Dysregulation of Long Non-coding RNAs and mRNAs in Plasma of Clear Cell Renal Cell Carcinoma Patients Using Microarray and Bioinformatic Analysis DOI
Bing Zhang, Wei Chu,

Feifei Wen

et al.

Frontiers in Oncology, Journal Year: 2020, Volume and Issue: 10

Published: Nov. 27, 2020

Objective: The roles of long non-coding RNAs (lncRNAs) in the diagnosis clear cell renal carcinoma (ccRCC) are still not well-defined. We aimed to identify differentially expressed lncRNAs and mRNAs plasma ccRCC patients health controls systematically. Methods: Expression profile healthy was analyzed based on microarray assay. Gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway-based approaches were used investigate biological function signaling pathways mediated by mRNAs. SOCS2-AS1 selected for validation using Real-Time PCR. further compared with E-MTAB-1830 datasets Venn NetworkAnalyst website. GEPIA ULCAN websites utilized evaluation expression level mRNA their association overall survival (OS). Results: A total 3,664 identified patients, including 1,511 up-regulated 2,153 down-regulated (fold change ≥2 P < 0.05), respectively. There 2,268 mRNAs, 932 1,336 respectively 0.05). Pathway analysis deregulated mainly involved melanogenesis Hippo pathway ( In line lncRNA findings, tissues, as well lines. Compared gene profiles, we 18 87 differently both neoplastic tissues ccRCC. 10 EPB41L4B, CCND1, GGT1, CGNL1, CYSLTR1, PLAUR, UGT3A1, PROM2, MUC12 , PCK1 ) correlated (OS) rate websites. Conclusions: firstly reported systemically. Several identified, which might serve diagnostic or prognostic markers. these should be validated. Our study may contribute future treatment provide novel insights into cancer biology.

Language: Английский

Downregulation of lncRNA APCDD1L-AS1 due to DNA hypermethylation and loss of VHL protein expression promotes the progression of clear cell renal cell carcinoma DOI Creative Commons
Wuping Yang, Jingcheng Zhou, Zedan Zhang

et al.

International Journal of Biological Sciences, Journal Year: 2022, Volume and Issue: 18(6), P. 2583 - 2596

Published: Jan. 1, 2022

Background:The current studies only indicated that long non-coding RNA (lncRNA) APCDD1L-AS1, as a novel lncRNA, may play role in oral squamous cell carcinoma and lung cancer.However, its potential clear renal (ccRCC) possible mechanism of action remain vague.Methods: TCGA-KIRC GEO data qRT-PCR pyrosequencing results clinical specimens were used to identify the expression level DNA methylation status APCDD1L-AS1.The effects APCDD1L-AS1 overexpression on ccRCC growth metastasis determined by function experiments.Western blot Tandem mass tags (TMT) utilized explore relationship between VHL downstream underlying mechanisms.Results: The was downregulated ccRCC.Decreased related higher tumor stage histological grade shorter RFS (Relapse-free survival).Besides, restrained cells vitro vivo.Moreover, reduced could be caused hypermethylation loss von Hippel Lindau (VHL) protein expression.Furthermore, dysregulation histones one important mechanisms suppress progression ccRCC.Conclusion: able inhibit ccRCC, decreased expression.Therefore, serve new therapeutic target treatment ccRCC.

Language: Английский

Citations

12
Non-coding RNAs in renal cell carcinoma: Implications for drug resistance DOI Open Access
Yiqi Liu, Hu Zhang,

Yuan Fang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 164, P. 115001 - 115001

Published: June 12, 2023

Renal cell carcinoma (RCC) represents a malignant tumor of the urinary system. Individuals with early-stage RCC could be cured by surgical treatment, but considerable number cases advanced progress to drug resistance. Recently, numerous reports have demonstrated that variety non-coding RNAs (ncRNAs) contribute occurrence and development. ncRNAs can act as oncogenic or suppressor genes regulate proliferation, migration, resistance other processes in cells through signaling pathways. Considering lack treatment options for after resistance, may good choice biomarkers targets overcome In this review, we discussed effects on great potential biomarker new therapeutic method RCC.

