Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: unknown, P. 117090 - 117090
Published: Dec. 1, 2024
Language: Английский
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 9, 2025
Early and precise diagnosis of neurodegenerative disorders like Alzheimer's (AD) Parkinson's (PD) is crucial for slowing their progression enhancing patient outcomes. Exosomal microRNAs (miRNAs) are emerging as promising biomarkers due to ability reflect the diseases' pathology, yet low abundance poses significant detection hurdles. This review article delves into burgeoning field electrochemical biosensors, designed exosomal miRNA biomarkers. Electrochemical biosensors offer a compelling solution, combining sensitivity required detect low-abundance with specificity needed discern profiles distinctive neural pathological states. We explore operational principles these including transduction mechanisms that facilitate detection. The also summarizes advancements in nanotechnology, signal enhancement, bioreceptor anchoring, microfluidic integration improve sensor accuracy. evidence use disease analyzed, focusing on clinical impact, diagnostic precision, obstacles faced practical applications. Their potential point-of-care testing regulatory considerations market entry discussed. Looking toward future, highlights forthcoming innovations might revolutionize early processes. impressive sensitivity, specificity, compatibility, track become instrumental diseases, possibly transforming care prognosis.
Language: Английский
Citations
2
Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 268, P. 116878 - 116878
Published: Nov. 1, 2024
Conventional point-of-care lateral flow immunoassays are characterized by an antibody-tagged probe irregular coupling that can limit sensitivity and require a long assay's time. We synthesized polyethylene glycol-modified selenium nanoparticles (PEG-SeNPs) template method developed strategy to set antibody probes targeted orderly using PEG-SeNPs. Synthesized PEG-SeNPs with high stability could immobilize antibodies in the "stand-up" orientation, resulting faster detection time of less than 1 min direct observer visualization without instruments or equipment. Results showed SARS-CoV-2 be systematically structured on chip, 10 pg/mL, significantly higher conventional chips. The new device has been validated 192 clinical samples we found 100% negative coincidence, 93.94% positive 95.83% overall coincidence reverse transcriptional PCR test. arrayed probe's allowed detect throat swabs, saliva, serum, fingertip blood samples, mutant strains cross-reactivity common respiratory viruses pathogenic strains, demonstrating promising potential for universal colorimetric platform ultrafast field-deployable diagnostics.
Language: Английский
Citations
5
Aggregate, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 13, 2025
ABSTRACT Urogenital system tumors include prostate cancer, bladder ovarian and other very common solid tumor diseases with high morbidity mortality. The unique physiological anatomical features of the urogenital render it particularly amenable to application tissue imaging techniques for diagnostic purposes. advancement aggregation‐induced emission (AIE) materials has addressed limitations associated conventional fluorescent that are prone aggregation‐caused quenching. This facilitated development innovative AIE characterized by enhanced photostability, an increased signal‐to‐noise ratio, improved quality. article reviews research progress biosensors in diagnosis tumors. It mainly involves biomarker vitro fluorescence such as uterine which based on biosensors. In addition, a comprehensive description biosensors’ synthesis strategies is provided. includes detailed elucidation platforms intracellular mechanisms basic principles AIE, accompanied presentation quantitative analysis cell results. limitations, challenges suggestions field summarized, prospect prospected. tumors, also provides catalyst exploring characteristics its wide disease diagnosis.
Language: Английский
Citations
0
Biosensors, Journal Year: 2025, Volume and Issue: 15(4), P. 203 - 203
Published: March 21, 2025
Ovarian cancer (OC) is one of the leading causes gynecological cancer-related death worldwide. Late diagnosis at advanced stages OC reason for a higher mortality rate. Earlier and proper treatment are important improving prognosis patients. Biosensors offer accurate, low-cost, rapid, user-friendly devices that can be employed detection OC-specific biomarkers in early stage. Therefore, it to consider potential biological fluids confirm prognosis. Out many biomarkers, most commonly tested clinically antigen 125 (CA-125). However, CA-125 considered poor biomarker diagnosis. Several biosensing methods were developed sensitive quantitative each biomarker. In abnormal expression patients, nucleic acids, enzymes, cells, exosomes used as target construction biosensors. This review focuses on development various using multiple methods. Here, we describe origin significance OC-associated working principle biosensors, classification biosensors based their recognition elements signal transducers. The modes sensitivity sensors discussed. Finally, challenges fabrication, obstacles clinical application, future prospects
Language: Английский
Citations
0
ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown
Published: May 14, 2025
Extracellular vesicles are nanoscale released by a diversity of cells that mediate intercellular communication transporting an array biomolecules. They gaining increasing attention in cancer research due to their ability carry specific biomarkers. This characteristic makes them potentially useful for highly sensitive, noninvasive diagnostic procedures and more precise prognostic assessments. Consequently, EVs emerging as transformative tool treatment, facilitating early detection personalized medicine. Despite significant progress, clinical implementation is hindered challenges EV isolation, purification, characterization. However, developing advanced biosensor technologies offers promising solutions these obstacles. review highlights recent progress biosensors analysis, focusing on various sensing modalities including optical, electrochemical, microfluidic, nanomechanical, biological sensors. We also explore techniques characterization, such electron microscopy, atomic force nanoparticle tracking single-particle analysis. Furthermore, the critically assesses associated with put forward future directions, aiming usher cutting-edge era precision medicine through advanced, sensor-based, diagnosis detecting EV-carried
Language: Английский
Citations
0
Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: unknown, P. 117090 - 117090
Published: Dec. 1, 2024
Language: Английский
Citations
1