The effect of vine tea (Ampelopsis grossedentata) extract on fatigue alleviation via improving muscle mass

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 325, P. 117810 - 117810

Published: Jan. 22, 2024

Language: Английский

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Hong-Bai-Lan-Shen Extract Alleviates the CoCl2-Induced Apoptosis in H9C2 Cells by Regulating the AMPK Pathway

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(3), P. 267 - 267

Published: March 13, 2025

This study aims to explore the protective effects of Hong-bai-lan-shen (HBLS) extract, a traditional Chinese medicine compound, on myocardial injury based metabolomics. H9C2 cells were cultured with HBLS extract for 12 h, and then in CoCl2-containing medium, model simulating ischemic-hypoxic damage cells, an additional h. The cell viability, cytotoxicity, intracellular metabolite reactive oxygen species (ROS), mitochondrial membrane potential, apoptosis, adenosine monophosphate-activated protein kinase (AMPK) signal pathway determined. results showed that significantly increased stabilized morphology, reduced lactate dehydrogenase (LDH) release ROS production, blocked cysteine-aspartic acid protease 3 (caspase-3) bcl-2-associated X (Bax) expression decreased apoptotic numbers. Meanwhile, potential B-cell lymphoma-2 (Bcl-2). Additionally, upregulated AMPK, PI3K, B (AKT) (p < 0.05, p 0.01). These findings suggest has effect by regulating AMPK may be promising therapeutic candidate ischemic heart disease.

Language: Английский

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Polyphenols and post-exercise muscle damage: a comprehensive review of literature

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: April 9, 2025

Abstract Recent research highlights the significant role of polyphenols in alleviating post-exercise muscle damage, thus positioning them as a valuable nutritional strategy for athletes and fitness enthusiasts. Polyphenols, naturally occurring bioactive compounds abundant fruits, vegetables, tea, wine, other plant-based foods, are recognized their potent antioxidant anti-inflammatory properties. This dual mechanism is critical combating oxidative stress inflammation—two factors that intensify during vigorous physical activity contribute to soreness damage. Among various polyphenols, like quercetin have particularly emerged effective agents promoting recovery enhancing exercise performance. These protective effects facilitated through several mechanisms, including modulation inflammatory pathways, acceleration repair processes, enhancement mitochondrial function, all which bolster overall health. As ongoing studies yield deeper insights, potential enhance athletic performance health will become increasingly substantiated, leading towards strategic incorporation into nutrition protocols. Therefore, we reviewed relevant order show how efficient can be reducing fatigue damage what exact mechanisms.

Language: Английский

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Evaluation of antimicrobial photodynamic activity of CuminUP60®- A commercially-available high-soluble curcumin

LWT, Journal Year: 2024, Volume and Issue: 205, P. 116490 - 116490

Published: July 14, 2024

Curcumin, a polyphenol extracted from Curcuma longa L. with variety of biological and pharmacological activities, has been identified as promising photosensitizer for food sterilization. However, its low aqueous solubility bioavailability greatly restrict the practical application curcumin. In last decades, number approaches have proposed to address this challenge, several having already attained notable commercial viability. CuminUP60®, patented curcumin complex consisting extract Poloxam407, gathered attention in realms enhanced solubility, therapeutic potential. antimicrobial photodynamic activity novel not investigated. current study, we found that CuminUP60® showed maximum absorption intensity at 434 nm capability produce Reactive Oxygen Species (ROS) upon blue light excitation. Furthermore, desirable bactericidal against range typical foodborne bacteria including Staphylococcus aureus, Listeria monocytogenes Vibrio parahaemolyticus, Shewanella putrefaciens, Pseudomonas fluorescens (MIC = 83.33 μM) Escherichia coli 166.66 (5.3 mW/cm2) illumination 30 min. Taken together, results demonstrated possess antibacterial activity, which could provide crucial support development industrially applicable sterilization systems within industry.