Language: Английский

Citations

7
Long noncoding RNA SNHG6 promotes carcinogenesis by enhancing YBX1‐mediated translation of HIF1α in clear cell renal cell carcinoma DOI
Ping Zhao, Yu Deng, Yong Wu

et al.

The FASEB Journal, Journal Year: 2020, Volume and Issue: 35(2)

Published: Nov. 4, 2020

Recent studies have showed that Small nucleolar RNA host genes (SNHGs) acted as a subset of long noncoding RNAs (lncRNAs) critical roles in human cancer carcinogenesis. However, the biological functions SNHGs clear cell renal carcinoma (ccRCC) not been fully investigated. In this study, we screened an oncogenic lncRNA termed SNHG6 using RNA-Seq data ccRCC from The Cancer Genome Atlas (TCGA). Quantitative real-time PCR was then used to demonstrate expression tissues. overexpression is highly associated with malignant features patients and prognostic indicator. significantly promotes proliferation metastasis vitro vivo. Mechanistic investigations identified exerts effects by interacting YBX1, then, enhancing HIF1α translation. Taken together, progression binding YBX1 may serve novel molecular target for therapy.

Language: Английский

Citations

17
From Micro to Long: Non-Coding RNAs in Tamoxifen Resistance of Breast Cancer Cells DOI Open Access

Jéssica Fernanda Barazetti,

Tayana Shultz Jucoski,

Tamyres Mingorance Carvalho

et al.

Cancers, Journal Year: 2021, Volume and Issue: 13(15), P. 3688 - 3688

Published: July 22, 2021

Breast cancer is the most commonly diagnosed and leading cause of mortality among women. Two thirds patients are classified as hormone receptor positive, based on expression estrogen alpha (ERα), main driver breast cell proliferation, and/or progesterone receptor, which regulated by ERα. Despite presenting best prognosis, these tumors can recur when acquire resistance to treatment aromatase inhibitors or antiestrogen such tamoxifen (Tam). The mechanisms that involved in Tam complex involve multiple signaling pathways. Recently, roles for microRNAs lncRNAs controlling ER action have been described, but underlying still little explored. In this review, we will discuss current state knowledge positive cancer. future, be used identify at a greater risk relapse due patterns ncRNAs impact response Tam, order guide their more efficiently possibly design therapeutic strategies bypass resistance.

Language: Английский

Citations

15
Dysregulation of Long Non-coding RNAs and mRNAs in Plasma of Clear Cell Renal Cell Carcinoma Patients Using Microarray and Bioinformatic Analysis DOI
Bing Zhang, Wei Chu,

Feifei Wen

et al.

Frontiers in Oncology, Journal Year: 2020, Volume and Issue: 10

Published: Nov. 27, 2020

Objective: The roles of long non-coding RNAs (lncRNAs) in the diagnosis clear cell renal carcinoma (ccRCC) are still not well-defined. We aimed to identify differentially expressed lncRNAs and mRNAs plasma ccRCC patients health controls systematically. Methods: Expression profile healthy was analyzed based on microarray assay. Gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway-based approaches were used investigate biological function signaling pathways mediated by mRNAs. SOCS2-AS1 selected for validation using Real-Time PCR. further compared with E-MTAB-1830 datasets Venn NetworkAnalyst website. GEPIA ULCAN websites utilized evaluation expression level mRNA their association overall survival (OS). Results: A total 3,664 identified patients, including 1,511 up-regulated 2,153 down-regulated (fold change ≥2 P < 0.05), respectively. There 2,268 mRNAs, 932 1,336 respectively 0.05). Pathway analysis deregulated mainly involved melanogenesis Hippo pathway ( In line lncRNA findings, tissues, as well lines. Compared gene profiles, we 18 87 differently both neoplastic tissues ccRCC. 10 EPB41L4B, CCND1, GGT1, CGNL1, CYSLTR1, PLAUR, UGT3A1, PROM2, MUC12 , PCK1 ) correlated (OS) rate websites. Conclusions: firstly reported systemically. Several identified, which might serve diagnostic or prognostic markers. these should be validated. Our study may contribute future treatment provide novel insights into cancer biology.

Language: Английский

Citations

16