A multi-antigen Campylobacter vaccine enhances antibody responses in layer breeders and sustains elevated maternal antibody levels in their offspring

Poultry Science, Journal Year: 2025, Volume and Issue: 104(4), P. 104898 - 104898

Published: Feb. 17, 2025

Campylobacter jejuni is the leading cause of bacterial gastroenteritis worldwide, with an estimated 1.5 million human infections occurring annually in United States alone. With chickens being considered primary reservoir and source infection humans, developing effective vaccination strategies crucial for preventing transmission to humans. This study aimed examine immunogenicity protective efficacy a multi-antigen subunit vaccine, consisting C. outer membrane proteins (OMPs) cytosine-phosphorothioate-guanine oligodeoxynucleotides (CpG ODN), layer breeders its potential enhance levels jejuni-specific maternal antibodies their offspring. Four groups were subcutaneously vaccinated 200 μg OMPs 50 CpG-ODN, individually or combined, PBS as negative control. shedding antibody monitored up ten weeks post-vaccination. At peak (the fourth week post-primary vaccination), fertilized eggs collected incubated sensitized egg incubator until hatching. Maternally derived (MDA) measured serum hatched chicks five post-hatch. The results revealed that combination CpG ODN exhibited significant reduction by 1.37 log10. group also showed significantly higher yolk IgY IgM compared non-vaccinated control group, notably, maintained IgY, IgM, IgA post-hatch group. Overall, these outcomes suggest could offer promising vaccine strategy reduce colonization boost sustain high MDA chicks. Further research needed evaluate effects vaccine-associated against commercial broiler model.

Language: Английский

Immunoprotection of Broiler Chickens Against Necrotic Enteritis by Oral Delivery of a Live Clostridium perfringens Vaccine Adjuvanted with Cholera Toxin at Hatch

Avian Diseases, Journal Year: 2025, Volume and Issue: 69(1)

Published: March 13, 2025

Language: Английский