Vavilov Journal of Genetics and Breeding, Journal Year: 2020, Volume and Issue: 24(6), P. 636 - 642
Published: Oct. 28, 2020
The free-living flatworm Macrostomum mirumnovem is a neopolyploid species whose genome underwent recent Whole Genome Duplication (WGD). In the result of chromosome fusions ancient haploid set, large metacentric chromosomes were formed. addition to three pairs small metacentrics, current karyotype M. contains two chromosomes, MMI1 and MMI2. generation microdissected DNA libraries enriched for repeats followed by probe preparation fluorescent in situ hybridization (FISH) performed. probes obtained marked regions different chromosomes. size localization these varied copies They even homologous suggesting their divergence due re-diploidization after WGD. Besides newly formed repeats, B found karyotypes studied specimens mirumnovem. These morphology. FISH with revealed that some Bs had distinct content. could paint differently worms same sample. carry bright specific signal or show no at all. latter cases, be absent pericentromeric region chromosome. Possible mechanisms formation further evolution are discussed. results indicate an important role repetitive DNAs play initiating rapid differentiation copies. Taking together, peculiarities (a high level intraspecific karyotypic diversity associated number variation, structural chromosomal rearrangements, new repeats) phenotypic features (small body size, short lifecycle, easy maintenance laboratory) make this perspective model studies genomic passed through WGD event.
Language: Английский