A Prognostic Riskscore Model Related to Helicobacter pylori Infection in Stomach Adenocarcinoma DOI Creative Commons

Peng Jing,

Qi Yan,

Wennan Pei

et al.

International Journal of Genomics, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Background:Helicobacter pylori (HP) is associated with the development of various stomach diseases, one major risk factors for adenocarcinoma (STAD). Methods: The HP infection score between tumor and normal groups was compared by single-sample gene set enrichment analysis (ssGSEA). key modules related to were identified weighted coexpression network (WGCNA), functional conducted on these module genes. Further, limma package used screen differentially expressed genes (DEGs) HP-positive HP-negative STAD. prognostic obtained construct riskscore model, performance model validated. correlation immune microenvironment (TIME) analyzed Spearman's method. single-cell atlas STAD delineated. mRNA expression levels verified using cells, migration invasion capacities cells evaluated wound healing assay transwell assay. Results: in group significantly higher than that group. purple royal blue showed STAD, enriched immune-related pathway. five (CTLA4, CPVL, EMB, CXCR4, FAM241A) screened from infection-related DEGs, which utilized establishing good robustness. Riskscore exhibited strong TIME Single-cell revealed CXCR4 highly Epithelial Cell 1, 2, parietal CTLA4, FAM241A, high silencing CPVL could suppress cells. Conclusion: This study established a based genes, provide reference prediction treatment targets

Language: Английский

A Prognostic Riskscore Model Related to Helicobacter pylori Infection in Stomach Adenocarcinoma DOI Creative Commons

Peng Jing,

Qi Yan,

Wennan Pei

et al.

International Journal of Genomics, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Background:Helicobacter pylori (HP) is associated with the development of various stomach diseases, one major risk factors for adenocarcinoma (STAD). Methods: The HP infection score between tumor and normal groups was compared by single-sample gene set enrichment analysis (ssGSEA). key modules related to were identified weighted coexpression network (WGCNA), functional conducted on these module genes. Further, limma package used screen differentially expressed genes (DEGs) HP-positive HP-negative STAD. prognostic obtained construct riskscore model, performance model validated. correlation immune microenvironment (TIME) analyzed Spearman's method. single-cell atlas STAD delineated. mRNA expression levels verified using cells, migration invasion capacities cells evaluated wound healing assay transwell assay. Results: in group significantly higher than that group. purple royal blue showed STAD, enriched immune-related pathway. five (CTLA4, CPVL, EMB, CXCR4, FAM241A) screened from infection-related DEGs, which utilized establishing good robustness. Riskscore exhibited strong TIME Single-cell revealed CXCR4 highly Epithelial Cell 1, 2, parietal CTLA4, FAM241A, high silencing CPVL could suppress cells. Conclusion: This study established a based genes, provide reference prediction treatment targets

Language: Английский

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