Bone marrow mesenchymal stem cells modulate miR-202-3p to suppress neuronal apoptosis following spinal cord injury through autophagy activation via the AMPK, MAPK, and PI3K/AKT/mTOR signaling pathway DOI Creative Commons
Ke Huang, Jing Fang,

Weiming Sun

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 3, 2024

Bone marrow mesenchymal stem cells (BMMSCs) have garnered attention as promising therapeutic modalities for spinal cord injury (SCI) due to their neuroregenerative, anti-apoptotic, and functional recovery-enhancing properties. The central role of microRNAs (miRNAs) in mediating the beneficial outcomes resulting from BMMSCs SCI has been highlighted recent studies, suggesting that targeted modulation specific miRNAs holds potential augmenting recovery. Our previous investigation implicated miR-202-3p reparative processes injured cords, although precise mechanistic underpinnings remain elusive. In vivo, were administered rats, while vitro, was transfected into PC-12 cells. Motor capabilities recovery assessed via Basso-Beattie-Bresnahan (BBB) scores footprinting tests; evaluation neuronal tissue repair conducted using Nissl staining, TUNEL hematoxylin eosin (HE) immunofluorescence; impacts on cellular autophagy, apoptosis, relevant pathways evaluated Western blotting, quantitative polymerase chain reaction (qPCR), transmission electron microscopy (TEM). Functionally, utilized improve motor rats. Histopathologically, they contributed damaged cords regeneration nerve axons. At molecular level, stimulated autophagy suppressed apoptosis by regulating AMPK, MAPK, PI3K/AKT/mTOR pathway. Collectively, our findings demonstrate coordinate inhibit mTOR activation PI3K/AKT pathways, thereby promoting TFEB dephosphorylation, modulating ultimately fostering post-SCI.

Language: Английский

Advancements in Research on Mesenchymal Stem-Cell-Derived Exosomal miRNAs: A Pivotal Insight into Aging and Age-Related Diseases DOI Creative Commons
Minglei Huang,

Ye Liu,

Longze Zhang

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(11), P. 1354 - 1354

Published: Oct. 24, 2024

Mesenchymal stem cells (MSCs) are capable of differentiating into various cell types and play a crucial role in repairing aging tissues diseased organs. Aging manifests as gradual loss cellular, tissue, organ function, leading to the progression pathologies. Exosomes (Exos) extracellular vesicles secreted by cells, which maintain cellular homeostasis, clear debris, facilitate communication between This review provides comprehensive summary mechanisms for synthesis sorting MSC-Exo miRNAs summarizes current research status MSCs-Exos mitigating age-related diseases. It delves underlying molecular mechanisms, encompass antioxidative stress, anti-inflammatory response, promotion angiogenesis. Additionally, this also discusses potential challenges future strategies advancing miRNA-based therapies treatment

Language: Английский

Citations

2
A Perfect Storm: The Convergence of Aging, Human Immunodeficiency Virus Infection, and Inflammasome Dysregulation DOI Creative Commons

Siva Thirugnanam,

Namita Rout

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(5), P. 4768 - 4786

Published: May 15, 2024

The emergence of combination antiretroviral therapy (cART) has greatly transformed the life expectancy people living with HIV (PWH). Today, over 76% individuals have access to this life-saving therapy. However, progress come a new challenge: an increase in age-related non-AIDS conditions among patients HIV. These manifest earlier PWH than uninfected individuals, accelerating aging process. Like PWH, population experiences immunosenescence marked by increased proinflammatory environment. This phenomenon is linked chronic inflammation, driven part cellular structures called inflammasomes. Inflammatory signaling pathways activated HIV-1 infection play key role inflammasome formation, suggesting crucial link between and inflammatory state. review outlines processes triggered aging, focus on also explores current research regarding inflammasomes potential strategies for targeting mitigate inflammation. Further presents unique opportunity develop targeted interventions innovative therapeutic modalities combating aging-associated processes.

Language: Английский

Citations

1
Bufalin Ameliorates Myocardial Ischemia/Reperfusion Injury by Suppressing Macrophage Pyroptosis via P62 Pathway DOI Creative Commons
Chang Li,

Zhen Ma,

Xiang Wei

et al.

