Long intergenic noncoding RNA-p21 inhibits apoptosis by decreasing PUMA expression in non-small cell lung cancer DOI Creative Commons

Tao Yang,

Wenjun Zhang, Li Wang

et al.

Journal of International Medical Research, Journal Year: 2018, Volume and Issue: 47(1), P. 481 - 493

Published: Dec. 16, 2018

Objective Long noncoding RNAs (lncRNAs) are important mediators in tumor progression. intergenic RNA-p21 (lincRNA-p21) participates multiple biological processes. This study explored the role of lincRNA-p21 human non-small cell lung cancer (NSCLC) progression and potential regulatory mechanisms. Methods LincRNA-p21 expression NSCLC tissues lines (A549, H1299, H1650, NCI-H2087) was determined by quantitative real-time PCR. overexpressing sh-lincRNA-p21 lentiviral were respectively transfected into H1299 A549 cells. Flow cytometry used to measure apoptosis. Microarray analysis RNA pull-down assay predict target genes lincRNA-p21. Finally, PUMA siRNA cells, extent apoptosis measured. The protein levels relative confirmed western blot analysis. Results significantly upregulated upregulation considerably inhibited while downregulation showed opposite effect. a direct gene negatively correlated with specimens. anti-apoptotic effect can be effectively attenuated PUMA. Conclusion is aberrantly inhibits decreasing expression.

Language: Английский

LncRNA-LINC00460 facilitates nasopharyngeal carcinoma tumorigenesis through sponging miR-149-5p to up-regulate IL6 DOI

Yonggang Kong,

Min Cui, Shiming Chen

et al.

Gene, Journal Year: 2017, Volume and Issue: 639, P. 77 - 84

Published: Oct. 11, 2017

Language: Английский

Citations

111
Long noncoding RNA DLX6-AS1 promotes renal cell carcinoma progression via miR-26a/PTEN axis DOI Open Access
Xing Zeng, Zhiquan Hu,

Xinwen Ke

et al.

Cell Cycle, Journal Year: 2017, Volume and Issue: 16(22), P. 2212 - 2219

Published: Sept. 7, 2017

Recently, long non-coding RNAs (lncRNAs) have emerged as new gene regulators and prognostic markers in several types of cancer, including renal cell carcinoma (RCC). In this study, we identified an upregulated lncRNA, DLX6-AS1, RCC tumor tissues compared with normal kidney tissues. Our data suggested that DLX6-AS1 promoted growth tumorigenesis via targeting miR-26a. addition, observed PTEN overexpression restored the cancer also rescued tumorigenesis. summary, conclude promotes development regulation miR-26a/PTEN axis.

Language: Английский

Citations

64
Dysregulation of pseudogene/lncRNA-hsa-miR-363-3p-SPOCK2 pathway fuels stage progression of ovarian cancer DOI Creative Commons
Weiyang Lou,

Bisha Ding,

Guansheng Zhong

et al.

Aging, Journal Year: 2019, Volume and Issue: 11(23), P. 11416 - 11439

Published: Dec. 3, 2019

Ovarian cancer is one of the most common and lethal types in women. The molecular mechanism ovarian progression still unclear.Here, we first reported that expression levels three genes, GJB2, S100A2 SPOCK2, were significantly higher advanced stage than early cancer, upregulation them indicated poor prognosis patients with cancer. Subsequently, 8, 6 20 miRNAs predicted to target respectively. Among these miRNA-mRNA pairs, hsa-miR-363-3p-SPOCK2 axis was potential suppressing Mechanistically, found involved regulation actin cytoskeleton. Moreover, pseudogenes 8 lncRNAs identified potentially inhibit cancer.Collectively, elucidate a regulatory role pseudogene/lncRNA-hsa-miR-363-3p-SPOCK2 pathway which may provide effective therapeutic approaches promising prognostic biomarkers for cancer.Differentially expressed genes (DEGs) screened using GSE12470, after DEGs validated GEPIA. Kaplan-Meier analysis employed assess values. Potential by seven prediction databases, upstream hsa-miR-363-3p forecasted through miRNet or starBase. UALCAN starBase used obtain co-expressed SPOCK. Enrichment performed Enrichr.

Language: Английский

Citations

62
Long intergenic non-coding RNA 668 regulates VEGFA signaling through inhibition of miR-297 in oral squamous cell carcinoma DOI

Chen-Zheng Zhang

Biochemical and Biophysical Research Communications, Journal Year: 2017, Volume and Issue: 489(4), P. 404 - 412

Published: May 30, 2017

Language: Английский

Citations

58
Long Non-coding RNA LINC00339 Stimulates Glioma Vasculogenic Mimicry Formation by Regulating the miR-539-5p/TWIST1/MMPs Axis DOI Creative Commons
Junqing Guo,

Heng Cai,

Xiaobai Liu

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2017, Volume and Issue: 10, P. 170 - 186

Published: Nov. 26, 2017

Glioma is recognized as a highly angiogenic malignant brain tumor. Vasculogenic mimicry (VM) greatly restricts the therapeutic effect of anti-angiogenic tumor therapy for glioma patients. However, molecular mechanisms VM formation in remain unclear. Here, we demonstrated that LINC00339 was upregulated tissue well cell lines. The expression tissues positively correlated with formation. Knockdown inhibited proliferation, migration, invasion, and tube formation, meanwhile downregulating VM-related MMP-2 MMP-14. Furthermore, knockdown significantly increased miR-539-5p. Both bioinformatics luciferase reporter assay revealed regulated above effects via binding to Besides, overexpression miR-539-5p resulted decreased TWIST1, transcription factor known play an oncogenic role identified direct target TWIST1 promoter activities vivo study showed nude mice carrying tumors exhibited smallest volume through inhibiting In conclusion, may be used novel glioma.

