Proteomic profiling of renal tissue of normo- and hypertensive rats with the renalase peptide RP220 as an affinity ligand

Biomeditsinskaya Khimiya, Journal Year: 2024, Volume and Issue: 70(3), P. 145 - 155

Published: Jan. 1, 2024

Renalase (RNLS) is a recently discovered protein that plays an important role in the regulation of blood pressure by acting inside and outside cells. Intracellular RNLS FAD-dependent oxidoreductase oxidizes isomeric forms β-NAD(P)H. Extracellular renalase lacking its N-terminal peptide cofactor FAD exerts various protective effects via non-catalytic mechanisms. Certain experimental evidence exists literature RP220 (a 20-mer corresponding to amino acid sequence 220-239) reproduces number this protein, on receptor proteins plasma membrane. The possibility interaction with intracellular has not been studied. Taking into consideration known as possible antihypertensive factor, aim study was perform proteomic profiling kidneys normotensive hypertensive rats using affinity ligand. Proteomic (semi-quantitative) identification revealed changes relative content about 200 individual bound sorbent compared animals. Increased binding SHR renal over control found for involved development cardiovascular pathology. Decreased kidney from animals noted components ubiquitin-proteasome system, ribosomes, cytoskeleton.

Language: Английский

The Role of Renal Medullary Bilirubin and Biliverdin Reductase in Angiotensin II-Dependent Hypertension

American Journal of Hypertension, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Increased circulating bilirubin attenuates angiotensin (Ang) II-induced hypertension and improves renal hemodynamics. However, the intrarenal mechanisms that mediate these effects are not known. The goal of present study was to test hypothesis generation in medulla plays a protective role against Ang hypertension.

Language: Английский