Redox homeostasis of one-carbon metabolism-dependent reprogramming is critical for RCC progression under exogenous serine/glycine-deprived conditions DOI Creative Commons
Huijuan Wang,

Mengzhen Fan,

Sichang Liu

et al.

BMC Cancer, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 18, 2024

Serine/glycine are critical for the growth and survival of cancer cells. Some cells more dependent on exogenous serine/glycine than endogenously synthesized serine/glycine. However, function underlying mechanisms in renal cell carcinoma (RCC) remain unclear. We conducted a comprehensive assessment RCC progression under conditions deprivation explored mechanism via immunofluorescence, autophagic flux analysis, extracellular acidification rate (ECAR) oxygen consumption (OCR) measurements. The expression serine synthesis pathway enzymes was decreased specimens, de novo (SSP) reduced RCC. And levels were little. Yet, significantly inhibited both vitro vivo, indicating that important progression. Mechanistically, disrupted one-carbon metabolism increased ratio NAD(P)+/NAD(P)H, resulting accumulation reactive species (ROS) oxidative stress, which induced enhanced lysosome membrane permeabilization (LMP), leading to release lysosomal cathepsins into cytoplasm, ultimately triggered death (LDCD) Our results indicate by maintaining metabolism-dependent redox homeostasis, provides new insights development dietary starvation-based therapeutic approaches

Language: Английский

Unraveling the role of ADAMs in clinical heterogeneity and the immune microenvironment of hepatocellular carcinoma: insights from single-cell, spatial transcriptomics, and bulk RNA sequencing DOI Creative Commons
Junhong Chen, Qihang Yuan,

Hewen Guan

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 13, 2024

Hepatocellular carcinoma (HCC) is a prevalent and heterogeneous tumor with limited treatment options unfavorable prognosis. The crucial role of disintegrin metalloprotease (ADAM) gene family in the microenvironment HCC remains unclear.

Language: Английский

Citations

5
Redox homeostasis of one-carbon metabolism-dependent reprogramming is critical for RCC progression under exogenous serine/glycine-deprived conditions DOI Creative Commons
Huijuan Wang,

Mengzhen Fan,

Sichang Liu

et al.

BMC Cancer, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 18, 2024

Serine/glycine are critical for the growth and survival of cancer cells. Some cells more dependent on exogenous serine/glycine than endogenously synthesized serine/glycine. However, function underlying mechanisms in renal cell carcinoma (RCC) remain unclear. We conducted a comprehensive assessment RCC progression under conditions deprivation explored mechanism via immunofluorescence, autophagic flux analysis, extracellular acidification rate (ECAR) oxygen consumption (OCR) measurements. The expression serine synthesis pathway enzymes was decreased specimens, de novo (SSP) reduced RCC. And levels were little. Yet, significantly inhibited both vitro vivo, indicating that important progression. Mechanistically, disrupted one-carbon metabolism increased ratio NAD(P)+/NAD(P)H, resulting accumulation reactive species (ROS) oxidative stress, which induced enhanced lysosome membrane permeabilization (LMP), leading to release lysosomal cathepsins into cytoplasm, ultimately triggered death (LDCD) Our results indicate by maintaining metabolism-dependent redox homeostasis, provides new insights development dietary starvation-based therapeutic approaches

Language: Английский

Citations

2