Rapid and precise genome engineering in a naturally short-lived vertebrate
eLife,
Journal Year:
2023,
Volume and Issue:
12
Published: May 9, 2023
The
African
turquoise
killifish
is
a
powerful
vertebrate
system
to
study
complex
phenotypes
at
scale,
including
aging
and
age-related
disease.
Here,
we
develop
rapid
precise
CRISPR/Cas9-mediated
knock-in
approach
in
the
killifish.
We
show
its
efficient
application
precisely
insert
fluorescent
reporters
of
different
sizes
various
genomic
loci
order
drive
cell-type-
tissue-specific
expression.
This
method
should
allow
establishment
humanized
disease
models
development
cell-type-specific
molecular
probes
for
studying
biology.
Language: Английский
Early-life growth and cellular heterogeneity in the short-lived African turquoise killifish telencephalon
Biology Open,
Journal Year:
2025,
Volume and Issue:
14(4)
Published: April 15, 2025
ABSTRACT
The
African
turquoise
killifish
(Nothobranchius
furzeri)
is
becoming
a
favorable
model
for
neurobiological
research.
combination
of
short
lifespan
and
declining
neuroregenerative
capacity
upon
aging
makes
it
ideally
suited
research
on
brain
regeneration.
A
remarkable
cellular
diversity
up
the
young-adult
telencephalon,
characterized
by
highly
proliferative
non-glial
progenitors
spatially
distinct
radial
glia
subtypes.
In
contrast
to
relatively
slow
embryonic
development,
hatching
followed
period
accelerated
growth,
in
which
experiences
rapid
expansion
maturation.
this
study,
we
quantified
growth
progression
maturation
telencephalon
during
early
post-embryonic
development.
We
discovered
that,
similar
zebrafish,
neuro-epithelial
cells
abut
neurogenic
niches
from
life
onwards.
Spatial
data
revealed
qualitative
quantitative
differences
along
anterior-posterior
axis
between
pallial
subpallial
regions
terms
pace.
confirmed
generation
GABAergic
neurons
niche
glutamatergic
two
niches.
Our
further
showed
more
widespread
appearance
inhibitory
at
compared
zebrafish.
Language: Английский
A review on neurodegeneration in the fast-ageing killifish, the first animal model to study the natural occurrence of neuronal cell loss
Ageing Research Reviews,
Journal Year:
2023,
Volume and Issue:
91, P. 102065 - 102065
Published: Sept. 3, 2023
Language: Английский
The African Turquoise Killifish: A Scalable Vertebrate Model for Aging and Other Complex Phenotypes
Cold Spring Harbor Protocols,
Journal Year:
2023,
Volume and Issue:
2024(3), P. pdb.over107737 - pdb.over107737
Published: April 26, 2023
The
African
turquoise
killifish
Language: Английский