Characterizing macrophage diversity in colorectal malignancies through single-cell genomics

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 21, 2025

Colorectal cancer (CRC) is one of the most common malignant tumors digestive tract, with increasing incidence and mortality rates, posing a significant burden on human health. Its progression relies various mechanisms, among which tumor microenvironment tumor-associated macrophages (TAMs) have garnered attention. Macrophage infiltration in solid associated poor prognosis linked to chemotherapy resistance many cancers. These biological behaviors depend heterogeneity macrophages. Tumor-promoting TAMs comprise subpopulations characterized by distinct markers unique transcriptional profiles, rendering them potential targets for anticancer therapies through either depletion or reprogramming from pro-tumoral an anti-tumoral state. Single-cell RNA sequencing technology has significantly enhanced our research resolution, breaking traditional simplistic definitions macrophage subtypes deepening understanding diversity within TAMs. However, unified elucidation nomenclature molecular characteristics this remains lacking. In review, we assess application conventional polarization colorectal malignancies explore several defined single-cell omics perspective recent years, categorizing based their functions.

Language: Английский

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Endoplasmic reticulum stress-related prognosis signature characterizes the immune landscape and predicts the prognosis of colon adenocarcinoma

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: April 1, 2025

Colon adenocarcinoma (COAD) is characterized by high mortality and poor prognosis. Endoplasmic reticulum stress-related gene (ERSG) plays an indispensable role in the progression immunotherapy of COAD. In this study, we evaluated prognostic value ERSGs We constructed validated ERSG-related signature based on public databases using univariate Cox analysis, Kaplan-Meier survival LASSO method, multivariate analysis. addition, TCGA-COAD, Human Protein Atlas, quantitative real-time PCR (q-PCR) were used to detect mRNA protein expressions normal cancer tissues/cells. The immunotherapeutic cohort was evaluate predictive ERSG for sensitivity. signature, consisted HSPA1A, SERPINA1, DAPK1, could predict prognosis patients with Clinicopathologic characteristics significantly correlated risk scores. There significant differences proportion tumor-infiltrating immune cells, TP53 mutation rate, expression checkpoint-related genes, IC50 chemotherapeutic drugs between low- high-risk groups. Compared tissues, three decreased tissues. NCM460, levels HSPA1A DAPK1 majority COAD cell lines, whereas level SERPINA1 increased HCT116 SW480, reduced SW620. be as a predictor outcomes. has sensitivity COAD, helping guide personalized treatment.

Language: Английский

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Multidimensional differences of right- and left-sided colorectal cancer and their impact on targeted therapies

npj Precision Oncology, Journal Year: 2025, Volume and Issue: 9(1)

Published: April 22, 2025

Despite advances in metastatic colorectal cancer (mCRC) treatment, long-term survival remains poor, particularly right-sided (RCRC), which has a worse prognosis compared to left-sided CRC (LCRC). This disparity is driven by the complex biological diversity of these malignancies. RCRC and LCRC differ not only clinical presentation outcomes but also their underlying molecular genetic profiles. article offers detailed literature review focusing on distinctions between LCRC. We explore key differences across embryology, anatomy, pathology, omics, tumor microenvironment (TME), providing insights into how factors contribute therapeutic responses. Furthermore, we examine implications differences, considering whether conventional classification right- forms should be refined. Recent findings suggest that this binary may overlook critical complexities. Therefore, propose future approaches integrate better guide personalized treatments, especially anti-EGFR therapies, improve patient outcomes.

Language: Английский

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Survival in Stage IV Colorectal Cancer at Diagnosis

Surgical Oncology Insight, Journal Year: 2025, Volume and Issue: unknown, P. 100144 - 100144

Published: May 1, 2025

Language: Английский

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Constructing an immune-related prognostic signature for predicting prognosis and immune response in hepatocellular carcinoma

Heliyon, Journal Year: 2024, Volume and Issue: 10(13), P. e34012 - e34012

Published: July 1, 2024

BackgroundCurrently, there are few studies on immune-related prognostic analysis of hepatocellular carcinoma (HCC). Our aim was to establish an immune-correlated model for HCC.MethodsImmune-associated cells were obtained from the scRNA-seq dataset (GSE149614) HCC. Differentially expressed genes between normal and tumor immune-associated ImmPort database used identify differentially (IRDEGs). Subsequently, risk established in TCGA-LIHC cohort (n = 438) Cancer Genome Atlas (TCGA) by using Kaplan-Meier (K-M) survival curve, univariate/multivariate Cox regression analysis. we further analyzed immune microenvironment characteristics, somatic mutation, checkpoint its ligand expression levels high- low-risk groups, as well drug sensitivity prediction. ICGC set validation cohort. three independent Gene Expression Omnibus (GEO) datasets (GSE54236, GSE14520, GSE64041) verify IRDEGs expression, PCR assays clinical samples.ResultsThe composed 4 genes, namely B2M, SPP1, PPIA, HRG. The 438 HCC patients divided into group. high-risk group associated with poor prognosis, including higher T stage, advanced pathological stages, less cell infiltration, TP53 mutation rate, high CTLA4 HAVCR2. Besides, populations benefit most chemotherapy drugs. Similarly, performance validated ICGC. All four (TCGA-LIHC cohort, GSE54236, q-PCR results demonstrated that, compared samples, expressions B2M HRG lower SPP1 higher.ConclusionIn summary, signature had a good predictive prognosis immunotherapy patients.

Language: Английский

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Exploration of bacterial lipopolysaccharide-related genes signature based on T cells for predicting prognosis in colorectal cancer

Aging, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 6, 2024

The intratumoral microorganisms participates in the progression and immunotherapy of colorectal cancer (CRC). However, due to technical limitations, impact on CRC has not been fully understood. Therefore, we conducted a systematic analysis relationship between bacterial lipopolysaccharide (LPS)-associated genes immune cells explore new biomarkers for predicting prognosis CRC.

Language: Английский

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