Language: Английский

Citations

58
Long non-coding RNA PCAT7 regulates ELF2 signaling through inhibition of miR-134-5p in nasopharyngeal carcinoma DOI
Yejun Liu, Zezhang Tao,

Jining Qu

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2017, Volume and Issue: 491(2), P. 374 - 381

Published: July 17, 2017

Language: Английский

Citations

55
Long non-coding RNA FOXD2-AS1 aggravates nasopharyngeal carcinoma carcinogenesis by modulating miR-363-5p/S100A1 pathway DOI
Gang Chen, Wenjie Sun,

Xinying Hua

et al.

Gene, Journal Year: 2017, Volume and Issue: 645, P. 76 - 84

Published: Dec. 14, 2017

Language: Английский

Citations

55
NKILA represses nasopharyngeal carcinoma carcinogenesis and metastasis by NF-κB pathway inhibition DOI Creative Commons
Wei Zhang, Qiannan Guo,

Guoying Liu

et al.

PLoS Genetics, Journal Year: 2019, Volume and Issue: 15(8), P. e1008325 - e1008325

Published: Aug. 20, 2019

The role of long non-coding RNA (lncRNA) in the progression Nasopharyngeal carcinoma (NPC) has not been fully elucidated. study was designed to explore functional NKILA, a newly identified lncRNA, NPC. We performed lncRNA expression profile microarray using four NPC and paired para-cancerous tissues. NKILA as potential by this profile. used 107 paraffin-embedded tissues with different TNM stages detect analyzed survival data Log-rank test Cox regression. its underlying mechanisms were evaluated series experiments vitro vivo silencing or expressing NKILA. Compared control tissues, be decreased Low correlated unfavorable clinicopathological features predicted poor outcome patients. After adjusting for confounders, low confirmed an independent prognostic factor outcomes. Furthermore, we found that overexpression high-metastatic-potential cells repressed motile behavior impaired metastatic capacity vivo. In contrast, RNAi-mediated depletion increased invasive motility lower potential. Further demonstrated regulated metastasis through NF-κB pathway. Taken together, plays vital roles pathogenesis unique histological characteristics indicate local inflammation carcinogenesis nasopharyngeal carcinoma.

Language: Английский

Citations

54
DANCR contributed to hepatocellular carcinoma malignancy via sponging miR‐216a‐5p and modulating KLF12 DOI
Jianchu Wang, Jian Pu,

Ying Zhang

et al.

Journal of Cellular Physiology, Journal Year: 2018, Volume and Issue: 234(6), P. 9408 - 9416

Published: Nov. 14, 2018

Long noncoding RNA (lncRNA) differentiation antagonizing nonprotein coding (DANCR) has been identified as an oncogene in several cancers. However, the biological function and role of DANCR hepatocellular carcinoma (HCC) remain unclear. Our current study aimed to investigate detailed mechanism HCC. We found that was significantly upregulated HCC cell lines comparison LO2 cells. Then, we observed knockdown could greatly inhibit Huh7 HepG2 proliferation. In addition, apoptosis increased by silence meanwhile, cycle progression blocked G1 phase. Apart from these, downregulation repressed migration invasion ability obviously. As predicted bioinformatics analysis, microRNA-216a-5p (miR-216a-5p) serve a direct target DANCR. MiR-216a-5p reported be involved many Here, correlation between miR-216a-5p confirmed using dual-luciferase reporter assay radioimmunoprecipitation assay. Subsequently, Kruppel-like factor 12 (KLF12) exerts important different tumor types. KLF12 can downstream miR-216a-5p. Finally, vivo experiments were used data proved also strongly suppressed growth via targeting KLF12. conclusion, our indicated might provide new perspective for treatment.

Language: Английский

Citations

51
HOXD-AS1 functions as an oncogenic ceRNA to promote NSCLC cell progression by sequestering miR-147a DOI Open Access
Qinghua Wang, Shujun Jiang, Anying Song

et al.

OncoTargets and Therapy, Journal Year: 2017, Volume and Issue: Volume 10, P. 4753 - 4763

Published: Sept. 1, 2017

Abstract: Non-small cell lung cancer (NSCLC) is one of the most common malignancies worldwide, and it occurs at a higher frequency in males. HOXD-AS1, an important cancer-associated long noncoding RNA (lncRNA), contributes to development progression several cancers. However, exact roles HOXD-AS1 NSCLC are still unknown. Here, we investigated underlying mechanisms human tissues. We found that lncRNA was specifically upregulated ( P <0.001) tissues promoted growth by targeting miR-147a. Moreover, expression positively correlated with clinical pathologic characteristics (tumor size, =0.006; tumor stage, =0.044; recurrence, =0.031) survival rate =0.003). knockdown reduced proliferation apoptosis cells. The dual-luciferase reporter assay showed could negatively regulate miR-147a inhibition abrogated effect on Furthermore, regulated pRB (a suppressor protein) Taken together, our data indicated might be oncogenic promotes therapeutic target NSCLC. Keywords: non-small cancer, proliferation,

Language: Английский

Citations

50