubation, none showed thread formation, parameters preserved. Non‐progressive motile higher ( p < .05) DMF: 31.8 ± 8.3% 36.3 11.8%) compared raw (10.1 4.3%) semen 9.7 1.8% 7.5 3.2%). Sperm function, integrity intact acrosomes (40.1 12.2%, 94.6 3.2% 91.3 8.1%) 19.8 4.7%, 53.2 2.7%, 65.7 8.7% 20.4 4.5%, 54.1 1.4%, 64.6 9.1%). Length head lower samples, being statistically different with pre‐freezing samples. ratio between areas greater Incubation decreased formation without affecting Frozen treated did not preserve thawed

Language: Английский

Use of dimethylformamide to cryopreserve alpaca semen previously incubated with collagenase DOI
Nils Herber Flores Huarco, S. Giuliano, Fernanda Gabriela Fumuso

et al.

Reproduction in Domestic Animals, Journal Year: 2021, Volume and Issue: 56(11), P. 1387 - 1397

Published: Aug. 11, 2021

Abstract The objective of this study was to evaluate the effect collagenase and two final dimethylformamide (DMF) concentrations (4% 7%) on alpaca frozen‐thawed sperm quality. A total 25 ejaculates from 5 were obtained using electroejaculation. Each individual ejaculate evaluated then diluted 4:1 in a solution 1 mg/ml HEPES‐TALP medium incubated for 4 min at 37°C. Subsequently, samples TRIS‐fructose‐citric acid‐egg yolk cooled 5°C. Then, each sample divided aliquots DMF concentration 4% or 7% added, equilibrated hr 5°C frozen over liquid nitrogen vapours. Kruskal–Wallis test used morphometry, Completely Random Block designs analyse motility, viability, membrane function acrosome status. After incubation, none showed thread formation, parameters preserved. Non‐progressive motile higher ( p < .05) DMF: 31.8 ± 8.3% 36.3 11.8%) compared raw (10.1 4.3%) semen 9.7 1.8% 7.5 3.2%). Sperm function, integrity intact acrosomes (40.1 12.2%, 94.6 3.2% 91.3 8.1%) 19.8 4.7%, 53.2 2.7%, 65.7 8.7% 20.4 4.5%, 54.1 1.4%, 64.6 9.1%). Length head lower samples, being statistically different with pre‐freezing samples. ratio between areas greater Incubation decreased formation without affecting Frozen treated did not preserve thawed

Language: Английский

Citations

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