Optimization of the production of Exopolysaccharide (EPS) from biofilm-forming bacterial consortium using different parameters

The Microbe, Journal Year: 2024, Volume and Issue: 4, P. 100117 - 100117

Published: July 4, 2024

Bacterial cells produce a variety of exopolysaccharide (EPS) throughout their life cycle. EPS plays crucial role in the formation biofilms and control environmental processes microbial communities. The bacterial community benefits from several ways, including nutrient compartmentation storage, stress resistance synergism. generation by consortium-associated bacteria, which involves microorganisms, is versatile efficient for use industrial processes, such as biotechnology pollution degradation. qualitative quantitative chemical characteristics consortium's are mostly determined type culture utilized, type, temperature, pH. main goal study was to find out how biosynthesis works consortium culture. To achieve maximum regeneration, pH, carbon, nitrogen sources were optimized. A three strains i.e. Klebsiella pneumonia strain, Pseudomonas aeruginosa strain Burkholderia cepacian that distinguished capacity developed part study. total production detected after 8 days incubation at pH 7.5 (15.5 g/L) temperature 35 °C (12.60 g/L). optimum identified 17.84 g/L 21.07 when ammonium sulfate glucose employed carbon supplements, respectively. protein concentration 3067 µg/mL, more than carbohydrate (263.10 µg/mL) DNA (7.60 content. protein/carbohydrate ratio present 11.65, much higher prior investigations. FTIR analysis confirmed functional groups carbohydrates, proteins, lipids DNA, viz., CO, COOH, NH, OH, CH OCH3. study's findings revealed enhancing molecules lowering biofilm-related infections improving efficacy antimicrobial treatments wastewater.

Language: Английский

Pharmaceutical industrial wastewater exhibiting the co-occurrence of biofilm-forming genes in the multidrug-resistant bacterial community poses a novel environmental threat

Aquatic Toxicology, Journal Year: 2024, Volume and Issue: 273, P. 107019 - 107019

Published: July 2, 2024

Language: Английский