Solid-liquid partitioning of Dengue, West Nile, Zika, Hepatitis A, Influenza A, and SARS-CoV-2 viruses in wastewater from across the United States DOI Open Access
Laura Roldan-Hernandez, Camila Van Oost, Alexandria B. Boehm

et al.

Published: April 3, 2024

Limited information is available on the fate of respiratory and arthropod-borne viruses in wastewater. Enteric have been extensively studied wastewater treatment plants, however partition coefficients not well documented. This essential for interpreting wastewater-based surveillance (WBS) data optimizing sample collection processing methods. In this study, we examined solid-liquid partitioning behavior Dengue, West Nile, Zika, Hepatitis A, Influenza SARS-CoV-2 Samples were collected from primary sludge line eleven plants across United States spiked with varying concentrations each virus. Solid liquid fractions separated via centrifugation. Viral nucleic acids extracted quantified using reverse-transcription digital droplet PCR (RT-ddPCR). Partition (KF), determined Freundlich adsorption model, ranged 4.0✕10^2 mL/g to 3.9✕10^6 (median = 1.1✕10^4 mL/g). KF values significantly different between viruses. However, significant differences observed Zika Nile Virus plants. Further research needed understand how characteristics might impact viral markers. The results experiment underscore importance considering solids early detection monitoring infectious diseases, particularly regions low prevalence infections.

Language: Английский

The National Wastewater Surveillance System (NWSS): From inception to widespread coverage, 2020–2022, United States DOI Creative Commons
Carly Adams,

Megan Bias,

Rory M. Welsh

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 924, P. 171566 - 171566

Published: March 9, 2024

Wastewater surveillance is a valuable tool that can be used to track infectious diseases in community. In September 2020, the Centers for Disease Control and Prevention (CDC) established National Surveillance System (NWSS) coordinate build nation's capacity detect quantify concentrations of SARS-CoV-2 RNA U.S. wastewater. This first summary NWSS, covering 1, 2020 December 31, 2022. Through partnerships with state, tribal, local, territorial health departments, NWSS became national platform readily expanded adapted meet changing public needs. Beginning 209 sampling sites rapidly >1500 by 2022, ≈47 % population. As >152,000 unique wastewater samples have been collected partners, primarily from treatment plants (WWTPs). WWTPs participating tend larger than average WWTP serve more populated communities. ≈8 nearly 16,000 were NWSS. partners variety methods testing samples; however, progress being made standardize these methods. July 2021, started submitting genome sequencing data October monkeypox virus testing, plans include additional disease targets future. rapid implementation expansion important lessons learned. programs should consider both surge long-term capacities when developing an plan, early standardization facilitate comparisons across sites. has proven flexible sustainable system will continue useful complement case-based guiding action.

Language: Английский

Citations

25
Solid–liquid partitioning of dengue, West Nile, Zika, hepatitis A, influenza A, and SARS-CoV-2 viruses in wastewater from across the USA DOI Creative Commons
Laura Roldan-Hernandez, Camila Van Oost, Alexandria B. Boehm

et al.

Environmental Science Water Research & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Limited information is available on the fate of respiratory and arthropod-borne viruses in wastewater.

Language: Английский

Citations

13
Challenges and Opportunities for Wastewater Monitoring of Influenza Viruses During the Multistate Outbreak of Highly Pathogenic Avian Influenza A(H5N1) Virus in Dairy Cattle and Poultry DOI
Margaret A. Honein, Sonja J. Olsen, Daniel B. Jernigan

et al.

American Journal of Public Health, Journal Year: 2024, Volume and Issue: unknown, P. e1 - e4

Published: Sept. 19, 2024

Language: Английский

Citations

4
Australian and New Zealand Laboratory Experience and Proposed Future Direction of Wastewater Pathogen Genomic Surveillance DOI Open Access
Avram Levy,

Christina Crachi,

Jake Gazeley

et al.

Environments, Journal Year: 2025, Volume and Issue: 12(4), P. 114 - 114

Published: April 8, 2025

Wastewater pathogen surveillance was rapidly implemented across Australia and New Zealand as a public health tool during the COVID-19 pandemic. To assess method consistency identify opportunities for harmonization, we surveyed all Australian Laboratories conducting government-funded wastewater surveillance. The survey demonstrated alignment of some choices, particularly municipal treatment plant (WWTP) sampling use electromagnetic membrane filtration followed by RT-qPCR. However, key differences were observed in sample volumes; nucleic acid purification methods; validation approaches; sequencing, analysis, reporting methods SARS-CoV-2 lineages. A lack consensus on best-practice evident, highlighting need interlaboratory data exchanges to support comparability. Following pandemic period, several jurisdictional programs discontinued despite mounting international evidence utility wastewater-based epidemiology range pathogens. Subsequently, nationally funded program announced Australia, necessitating re-establishment laboratory capacity jurisdictions expansion target pathogens other centers. results this are intended inform enhancement regional provide foundation knowledge sharing approach harmonization.

Language: Английский

Citations

0
Title: Qualitative Assessment of a Novel Results-Based Partnership between National Wastewater Surveillance Centers of Excellence and Utility Companies, Houston (Texas), Colorado, Wisconsin, and California, 2023. DOI Creative Commons
Hannah Turner, Libby Horter, Michael Welton

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Abstract Background The U.S. Centers for Disease Control and Prevention (CDC) initiated the National Wastewater Surveillance System (NWSS) in September 2020. Four of Excellence (COEs) were established between 2021–2023 Houston (Texas), Colorado, Wisconsin, California to guide wastewater surveillance efforts public health. Our objective was increase understanding factors that facilitated implementation from perspectives experiences shared by health department COEs utility partners. Methods We used a purposive sampling strategy select eight key respondents four NWSS respective conducted in-depth interviews related identified common points interview transcription files. Insights on WWS partners distilled responses into lessons learned. Results Three primary themes emerged after we analyzed responses: perceived community benefits surveillance, collaboration trust building among helped program advancement, sustainability strategies considerations. Conclusion This analysis has highlighted importance partnerships collaborations.

Language: Английский

Citations

1
Solid-liquid partitioning of Dengue, West Nile, Zika, Hepatitis A, Influenza A, and SARS-CoV-2 viruses in wastewater from across the United States DOI Open Access
Laura Roldan-Hernandez, Camila Van Oost, Alexandria B. Boehm

et al.

Published: April 3, 2024

Limited information is available on the fate of respiratory and arthropod-borne viruses in wastewater. Enteric have been extensively studied wastewater treatment plants, however partition coefficients not well documented. This essential for interpreting wastewater-based surveillance (WBS) data optimizing sample collection processing methods. In this study, we examined solid-liquid partitioning behavior Dengue, West Nile, Zika, Hepatitis A, Influenza SARS-CoV-2 Samples were collected from primary sludge line eleven plants across United States spiked with varying concentrations each virus. Solid liquid fractions separated via centrifugation. Viral nucleic acids extracted quantified using reverse-transcription digital droplet PCR (RT-ddPCR). Partition (KF), determined Freundlich adsorption model, ranged 4.0✕10^2 mL/g to 3.9✕10^6 (median = 1.1✕10^4 mL/g). KF values significantly different between viruses. However, significant differences observed Zika Nile Virus plants. Further research needed understand how characteristics might impact viral markers. The results experiment underscore importance considering solids early detection monitoring infectious diseases, particularly regions low prevalence infections.

Language: Английский

Citations

0