Grape berry native yeast microbiota: advancing trends in the development of sustainable vineyard pathogen biocontrol strategies

OENO One, Journal Year: 2024, Volume and Issue: 58(1)

Published: Jan. 9, 2024

Grape berry is an ecological niche for a myriad of microbes, whose interactions with one another modulate fruit health and can play role in fermentation, imparting character distinctiveness to wines. With the growing concerns about awareness risks associated overuse chemical pesticides viticulture, microbial-based pest control increasingly encouraged as more sustainable environmentally friendly strategy. The use yeasts from grape berries promising alternative vineyard diseases their increasing acceptance rapidly changing our perception fungicides. In this paper, we provide overview latest methodologies characterising dynamics context disease management, discuss prospects effective biocontrol agents viticulture. Most research has focused on rots produced by Botrytis, Aspergillus, Colletotrichum Penicillium spp. using formulations that comprise single strains specific yeast genera, including Aureobasidium, Metschnikowia, Saccharomyces, Pichia, Candida Rhodotorula. However, challenging disparity between successful outcomes obtained vitro low applicability field major limitation large-scale implementation these strategies. Novel approaches maximising stability efficiency yeast-derived bioactives are discussed review.

Language: Английский

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On the Way to the Technological Development of Newly Selected Non-Saccharomyces Yeasts Selected as Innovative Biocontrol Agents in Table Grapes

Microorganisms, Journal Year: 2024, Volume and Issue: 12(2), P. 340 - 340

Published: Feb. 6, 2024

Post-harvest decay of fresh table grapes causes considerable annual production losses. The main fungal agents both in pre- and post-harvest are B. cinerea, Penicillium spp., Aspergillus Alternaria Cladosporium spp. To date, the use agrochemicals SO2 methods to control grape molds postharvest, respectively. Significant improvements, however, have already been made apply innovative more environmentally sustainable strategies, such as Biological Control Agents (BCAs), which can reduce disease severity post-harvest. In this study, 31 new non-Saccharomyces yeast strains, isolated from berries native Apulian genotypes, were tested for their vivo effectiveness against grey mold grapes, resulting two St. bacillaris (‘N22_I1’ ‘S13_I3’), one S. diversa (‘N22_I3’), A. pullulans (‘OLB_9.1_VL’) H. uvarum (‘OLB_9.1_BR’) strains that marked efficient good BCAs. Their mechanisms action characterized through vitro assays, additional characteristics evaluated assess economic feasibility viability future technological employment. was by reducing working concentration, antagonistic effect on a wide range pathogens, ability survive formulations with long shelf life, safety human health.

Language: Английский

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Combined omics approaches expose metabolite-microbiota correlations in grape berries of three cultivars of Douro wine region

Food Chemistry, Journal Year: 2023, Volume and Issue: 429, P. 136859 - 136859

Published: July 13, 2023

Language: Английский

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Endophytic Diversity in Vitis vinifera with Different Vineyard Managements and Vitis sylvestris Populations from Northern Italy: A Comparative Study of Culture-Dependent and Amplicon Sequencing Methods

Biology, Journal Year: 2025, Volume and Issue: 14(3), P. 293 - 293

Published: March 14, 2025

This study aimed to investigate the endophytic microbial populations associated with wild and domesticated grapevines using both culture-based culture-independent methods. Through methods, 148 endophytes were identified. The dominant fungal species included Aureobasidium pullulans, Alternaria alternata, Cladosporium allicinum, while predominant bacterial Ralstonia pikettii, Nocardia niigatensis, Sphingomonas echinoides. Culture-independent methods employed metagenomic techniques explore biodiversity, focusing on targeted amplification of 16S rRNA as well ITS 26S gene regions. main identified Halomonas sp., sp. Massilia whereas population was dominated by Malassezia Mucor findings revealed that vineyard management practices did not lead statistically significant variations in communities. consistent presence these genera across all samples suggests they are stable components grapevine microbiota, remaining relatively unaffected external environmental factors.

