Loureirin B Accelerates Diabetic Wound Healing by Promoting TGFβ/Smad‐Dependent Macrophage M2 Polarization: A Concerted Analytical Approach Through Single‐Cell RNA Sequencing and Experimental Verification DOI
Weijing Fan, Yin Qu, Xin Yuan

et al.

Phytotherapy Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

ABSTRACT Diabetic wound (DW) represent a significant clinical challenge and often fail to heal effectively. Loureirin B (LB), flavonoid extracted from dragon's blood, has shown potential by influencing macrophage polarization promoting healing. However, its mechanisms efficacy in DW remain be explored. This study employed single‐cell RNA sequencing analyze the classification of cells diabetic foot ulcers identify related influenced macrophages. Molecular docking was used predict interactions LB with key proteins TGFβ/Smad signaling pathway. The effects on healing were further validated through vitro vivo experiments using model. Single‐cell analysis identified specific subtypes involved process highlighted role suggested action within In studies showed that under high glucose conditions, promoted pro‐inflammatory M1 healing‐promoting M2 ECM production fibroblasts activating TGF‐β/Smad signaling. vivo, treatment enhanced rates mice fibroblast synthesis regulates promote These findings suggest could therapeutic agent for improving healing, emphasizing need explore human subjects.

Language: Английский

Loureirin B Accelerates Diabetic Wound Healing by Promoting TGFβ/Smad‐Dependent Macrophage M2 Polarization: A Concerted Analytical Approach Through Single‐Cell RNA Sequencing and Experimental Verification DOI
Weijing Fan, Yin Qu, Xin Yuan

et al.

Phytotherapy Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

ABSTRACT Diabetic wound (DW) represent a significant clinical challenge and often fail to heal effectively. Loureirin B (LB), flavonoid extracted from dragon's blood, has shown potential by influencing macrophage polarization promoting healing. However, its mechanisms efficacy in DW remain be explored. This study employed single‐cell RNA sequencing analyze the classification of cells diabetic foot ulcers identify related influenced macrophages. Molecular docking was used predict interactions LB with key proteins TGFβ/Smad signaling pathway. The effects on healing were further validated through vitro vivo experiments using model. Single‐cell analysis identified specific subtypes involved process highlighted role suggested action within In studies showed that under high glucose conditions, promoted pro‐inflammatory M1 healing‐promoting M2 ECM production fibroblasts activating TGF‐β/Smad signaling. vivo, treatment enhanced rates mice fibroblast synthesis regulates promote These findings suggest could therapeutic agent for improving healing, emphasizing need explore human subjects.

Language: Английский

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