Adaptation during the transition fromOphiocordycepsentomopathogen to insect associate is accompanied by gene loss and intensified selection DOI Creative Commons
Christopher M. Ward, Cristóbal A. Onetto, Anthony R. Borneman

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 8, 2024

Abstract Fungal and bacterial symbiosis is an important adaptation that has occurred within many insect species, which usually results in the relaxation of selection across symbiont genome. However, evolutionary pressures genomic consequences associated with this transition are not well understood. Pathogenic fungi genus Ophiocordyceps have undergone multiple, independent transitions from pathogen to associate, infecting soft-scale insects trans-generationally without killing them. To gain understanding adaptations underlying transition, long-read sequencing was utilized assemble genomes both Parthenolecanium corni its associate a single insect. A highly contiguous haploid assembly obtained for Part. , representing first Coccoidea insect, 97% 227.8 Mb genome contained 24 contigs. Metagenomic-based binning produced chromosome-level ’s associate. The 524 gene loss events compared free-living pathogenic relatives, predicted roles hyphal growth, cell wall integrity, metabolism, regulation toxin production. Contrasting patterns were observed between nuclear mitochondrial specific lineage. Intensified most frequently orthologs, while on genes found be relaxed. Furthermore, scans diversifying identified three adjacent enzymes catalyzing acetoacetate’s metabolism acetyl-COA. This work provides insight into adaptive landscape during life history, along base future research mechanisms underpinning evolution .

Language: Английский

Adaptation during the transition fromOphiocordycepsentomopathogen to insect associate is accompanied by gene loss and intensified selection DOI Creative Commons
Christopher M. Ward, Cristóbal A. Onetto, Anthony R. Borneman

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 8, 2024

Abstract Fungal and bacterial symbiosis is an important adaptation that has occurred within many insect species, which usually results in the relaxation of selection across symbiont genome. However, evolutionary pressures genomic consequences associated with this transition are not well understood. Pathogenic fungi genus Ophiocordyceps have undergone multiple, independent transitions from pathogen to associate, infecting soft-scale insects trans-generationally without killing them. To gain understanding adaptations underlying transition, long-read sequencing was utilized assemble genomes both Parthenolecanium corni its associate a single insect. A highly contiguous haploid assembly obtained for Part. , representing first Coccoidea insect, 97% 227.8 Mb genome contained 24 contigs. Metagenomic-based binning produced chromosome-level ’s associate. The 524 gene loss events compared free-living pathogenic relatives, predicted roles hyphal growth, cell wall integrity, metabolism, regulation toxin production. Contrasting patterns were observed between nuclear mitochondrial specific lineage. Intensified most frequently orthologs, while on genes found be relaxed. Furthermore, scans diversifying identified three adjacent enzymes catalyzing acetoacetate’s metabolism acetyl-COA. This work provides insight into adaptive landscape during life history, along base future research mechanisms underpinning evolution .

Language: Английский

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