Language: Английский

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Preliminary investigation of anti-fatigue effects and potential mechanisms of meiju oral liquid in mouse and zebrafish models

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0316761 - e0316761

Published: March 6, 2025

Meiju Oral Liquid (MOL), a representative medicinal formula in China, stems from the traditional use of specific Chinese herbs known for their anti-fatigue properties, including rose, jujube, chicory, and wolfberry. While these individual have been recognized benefits, formulation MOL itself has not extensively studied. This study was designed to evaluate potential effects MOL, prepared natural herbs, explore its underlying mechanisms. In this research, both mouse zebrafish models were utilized investigate MOL. Chemical characterization identification bioactive compounds serum conducted using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). The results demonstrated that significantly prolonged weight-bearing swimming time mice, increased hepatic muscle glycogen content, reduced levels blood urea nitrogen, lactate, inflammatory markers (IL-1β, IL-6, TNF-α, NO). Furthermore, down-regulated expression NOX4 TNF-α proteins while up-regulating p-PI3K p-AKT liver tissues fatigued mice. models, exhibited protective against sodium sulfite-induced lethality, enhanced high-speed motion trajectories, movement distances normal zebrafish. Additionally, downregulated IL-1β, TNF-β mRNA PI3K AKT1 These findings suggested may be mediated through activation PI3K/AKT signaling pathway as well inhibition expression. addition, total ninety-four chemical components identified with twenty-three migration detected serum. are likely primary active agents, contributing reduction metabolite accumulation, enhancement synthesis, suppression responses. Taken together, our underscore warranting further investigation into therapeutic applications roles compounds.

Language: Английский

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Curcumin's Dose-Dependent Attenuation of Gastric Cancer Cell Progression Via the PI3K Pathway Blockade

Dose-Response, Journal Year: 2023, Volume and Issue: 21(4)

Published: Oct. 1, 2023

Background: Gastric cancer stands as a primary cause of cancer-related deaths worldwide, making the discovery new therapeutic agents essential for enhancing treatment outcomes. Curcumin, polyphenolic compound found in turmeric (Curcuma longa), has demonstrated potential multiple types due to its anti-cancer characteristics. This research aimed examine impact curcumin on gastric cell growth, migration, and invasion, well influence phosphoinositide 3-kinase (PI3K) signaling cascade. Methods: lines were exposed varying concentrations, followed by assessments viability, invasion. Furthermore, gene protein expression levels associated with PI3K cascade evaluated using reverse transcription-polymerase chain reaction (RT-PCR) Western blot analysis. Results: The findings revealed dose-dependent decrease invasion cells treated curcumin. Additionally, administration led downregulation key genes proteins within process, such PI3K, Akt, mTOR. Conclusion: These propose that may exercise effects cells, partly suppressing pathway. study's outcomes support curcumin's agent encourage further exploration underlying molecular mechanisms vivo effectiveness.

Language: Английский

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Signaling pathways regulated by natural active ingredients in the fight against exercise fatigue-a review

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 14, 2023

Exercise fatigue is a normal protective mechanism of the body. However, long-term hinders metabolism and exercise capacity. The generation recovery from involves alterations in multiple signaling pathways, mainly AMPK, PI3K/Akt, Nrf2/ARE, NF-κB, PINK1/Parkin, BDNF/TrkB, as well MAPK pathways that mediate energy supply, reduction metabolites, oxidative stress homeostasis, muscle fiber type switching, central effects. In recent studies, rich variety natural active ingredients have been identified traditional Chinese medicines plant extracts with anti-fatigue effects, opening up field research new drugs. this review we give an overview associated activity food against fatigue. Such comprehensive necessary to understand potential these materials preventive measures treatments We expect findings highlighted discussed here will help guide development health products provide theoretical scientific basis for future on

Language: Английский

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Bioactive Compounds in Citrus reticulata Peel Are Potential Candidates for Alleviating Physical Fatigue through a Triad Approach of Network Pharmacology, Molecular Docking, and Molecular Dynamics Modeling