Journal of Cardiovascular Translational Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 28, 2024

Bufalin, which is isolated from toad venom, exerts positive effects on hearts under pathological circumstance. We aimed to investigate the and mechanisms of bufalin myocardial I/R injury. In vivo, ameliorated injury, characteristics with better ejection function, decreased infarct size less apoptosis. The levels pyroptotic proteins were increased in I/R-treated macrophages inflammatory cytokines expressed more I/R-induced mouse, could be attenuated by bufalin. Bufalin also reduced H/R-treated macrophage pyroptosis vitro. Autophagic flux blockage ROS accumulation impaired macrophages. Overexpression p62 abrogated anti-proptosis anti-oxidative apoptosis related changed TUNEL-positive ratio was raised cardiomyocytes that received conditioned medium treatment macrophages, while pretreatment reduce These findings indicate may attenuate injury suppressing via P62 pathway.

Language: Английский

Citations

1
Bone Marrow Mesenchymal Stem Cells Modulate miR-202-3p to Suppress Neuronal Apoptosis Following Spinal Cord Injury Through Autophagy Activation via the AMPK, MAPK, and PI3K/AKT/mTOR Signaling Pathway DOI Creative Commons
Ke Huang, Jing Fang,

Weiming Sun

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 2, 2024

Abstract Background: Bone marrow mesenchymal stem cells (BMMSCs) have garnered attention as promising therapeutic modalities for spinal cord injury (SCI) due to their neuroregenerative, anti-apoptotic, and functional recovery-enhancing properties. The central role of microRNAs (miRNAs) in mediating the beneficial outcomes resulting from BMMSCs SCI has been highlighted recent studies, suggesting that targeted modulation specific miRNAs holds potential augmenting recovery. Our previous investigation implicated miR-202-3p reparative processes injured cords, although precise mechanistic underpinnings remain elusive. Methods: In vivo, were administered rats, while vitro, was transfected into PC-12 cells. Motor capabilities recovery assessed via Basso-Beattie-Bresnahan (BBB) scores footprinting tests; evaluation neuronal tissue repair conducted using Nissl staining, TUNEL hematoxylin eosin (HE) immunofluorescence; impacts on cellular autophagy, apoptosis, relevant pathways evaluated Western blotting, quantitative polymerase chain reaction (qPCR), transmission electron microscopy (TEM). Results: Functionally, utilized improve motor rats. Histopathologically, they contributed damaged cords regeneration nerve axons. At molecular level, stimulated autophagy suppressed apoptosis by regulating AMPK, MAPK, PI3K/AKT/mTOR pathway. Conclusions: Collectively, our findings demonstrate coordinate inhibit mTOR activation PI3K/AKT pathways, thereby promoting TFEB dephosphorylation, modulating ultimately fostering post-SCI.

Language: Английский

Citations

0
Bone marrow mesenchymal stem cells modulate miR-202-3p to suppress neuronal apoptosis following spinal cord injury through autophagy activation via the AMPK, MAPK, and PI3K/AKT/mTOR signaling pathway DOI Creative Commons
Ke Huang, Jing Fang,

Weiming Sun

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 3, 2024

Bone marrow mesenchymal stem cells (BMMSCs) have garnered attention as promising therapeutic modalities for spinal cord injury (SCI) due to their neuroregenerative, anti-apoptotic, and functional recovery-enhancing properties. The central role of microRNAs (miRNAs) in mediating the beneficial outcomes resulting from BMMSCs SCI has been highlighted recent studies, suggesting that targeted modulation specific miRNAs holds potential augmenting recovery. Our previous investigation implicated miR-202-3p reparative processes injured cords, although precise mechanistic underpinnings remain elusive. In vivo, were administered rats, while vitro, was transfected into PC-12 cells. Motor capabilities recovery assessed via Basso-Beattie-Bresnahan (BBB) scores footprinting tests; evaluation neuronal tissue repair conducted using Nissl staining, TUNEL hematoxylin eosin (HE) immunofluorescence; impacts on cellular autophagy, apoptosis, relevant pathways evaluated Western blotting, quantitative polymerase chain reaction (qPCR), transmission electron microscopy (TEM). Functionally, utilized improve motor rats. Histopathologically, they contributed damaged cords regeneration nerve axons. At molecular level, stimulated autophagy suppressed apoptosis by regulating AMPK, MAPK, PI3K/AKT/mTOR pathway. Collectively, our findings demonstrate coordinate inhibit mTOR activation PI3K/AKT pathways, thereby promoting TFEB dephosphorylation, modulating ultimately fostering post-SCI.

Language: Английский

Citations

0