The National Wastewater Surveillance System (NWSS): From inception to widespread coverage, 2020–2022, United States
Carly Adams,
No information about this author
Megan Bias,
No information about this author
Rory M. Welsh
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et al.
The Science of The Total Environment,
Journal Year:
2024,
Volume and Issue:
924, P. 171566 - 171566
Published: March 9, 2024
Wastewater
surveillance
is
a
valuable
tool
that
can
be
used
to
track
infectious
diseases
in
community.
In
September
2020,
the
Centers
for
Disease
Control
and
Prevention
(CDC)
established
National
Surveillance
System
(NWSS)
coordinate
build
nation's
capacity
detect
quantify
concentrations
of
SARS-CoV-2
RNA
U.S.
wastewater.
This
first
summary
NWSS,
covering
1,
2020
December
31,
2022.
Through
partnerships
with
state,
tribal,
local,
territorial
health
departments,
NWSS
became
national
platform
readily
expanded
adapted
meet
changing
public
needs.
Beginning
209
sampling
sites
rapidly
>1500
by
2022,
≈47
%
population.
As
>152,000
unique
wastewater
samples
have
been
collected
partners,
primarily
from
treatment
plants
(WWTPs).
WWTPs
participating
tend
larger
than
average
WWTP
serve
more
populated
communities.
≈8
nearly
16,000
were
NWSS.
partners
variety
methods
testing
samples;
however,
progress
being
made
standardize
these
methods.
July
2021,
started
submitting
genome
sequencing
data
October
monkeypox
virus
testing,
plans
include
additional
disease
targets
future.
rapid
implementation
expansion
important
lessons
learned.
programs
should
consider
both
surge
long-term
capacities
when
developing
an
plan,
early
standardization
facilitate
comparisons
across
sites.
has
proven
flexible
sustainable
system
will
continue
useful
complement
case-based
guiding
action.
Language: Английский
Solid–liquid partitioning of dengue, West Nile, Zika, hepatitis A, influenza A, and SARS-CoV-2 viruses in wastewater from across the USA
Environmental Science Water Research & Technology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 1, 2024
Limited
information
is
available
on
the
fate
of
respiratory
and
arthropod-borne
viruses
in
wastewater.
Language: Английский
Challenges and Opportunities for Wastewater Monitoring of Influenza Viruses During the Multistate Outbreak of Highly Pathogenic Avian Influenza A(H5N1) Virus in Dairy Cattle and Poultry
American Journal of Public Health,
Journal Year:
2024,
Volume and Issue:
unknown, P. e1 - e4
Published: Sept. 19, 2024
Language: Английский
Australian and New Zealand Laboratory Experience and Proposed Future Direction of Wastewater Pathogen Genomic Surveillance
Avram Levy,
No information about this author
Christina Crachi,
No information about this author
Jake Gazeley
No information about this author
et al.
Environments,
Journal Year:
2025,
Volume and Issue:
12(4), P. 114 - 114
Published: April 8, 2025
Wastewater
pathogen
surveillance
was
rapidly
implemented
across
Australia
and
New
Zealand
as
a
public
health
tool
during
the
COVID-19
pandemic.
To
assess
method
consistency
identify
opportunities
for
harmonization,
we
surveyed
all
Australian
Laboratories
conducting
government-funded
wastewater
surveillance.
The
survey
demonstrated
alignment
of
some
choices,
particularly
municipal
treatment
plant
(WWTP)
sampling
use
electromagnetic
membrane
filtration
followed
by
RT-qPCR.
However,
key
differences
were
observed
in
sample
volumes;
nucleic
acid
purification
methods;
validation
approaches;
sequencing,
analysis,
reporting
methods
SARS-CoV-2
lineages.
A
lack
consensus
on
best-practice
evident,
highlighting
need
interlaboratory
data
exchanges
to
support
comparability.
Following
pandemic
period,
several
jurisdictional
programs
discontinued
despite
mounting
international
evidence
utility
wastewater-based
epidemiology
range
pathogens.
Subsequently,
nationally
funded
program
announced
Australia,
necessitating
re-establishment
laboratory
capacity
jurisdictions
expansion
target
pathogens
other
centers.
results
this
are
intended
inform
enhancement
regional
provide
foundation
knowledge
sharing
approach
harmonization.
Language: Английский
Title: Qualitative Assessment of a Novel Results-Based Partnership between National Wastewater Surveillance Centers of Excellence and Utility Companies, Houston (Texas), Colorado, Wisconsin, and California, 2023.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 21, 2024
Abstract
Background
The
U.S.
Centers
for
Disease
Control
and
Prevention
(CDC)
initiated
the
National
Wastewater
Surveillance
System
(NWSS)
in
September
2020.
Four
of
Excellence
(COEs)
were
established
between
2021–2023
Houston
(Texas),
Colorado,
Wisconsin,
California
to
guide
wastewater
surveillance
efforts
public
health.
Our
objective
was
increase
understanding
factors
that
facilitated
implementation
from
perspectives
experiences
shared
by
health
department
COEs
utility
partners.
Methods
We
used
a
purposive
sampling
strategy
select
eight
key
respondents
four
NWSS
respective
conducted
in-depth
interviews
related
identified
common
points
interview
transcription
files.
Insights
on
WWS
partners
distilled
responses
into
lessons
learned.
Results
Three
primary
themes
emerged
after
we
analyzed
responses:
perceived
community
benefits
surveillance,
collaboration
trust
building
among
helped
program
advancement,
sustainability
strategies
considerations.
Conclusion
This
analysis
has
highlighted
importance
partnerships
collaborations.
Language: Английский
Solid-liquid partitioning of Dengue, West Nile, Zika, Hepatitis A, Influenza A, and SARS-CoV-2 viruses in wastewater from across the United States
Published: April 3, 2024
Limited
information
is
available
on
the
fate
of
respiratory
and
arthropod-borne
viruses
in
wastewater.
Enteric
have
been
extensively
studied
wastewater
treatment
plants,
however
partition
coefficients
not
well
documented.
This
essential
for
interpreting
wastewater-based
surveillance
(WBS)
data
optimizing
sample
collection
processing
methods.
In
this
study,
we
examined
solid-liquid
partitioning
behavior
Dengue,
West
Nile,
Zika,
Hepatitis
A,
Influenza
SARS-CoV-2
Samples
were
collected
from
primary
sludge
line
eleven
plants
across
United
States
spiked
with
varying
concentrations
each
virus.
Solid
liquid
fractions
separated
via
centrifugation.
Viral
nucleic
acids
extracted
quantified
using
reverse-transcription
digital
droplet
PCR
(RT-ddPCR).
Partition
(KF),
determined
Freundlich
adsorption
model,
ranged
4.0✕10^2
mL/g
to
3.9✕10^6
(median
=
1.1✕10^4
mL/g).
KF
values
significantly
different
between
viruses.
However,
significant
differences
observed
Zika
Nile
Virus
plants.
Further
research
needed
understand
how
characteristics
might
impact
viral
markers.
The
results
experiment
underscore
importance
considering
solids
early
detection
monitoring
infectious
diseases,
particularly
regions
low
prevalence
infections.
Language: Английский