MSC-exosomes pretreated by Danshensu extracts pretreating to target the hsa-miR-27a-5p and STAT3-SHANK2 to enhanced antifibrotic therapy

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 4, 2025

Peritoneal fibrosis (PF) is a serious complication commonly associated with prolonged peritoneal dialysis. Mesenchymal stem cells (MSCs) and their exosomes (Exo) have shown significant therapeutic promise in treating fibrotic conditions. Danshensu (DSS), bioactive compound from the traditional Chinese herb Danshen reverses fibrosis. This study aims to investigate novel strategy enhance efficacy against PF by DSS preconditioning MSCs-derived (DSS-Exo). The vitro studies included effects of duration on MSCs, characterization DSS-Exo Exo, followed assessment RNA protein expression levels markers inflammatory cytokines after human mesothelial (HMrSV5) cells. In vivo experiments were conducted mouse model observe cell morphology, collagen deposition, localization, evaluate functions such as filtration rate, urea nitrogen clearance, thickness, leakage. Mechanistic insights gained through analysis STAT3/HIF-1α/VEGF signaling pathway, tissue dual-fluorescence localization,chromatin immunoprecipitation sequencing (ChIP-seq), dual-luciferase reporter (DLR) assays. Additionally, differential miRNAs between Exo was explored validation key miRNA. significantly upregulated E-cadherin, downregulated VEGFA, α-SMA, CTGF Fibronectin HMrSV5 compared untreated Exo. revealed that enhanced ability improve function,such rate nitrogen, glucose while reducing thickness leakage, reduce decrease degree Mechanistically, these inhibited pathway within tissues. Furthermore, ChIP-seq DLR demonstrated affected STAT3 directly binds SHANK2 promoter regions, forming hydrogen bonds 5 amino acids GLN-344, HIS-332 6 bases DG-258, DG-261. miRNA profiling identified increased hsa-miR-27a-5p_R-1 regulated STAT3-SHANK2 modulating EMT. highlighted innovative use enhancing MSC-derived exosome therapy for PF. identification hsa-miR-27a-5p_R-1-STAT3-SHANK2 axis may reveal new molecular mechanisms underlying fibrosis, further research needed fully elucidate its impact integration medicine into modern MSC represents promising frontier development treatments diseases.

Language: Английский

---

Exosomes as Therapeutic and Diagnostic Tools: Advances, Challenges, and Future Directions

Cell Biochemistry and Biophysics, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Language: Английский

---

Innovative Platforms in Regenerative Medicine: Bridging Research and Clinical Solutions

Jentashapir Journal of Cellular and Molecular Biology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 13, 2025

Context: Recent advances in induced pluripotent stem cells (iPSCs), CRISPR-Cas9 gene editing, nanotechnologies, and artificial intelligence have revolutionized regenerative medicine (RM) as a transformative field for tackling difficult medical problems. These breakthroughs promise specific treatments, proper restoration of tissue function, substantial improvements the quality life patients whose ailments cannot yet be cured. This review explores cutting-edge advancements RM platforms such cell therapy, 3D bioprinting, nanotechnology. The study also aims to shed light on challenges clinical translation policy implications, which are crucial fostering sustainable progressive discipline. Evidence Acquisition: manuscript draws research development application technologies. It synthesizes data cells, engineering, vitro organoid industry, (AI), nanotechnology that illustrate therapeutic potential. identify ethical, regulatory, practical hurdles translating from practice. Results: Breakthroughs those iPSC-derived organoids, nanostructured materials exhibit significant preclinical settings. Platforms organ-on-chip AI tools further enhance drug discovery treatment monitoring, while biomaterials scaffold-based approaches repair regeneration. Nevertheless, despite these advances, persist regarding scale-up, safety, ethical considerations. Conclusions: Innovations represent paradigm shift purely symptomatic treatments restorative therapies. Successful integration into practice will require multidisciplinary collaborative work, imposition rigorous safety protocols, enabling regulatory frameworks. Addressing would enable realize its true potential foundation 21st-century healthcare.

Language: Английский

---

Human umbilical cord mesenchymal stem cells promoted tumor cell growth associated with increased interleukin-18 in hepatocellular carcinoma

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: June 14, 2024

Language: Английский

---