Nutrients, Journal Year: 2024, Volume and Issue: 16(12), P. 1934 - 1934

Published: June 18, 2024

Physical fatigue (peripheral fatigue), which affects a considerable portion of the world population, is decline in ability muscle fibers to contract effectively due alterations regulatory processes action potentials. However, it lacks an efficacious therapeutic intervention. The present study explored bioactive compounds and mechanism Citrus reticulata peel (CR-P) treating physical by utilizing network pharmacology (NP), molecular docking, simulation-based dynamics (MD). ingredients CR-P prospective targets were obtained from various databases. A PPI was generated STRING database, while key overlapping analyzed for enrichment adopting KEGG GO. binding affinities hub determined docking. results further validated MD simulation. Five screened, 56 identified fatigue, whereas with greater degree AKT1, TP53, STAT3, MTOR, KRAS, HRAS, JAK2, IL6, EGFR, ESR1. findings analysis indicated significant three signaling pathways, namely PI3K-AKT, MAPK, JAK-STAT. docking simulation revealed that exhibit stronger affinity interacting targets. work suggests CR-P, specifically Hesperetin Sitosterol, may ameliorate via PI3K-AKT pathway targeting MTOR proteins.

Language: Английский

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Ren-Shen-Bu-Qi decoction alleviates exercise fatigue through activating PI3K/AKT/Nrf2 pathway in mice

Chinese Medicine, Journal Year: 2024, Volume and Issue: 19(1)

Published: Nov. 5, 2024

Abstract Background Fatigue is a prevalent issue that can lead individuals to sub-health condition, impacting their work efficiency and quality of life. There are limited effective treatment options available for fatigue. Ren-Shen-Bu-Qi decoction (RSBQD) proprietary herbal remedy designed address However, the specific pharmacological mechanisms basis RSBQD not yet fully understood. Purpose This study aimed investigate effects in mouse model exercise Materials methods UPLC-Q-Orbitrap HRMS was used analyze chemical composition RSBQD. The molecular mechanism on fatigue were predicted using network pharmacology analysis. Subsequently, an established analysis potential verified by hematoxylin–eosin (HE) staining, real-time fluorescence quantitative PCR (RT-qPCR), Western blot (WB) docking. Results results showed 88 main components identified, which have mainly belonged flavonoids carboxylic acid compounds. indicated ameliorate through PI3K/AKT signaling pathway. Notably, prolonged swimming time diminished body weight loss mice ( P < 0.05). Meanwhile, significantly alleviated injury liver kidney induced exhaustive exercise, decreasing serum alanine aminotransferase (ALT), aspartate (AST), urea BUN levels In addition, found could relieve content creatine kinase (CK), lactate dehydrogenase (LDH), lactic (LA), but increasing blood glucose (GLU) glycogen (HG) also increased hepatic superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) decreased malondialdehyde (MDA) levels. Moreover, able upregulate protein level activated Nrf2 pathways. Conclusions mitigates reversing metabolic changes reducing oxidative damage PI3K/AKT/Nrf2 offers support utilization treatment. Graphical

Language: Английский

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Curcumin and its nano‐formulations combined with exercise: From molecular mechanisms to clinic

Cell Biochemistry and Function, Journal Year: 2024, Volume and Issue: 42(4)

Published: May 29, 2024

Curcumin is a strong substance derived from turmeric, popular spice, renowned for its antioxidant and anti-inflammatory abilities. The study delved deeply into thorough examination of various sources to evaluate the impact both regular curcumin nano-formulated on elements that physical performance, including muscular strain, discomfort, swelling, oxidative tension. While engaging in exercise, body experiences rise reactive oxygen species inflammation. As result, it important ensure proper balance between internal external antioxidants maintain stability skeletal muscle. Without this balance, there risk muscle soreness, damage, ultimately, decline exercise performance. possesses ability enhance performance reduce symptoms fatigue injury by virtue antioxidative properties. Including supplements appears have advantageous effects aspects such as enhancing assisting with recovery, lessening damage lowering levels inflammation stress. However, assessment necessary precisely gauge healing advantages reducing recovery time.

Language: Английский

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