Comparative Characteristics of Corneal Confocal Microscopy after UV-A Cross-linking with Different Riboflavin Solutions in Experiment

Ophthalmology in Russia, Journal Year: 2024, Volume and Issue: 21(4), P. 802 - 808

Published: Dec. 24, 2024

Corneal cross-linking (CXL) has become the most common way of managing keratectasia. To date, Dresden protocol (G. Wollensak et al., 2003) acquired many modifications, in which composition applied agents or modes CXL procedure have been changed. This article presents results studying morphological changes rabbit cornea vivo after using riboflavin solutions different composition. Purpose : compare effect on corneal structure experimental animals by confocal microscopy with saline, dextran and hydroxypropylmethylcellulose. Material methods . The study was performed 20 eyes 15 rabbits, underwent standard (Epi-Off) 0.1 % 3 groups: first group saline used as a base, second — dextran, third 1.0 hydroxypropyl methylcellulose (HPMC). irradiation mode: mW/cm2, 30 minutes, wavelength 370 nm. condition evaluated at 3, 7, 14, 90 days CXL. Biomicroscopy were all Heidelberg Retinal Tomographer HRT-III (Heidelberg Engineering, Germany). Keratocyte density counted depth 120–160 μm central zone area 1 mm2. Statistical processing Statistica 6.1 Excel 2010 programmes. Results In biomicroscopy corneas showed postoperative epithelium stroma groups, 7 CXL, there decrease keratocyte density, due to apoptosis, observed predominantly anterior layers stroma. number keratocytes 192.5 ± 29.8 cells/mm2, 2 227.4 38.2 204.4 32.6 cells/mm2 against intact control 352.8 35.2 cells/mm2. There not statistically significant difference between groups. Conclusion lifetime demonstrated similar early period, accompanied loss subepithelial nerve fibres, development lacunar edema, apoptosis their mainly middle beginning repopulation fibre regeneration 30th day No signs endothelium damage detected. A comparative assessment greatest effectiveness techniques 0.1% HPMC can be given basis larger-scale clinical studies.

Language: Английский

Corneal crosslinking in septic melting corneal ulcers in dogs and cats

Russian veterinary journal, Journal Year: 2019, Volume and Issue: 2019(1), P. 11 - 17

Published: March 18, 2019

Objective of research. To evaluate the efficacy and safety corneal collagen cross-linking during melting ulcers in dogs cats. Describe changes properties structure cornea entire observation period. Materials methods. 15 animals were included study: 8 7 cats that received preliminary treatment for underlying disease, but without positive dynamics. All had an infectious-inflammatory process with signs keratomalacia. underwent mechanical diepithelization simultaneous surface surgical pathological tissues. CXL procedure was performed under general anesthesia saturation stroma riboflavin solution 30 minutes. Results. showed a decrease purulent-inflammatory 3...5 days after CXL. Keratomalacia inhibition noted on average by 2 ... 3 procedure. By 7th day observation, stabilization wound epithelization defect observed 11 animals. In 4 animals, this lasted up to 10...12 days. 13 we active vascularization 14th regeneration avascular. 30th complete healing zone formation fibrosis slight vascularization, which partial deposition pigment zone. And 1 cat 15th perforation iris prolapse subsequent evisceration. Conclusion. This study shows is effective infectious keratitis, accompanied These results allow us offer as alternative methods treatment. method safe structures eye while observing recommended protocols, confirmed our work. However, given small number patients, further research required select optimal combination regimen.

Language: Английский