Language: Английский

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From winery by-product to soil improver? – A comprehensive review of grape pomace in agriculture and its effects on soil properties and functions

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 982, P. 179611 - 179611

Published: May 14, 2025

Grape pomace (GP), a by-product of winemaking, is rich in organic carbon and nutrients, offering potential as an alternative to synthetic soil amendments. However, its broader use agriculture remains limited due uncertainties about long-term environmental agronomic impacts. This review assesses the GP amendment, highlighting ability enhance matter, nutrient availability, physicochemical properties. At same time, concerns remain regarding acidic nature, wide carbon-to‑nitrogen (C/N) ratio, bioactive compounds, such mycotoxins (poly)phenols, which could negatively impact microbial communities cycling. Furthermore, residual contaminants pesticides heavy metals may pose ecotoxicological risks, potentially disrupting ecosystem functions contaminating surrounding environments. Besides these challenges, research on efficiency, fate mobility soil, particularly relation type, climate, agricultural practices, limited. effects various (pre)treatments (e.g., composting, fermentation) properties interactions require more systematic investigation. Future should focus field trials, advanced analytical methods, effective monitoring frameworks. It essential refine regulatory guidance based comprehensive risk assessments ensure safe application maximize GP's benefits. Overcoming challenges transform into valuable resource for sustainable agriculture, contributing health, climate resilience, circular economy.

Language: Английский

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A comparison of microbiota isolation methods reveals habitat preferences for fermentative yeasts and plant pathogenic fungi in the grape berry

Food Microbiology, Journal Year: 2023, Volume and Issue: 118, P. 104408 - 104408

Published: Oct. 23, 2023

Language: Английский

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From the vineyard soil to the grape berry surface: Unravelling the dynamics of the microbial terroir

Agriculture Ecosystems & Environment, Journal Year: 2024, Volume and Issue: 374, P. 109145 - 109145

Published: July 9, 2024

Language: Английский

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Dynamic Succession of Natural Microbes during the Ecolly Grape Growth under Extremely Simplified Eco-Cultivation

Foods, Journal Year: 2024, Volume and Issue: 13(10), P. 1580 - 1580

Published: May 18, 2024

The composition and continuous succession of natural microbial communities during grape growth play important roles in health flavor quality as well characterizing the regional wine terroir. This study explored diversity dynamics fruit epidermal microbes at each developmental stage Ecolly grapes under an extremely simplified eco-cultivation model, analyzed interactions associations weather parameters to specific communities, emphasized metabolic functional characteristics microecology. results indicated that community changed significantly phase. dominant fungal genera mainly included

Language: Английский

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Pesticides and winemaking: A comprehensive review of conventional and emerging approaches

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2024, Volume and Issue: 23(5)

Published: Aug. 8, 2024

Abstract The use of pesticides in viticulture may play a crucial role ensuring the health and quality grapes. This review analyzes most common used, illustrating their classification toxicity, variations throughout winemaking process. Fungicides are generally harmless or mildly toxic, whereas insecticides classified as either highly moderately hazardous. Potential alternatives to synthetic wine production also reviewed, thereby including biopesticides biological agents. Analytical methods for detecting quantifying pesticide residues then described, liquid chromatography gas coupled with mass spectrometry. discusses impact process on content. Pesticides strong hydrophobicity were more likely accumulate solid byproducts, hydrophilic distributed phase. Grape's skin contains lipids, so hydrophobic adsorb strongly grape surfaces clarification has been shown be effective reduction compounds. Therefore, final could have quantities pesticides. Alcoholic fermentation dissipation. However, an antagonistic effect yeasts, affecting safety products. proteomic genomic analyses yeast growth reviewed understand effects during fermentation. last section describes new used removing from grapes wine, improving product reducing harmful effects.

Language: Английский

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Volatilomics of interactions between native yeasts and grapevine cultivars reveals terroir specificities in wines from Douro region

Food Bioscience, Journal Year: 2024, Volume and Issue: 62, P. 105463 - 105463

Published: Nov. 13, 2024

Language: Английский

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