Comparative Examination of Feline Coronavirus and Canine Coronavirus Effects on Extracellular Vesicles Acquired from A-72 Canine Fibrosarcoma Cell Line DOI Creative Commons

Sandani V. T. Wijerathne,

Rachana Pandit, Chioma C. Ezeuko

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(5), P. 477 - 477

Published: May 15, 2025

Introduction: Coronavirus (CoV) is an extremely contagious, enveloped positive-single-stranded RNA virus, which has become a global pandemic that causes several illnesses in humans and animals. Hence, it necessary to investigate viral-induced reactions across diverse hosts. Herein, we propose utilizing naturally secreted extracellular vesicles (EVs), mainly focusing on exosomes examine virus–host responses following CoV infection. Exosomes are small membrane-bound originating from the endosomal pathway, play pivotal role intracellular communication physiological pathological processes. We suggested could impact EV formation, content, immune vitro. Methods: In this study, infected A-72, canine fibroblast cell line, with feline coronavirus (FCoV) (CCoV) independently exosome-free media at 0.001 multiplicity of infection (MOI), incubation periods 48 72 h. The viability was significantly downregulated increased time FCoV CCoV infection, identified by performing 3-(4,5-dimethylthiazo-1-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. After EVs were isolated through ultracentrifugation, subsequent analysis involved quantifying characterizing purified using various techniques. Results: NanoSight particle tracking (NTA) verified dimensions fell between 100 200 nm both periods. At periods, total protein levels upregulated A-72-derived infections. However, DNA gradually time. Dot blot indicated expression ACE2, IL-1β, Flotillin-1, CD63, caspase-8, Hsp90 modified Conclusions: Our results infections modulate production development viral diseases. Investigating animal will provide in-depth insight into host exosome biology during our findings contribute comprehension characterization interactions

Language: Английский

New Challenges and Opportunities: Extracellular Vesicles in Biological and Biochemical Processes DOI Open Access
Ilaria Giusti, Celeste Caruso Bavisotto

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3395 - 3395

Published: April 4, 2025

Cell-to-cell communication plays a crucial role in many processes, both physiological and pathological assets [...].

Language: Английский

Citations

0
Integrated Single Nanoparticle Analysis for Rapid Quantification of Spatiotemporal Crosstalk between Herpes Simplex Virus-1 and Extracellular Vesicles DOI
Jiaxin Luo,

Ai Lun Yang,

Siyao Wang

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: May 9, 2025

Cells secrete extracellular vesicles (EVs) to mediate precise communication during viral infections, yet the spatiotemporal regulation of EV composition by herpes simplex virus 1 (HSV-1) remains poorly understood. Here, we develop an integrated single-nanoparticle analysis platform combining nanoporous membrane-based isolation with on-chip immunoassay quantitatively probe EV-HSV-1 interplay throughout infection. A dual-membrane filter design significantly enhances nanoparticle recovery, enabling high-sensitivity single-particle detection. We reveal that HSV-1-infected neural stem cells display glycoprotein B on surfaces at early stage (<8 hpi), while intact virions are selectively packaged into EVs later (24-48 hpi). Proteomic profiling indicates infected cell-derived facilitate antigen processing and presentation, potentially amplifying antiviral responses. Functional studies further demonstrate promote entry late stages (48 likely via EV-virion encapsulation. These findings elucidate a dynamic EV-virus interplay, offering insights HSV-1 pathogenesis EV-mediated immune modulation. Our provides transformative approach for advancing infection diagnostics therapeutics.

Language: Английский

Citations

0
Comparative Examination of Feline Coronavirus and Canine Coronavirus Effects on Extracellular Vesicles Acquired from A-72 Canine Fibrosarcoma Cell Line DOI Creative Commons

Sandani V. T. Wijerathne,

Rachana Pandit, Chioma C. Ezeuko

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(5), P. 477 - 477

Published: May 15, 2025

Introduction: Coronavirus (CoV) is an extremely contagious, enveloped positive-single-stranded RNA virus, which has become a global pandemic that causes several illnesses in humans and animals. Hence, it necessary to investigate viral-induced reactions across diverse hosts. Herein, we propose utilizing naturally secreted extracellular vesicles (EVs), mainly focusing on exosomes examine virus–host responses following CoV infection. Exosomes are small membrane-bound originating from the endosomal pathway, play pivotal role intracellular communication physiological pathological processes. We suggested could impact EV formation, content, immune vitro. Methods: In this study, infected A-72, canine fibroblast cell line, with feline coronavirus (FCoV) (CCoV) independently exosome-free media at 0.001 multiplicity of infection (MOI), incubation periods 48 72 h. The viability was significantly downregulated increased time FCoV CCoV infection, identified by performing 3-(4,5-dimethylthiazo-1-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. After EVs were isolated through ultracentrifugation, subsequent analysis involved quantifying characterizing purified using various techniques. Results: NanoSight particle tracking (NTA) verified dimensions fell between 100 200 nm both periods. At periods, total protein levels upregulated A-72-derived infections. However, DNA gradually time. Dot blot indicated expression ACE2, IL-1β, Flotillin-1, CD63, caspase-8, Hsp90 modified Conclusions: Our results infections modulate production development viral diseases. Investigating animal will provide in-depth insight into host exosome biology during our findings contribute comprehension characterization interactions

Language: Английский

Citations

0