Cone-rod homeobox transcriptionally activates TCF7 to promote the proliferation of retinal pigment epithelial and retinoblastoma cells in vitro DOI
Na Zhao, Yingying Li, J. Xu

et al.

International Journal of Ophthalmology, Journal Year: 2024, Volume and Issue: 17(11), P. 1995 - 2006

Published: Oct. 23, 2024

AIM: To investigate the proliferation regulatory effect of cone-rod homeobox (CRX) in retinal pigment epithelium (RPE) and retinoblastoma (RB) cells to explore potential application side (oncogenic potential) CRX-based gene therapy RPE-based retinopathies. METHODS: Adult human epithelial (ARPE)-19 (RPE)-1 Y79 RB cell were used study. Genetic manipulation was performed by lentivirus-based technology. The determined a CellTiter-Glo Reagent. mRNA protein levels quantitative real-time polymerase chain reaction (qPCR) Western blot assay. transcriptional activity promoter luciferase reporter bindings between CRX transcription factor 7 (TCF7) as well TCF7 promoters target genes examined chromatin immunoprecipitation (ChIP) modified nuclear run-on RESULTS: overexpression knockdown significantly increased (n=3, P<0.05 all cells) decreased P<0.01 RPE cells. deceased Wnt signaling [including MYC proto-oncogene (MYC), JUN, FOS like 1 (FOSL1), CCND1, cyclin D2 (CCND2), D3 (CCND3), cellular communication network 4 (CCN4), peroxisome proliferator activated receptor delta (PPARD), matrix metallopeptidase (MMP7)] driven (TCF7). depletion abolished stimulatory on level immunoprecipitated antibody. CONCLUSION: transcriptionally activates promote vitro. is for regenerative medicine. risk this strategy, tumorigenic potential, should be considered.

Language: Английский

Polyaminoglycoside nanosystem expressing antimicrobial peptides for multistage chronic wound management DOI

Rui Ju,

Yang Li, Dandan Sui

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113657 - 113657

Published: March 1, 2025

Language: Английский

Citations

0
Cone-rod homeobox transcriptionally activates TCF7 to promote the proliferation of retinal pigment epithelial and retinoblastoma cells in vitro DOI
Na Zhao, Yingying Li, J. Xu

et al.

International Journal of Ophthalmology, Journal Year: 2024, Volume and Issue: 17(11), P. 1995 - 2006

Published: Oct. 23, 2024

AIM: To investigate the proliferation regulatory effect of cone-rod homeobox (CRX) in retinal pigment epithelium (RPE) and retinoblastoma (RB) cells to explore potential application side (oncogenic potential) CRX-based gene therapy RPE-based retinopathies. METHODS: Adult human epithelial (ARPE)-19 (RPE)-1 Y79 RB cell were used study. Genetic manipulation was performed by lentivirus-based technology. The determined a CellTiter-Glo Reagent. mRNA protein levels quantitative real-time polymerase chain reaction (qPCR) Western blot assay. transcriptional activity promoter luciferase reporter bindings between CRX transcription factor 7 (TCF7) as well TCF7 promoters target genes examined chromatin immunoprecipitation (ChIP) modified nuclear run-on RESULTS: overexpression knockdown significantly increased (n=3, P<0.05 all cells) decreased P<0.01 RPE cells. deceased Wnt signaling [including MYC proto-oncogene (MYC), JUN, FOS like 1 (FOSL1), CCND1, cyclin D2 (CCND2), D3 (CCND3), cellular communication network 4 (CCN4), peroxisome proliferator activated receptor delta (PPARD), matrix metallopeptidase (MMP7)] driven (TCF7). depletion abolished stimulatory on level immunoprecipitated antibody. CONCLUSION: transcriptionally activates promote vitro. is for regenerative medicine. risk this strategy, tumorigenic potential, should be considered.

Language: Английский

Citations

0