Leveraging Organ‐on‐Chip Models to Investigate Host–Microbiota Dynamics and Targeted Therapies for Inflammatory Bowel Disease DOI Creative Commons
Tim Kaden, Raquel Alonso‐Román, Johannes Stallhofer

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 3, 2024

Abstract Inflammatory bowel disease (IBD) is an idiopathic gastrointestinal with drastically increasing incidence rates. Due to its multifactorial etiology, a precise investigation of the pathogenesis extremely difficult. Although reductionist cell culture models and more complex in animals have clarified understanding individual mechanisms contributing factors IBD past, it remains challenging bridge research clinical practice. Conventional 2D cannot replicate host–microbiota interactions stable long‐term microbial culture. Further, extrapolating data from animal patients due genetic environmental diversity leading differences immune responses. Human intestine organ‐on‐chip (OoC) emerged as alternative vitro model approach investigate IBD. OoC not only recapitulate human intestinal microenvironment accurately than cultures yet may also be advantageous for identification important disease‐driving pharmacological interventions targets possibility emulating different complexities. The predispositions biological hallmarks focusing on at mucosal barrier are elucidated here. Additionally, potential OoCs explore microbiota‐related therapies personalized medicine treatment discussed.

Language: Английский

Circulating B vitamins metabolites in depressive disorders - connections with the microbiota-gut-brain axis DOI

Wiktor Śliwiński,

Oliwia Gawlik-Kotelnicka

Behavioural Brain Research, Journal Year: 2024, Volume and Issue: 472, P. 115145 - 115145

Published: July 9, 2024

Language: Английский

Citations

10

Microbiome dysbiosis in SARS-CoV-2 infection: implication for pathophysiology and management strategies of COVID-19 DOI Creative Commons
Shukur Wasman Smail,

Niaz Albarzinji,

Rebaz Hamza Salih

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 22, 2025

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent disease 2019 (COVID-19), in late initiated a global health crisis marked by widespread infection, significant mortality, and long-term implications. While SARS-CoV-2 primarily targets system, recent findings indicate that it also significantly disrupts human microbiome, particularly gut microbiota, contributing to severity, systemic inflammation, immune dysregulation, increased susceptibility secondary infections chronic conditions. Dysbiosis, or microbial imbalance, exacerbates clinical outcomes COVID-19 has been linked long-COVID, condition affecting proportion survivors manifesting with over 200 symptoms across multiple organ systems. Despite growing recognition microbiome alterations COVID-19, precise mechanisms which interacts influences progression remain poorly understood. This narrative review investigates impact on host-microbiota dynamics evaluates its implications severity for developing personalized therapeutic strategies COVID-19. Furthermore, highlights dual role modulating progression, as promising target advancing diagnostic, prognostic, approaches managing

Language: Английский

Citations

0

Elevated risk of adverse effects from foodborne contaminants and drugs in inflammatory bowel disease: a review DOI Creative Commons
Tom Walraven, Mathias Busch, Jingxuan Wang

et al.

Archives of Toxicology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Abstract The global burden of Inflammatory bowel disease (IBD) has been rising over the last decades. IBD is an intestinal disorder with a complex and largely unknown etiology. characterized by chronically inflamed gastrointestinal tract, intermittent phases exacerbation remission. This compromised barrier can contribute to, enhance, or even enable toxicity drugs, food-borne chemicals particulate matter. review discusses whether prevalence in our society warrants consideration patients as specific population group toxicological safety assessment. Various vivo, ex vivo vitro models are discussed that simulate hallmarks may be used to study effects prevalent inflammation on hazards these various toxicants. In conclusion, risk assessments based healthy individuals not sufficiently cover patient it suggested consider this susceptible subgroup future assessments.

Language: Английский

Citations

3

Intestinal Cells-on-Chip for Permeability Studies DOI Creative Commons

Marit Keuper-Navis,

Hossein Eslami Amirabadi, Joanne M. Donkers

et al.

Micromachines, Journal Year: 2024, Volume and Issue: 15(12), P. 1464 - 1464

Published: Nov. 30, 2024

Background: To accurately measure permeability of compounds in the intestine, there is a need for preclinical vitro models that represent specificity, integrity and complexity human small intestinal barrier. Intestine-on-chip systems hold considerable promise as testing platforms, but several characteristics still require optimization further development. Methods: An established intestine-on-chip model tissue explants was adopted cell monolayer culture. A 3D-printed culture disc designed to allow static conditions subsequent studies dynamic environment. Membrane standardized read-outs were investigated compared traditional under conditions. Results: By starting cultures outside chip conventional wells plates, new design could support accurate formation both Caco-2 enteroids. When transferred with laminar flow, detection barrier (FD4 Cascade Blue) (atenolol/antipyrine). Both flow membrane had significant impact on outcomes. Conclusions: This novel cell-on-chip system offers large flexibility studies, although it requires validation more reveal its full potential.

Language: Английский

Citations

0

Leveraging Organ‐on‐Chip Models to Investigate Host–Microbiota Dynamics and Targeted Therapies for Inflammatory Bowel Disease DOI Creative Commons
Tim Kaden, Raquel Alonso‐Román, Johannes Stallhofer

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 3, 2024

Abstract Inflammatory bowel disease (IBD) is an idiopathic gastrointestinal with drastically increasing incidence rates. Due to its multifactorial etiology, a precise investigation of the pathogenesis extremely difficult. Although reductionist cell culture models and more complex in animals have clarified understanding individual mechanisms contributing factors IBD past, it remains challenging bridge research clinical practice. Conventional 2D cannot replicate host–microbiota interactions stable long‐term microbial culture. Further, extrapolating data from animal patients due genetic environmental diversity leading differences immune responses. Human intestine organ‐on‐chip (OoC) emerged as alternative vitro model approach investigate IBD. OoC not only recapitulate human intestinal microenvironment accurately than cultures yet may also be advantageous for identification important disease‐driving pharmacological interventions targets possibility emulating different complexities. The predispositions biological hallmarks focusing on at mucosal barrier are elucidated here. Additionally, potential OoCs explore microbiota‐related therapies personalized medicine treatment discussed.

Language: Английский

Citations

0