In
macroecology,
the
concept
of
r-
and
K-strategy
has
been
widely
applied,
yet,
there
have
limited
studies
on
microbial
life-history
strategies
in
temperate
grasslands
using
multiple
sequencing
approaches.
Total
phospholipid
fatty
acid
(PLFA)
analysis,
high-throughput
meta-genomic
sequencing,
GeoChip
technologies
were
used
to
examine
changes
traits
a
chronosequence
restored
(1,
5,
10,
15,
25,
30
years
since
restoration).
Grassland
restoration
increased
relative
abundances
Actinobacteria,
Proteobacteria,
Bacteroidetes
but
reduced
Acidobacteria,
Planctomycetes,
Chloroflexi.
PLFA
analysis
revealed
that
grassland
fungi:bacteria
Gram-positive:Gram-negative
bacteria
ratios.
Combined
with
data,
we
found
shifted
microorganisms
from
oligotrophic
(K-)
copiotrophic
(r-)
groups,
consistent
rRNA
operon
copy
number
community.
Structural
equation
modeling
showed
soil
properties
positively
(
In
macroecology,
the
concept
of
r-
and
K-strategy
has
been
widely
applied,
yet,
there
have
limited
studies
on
microbial
life-history
strategies
in
temperate
grasslands
using
multiple
sequencing
approaches.
Total
phospholipid
fatty
acid
(PLFA)
analysis,
high-throughput
meta-genomic
sequencing,
GeoChip
technologies
were
used
to
examine
changes
traits
a
chronosequence
restored
(1,
5,
10,
15,
25,
30
years
since
restoration).
Grassland
restoration
increased
relative
abundances
Actinobacteria,
Proteobacteria,
Bacteroidetes
but
reduced
Acidobacteria,
Planctomycetes,
Chloroflexi.
PLFA
analysis
revealed
that
grassland
fungi:bacteria
Gram-positive:Gram-negative
bacteria
ratios.
Combined
with
data,
we
found
shifted
microorganisms
from
oligotrophic
(K-)
copiotrophic
(r-)
groups,
consistent
rRNA
operon
copy
number
community.
Structural
equation
modeling
showed
soil
properties
positively
(