Development of Duplex LAMP Technique for Detection of Porcine Epidemic Diarrhea Virus (PEDV) and Porcine Circovirus Type 2 (PCV 2) DOI Creative Commons
Supatra Areekit,

Pongbun Tangjitrungrot,

Sintawee Khuchareontaworn

et al.

Current Issues in Molecular Biology, Journal Year: 2022, Volume and Issue: 44(11), P. 5427 - 5439

Published: Nov. 3, 2022

Porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 2 (PCV2) are both important global pathogenic viruses which have a significant impact on the swine industry. In this study, duplex loop-mediated isothermal amplification (duplex LAMP) method was developed in combination with lateral flow dipstick (LFD) for simultaneous detection of PEDV PCV2 using specific sets primers probes designed based conserved regions spike gene (KF272920) an ORF (EF493839), respectively. The limit (LOD) values LAMP-LFD were 0.1 ng/µL 0.246 ng/µL, LOD 10-times more sensitive than conventional PCR RT-PCR-agarose gel-electrophoresis (PCR-AGE RT-PCR-AGE). No cross-reaction to each other that can infect pigs observed according analytical specificity tests. co-infection could be completed within approximately 1.5 h, only simple heating block required amplification. preliminary validation 50 clinical samples positive negative and/or revealed sensitivity, specificity, accuracy all 100% comparison RT-PCR. Hence, study suggests is promising tool early initial screening PCV2, beneficial prevention, planning, epidemiological surveys these diseases.

Language: Английский

Overview of Diagnostic Methods, Disease Prevalence and Transmission of Mpox (Formerly Monkeypox) in Humans and Animal Reservoirs DOI Creative Commons
Ravendra P. Chauhan, Ronen Fogel, Janice Limson

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(5), P. 1186 - 1186

Published: April 30, 2023

Mpox—formerly monkeypox—is a re-emerging zoonotic virus disease, with large numbers of human cases reported during multi-country outbreaks in 2022. The close similarities clinical symptoms that Mpox shares many orthopoxvirus (OPXV) diseases make its diagnosis challenging, requiring laboratory testing for confirmation. This review focuses on the diagnostic methods used detection naturally infected humans and animal reservoirs, disease prevalence transmission, signs, currently known host ranges. Using specific search terms, up to 2 September 2022, we identified 104 relevant original research articles case reports from NCBI-PubMed Google Scholar databases inclusion study. Our analyses observed molecular identification techniques are overwhelmingly being current diagnoses, especially real-time PCR (3982/7059 cases; n = 41 studies) conventional (430/1830 30 approaches most-frequently-used diagnose humans. Additionally, genomes, using qPCR and/or coupled genome sequencing methods, offered both reliable epidemiological evolving strains; emergence transmission novel clade ‘hMPXV-1A’ lineage B.1 2022 globally. While few serologic assays, such as ELISA, OPXV- Mpox-specific IgG (891/2801 17 IgM antibodies (241/2688 11 studies), hemagglutination inhibition (HI) detected samples (88/430 6 most other immunographic assays were OPXV-specific. Interestingly, isolation (228/1259 24 electron microscopy (216/1226 18 immunohistochemistry (28/40; 7 remain useful select instances tissue samples. In animals, Mpox-DNA various species nonhuman primates, rodents, shrews, opossums, dog, pig. With dynamics Mpox, information rapid is critical management.

Language: Английский

Citations

30
Revisiting Porcine Circovirus Infection: Recent Insights and Its Significance in the Piggery Sector DOI Creative Commons
Hemanta Kumar Maity,

Kartik Samanta,

Rajib Deb

et al.

Vaccines, Journal Year: 2023, Volume and Issue: 11(8), P. 1308 - 1308

Published: July 31, 2023

Porcine circovirus (PCV), a member of the Circoviridae family within genus Circovirus, poses significant economic risk to global swine industry. PCV2, which has nine identified genotypes (a–i), emerged as predominant genotype worldwide, particularly PCV2d. PCV2 been commonly found in both domestic pigs and wild boars, sporadically non-porcine animals. The virus spreads among populations through horizontal vertical transmission routes. Despite availability commercial vaccines for controlling porcine infections associated diseases, continuous genotypic shifts from b, subsequently b d, have maintained pathogen with substantial implications. This review aims provide an updated understanding biology, genetic variation, distribution, preventive strategies concerning circoviruses their diseases swine.

Language: Английский

Citations

17
Genotype Shift of Malaysian Porcine Circovirus 2 (PCV2) from PCV2b to PCV2d within a Decade DOI Creative Commons
Chew Yee Tan, Roongroje Thanawongnuwech, Siti Suri Arshad

et al.

Animals, Journal Year: 2022, Volume and Issue: 12(14), P. 1849 - 1849

Published: July 21, 2022

This paper aims to update the molecular status of porcine circovirus 2 (PCV2) in Malaysia. Firstly, detection rate PCV2 farm and sampled pig population were reported be 83.78% (31/37 farms) 83.54% (66/79 pigs) positive for PCV2, respectively. was detected across all age groups, from fetuses, porkers sows. Co-detection PCV3 antigens also at a 28.77% (21/73). Secondly, antigen Malaysian abattoir lung samples: 18 out 19 (94.74%) samples originating clinically healthy finishers tested positive. Further, this is first study confirm circulation wild boar roaming Peninsular Malaysia, where 28 (100%) found One decade earlier, only genotype PCV2b most recent revealed that genotypes PCV2a, PCV2d present, with being predominant circulating genotype. cap gene nucleotide sequences under negative selection pressure, an estimated substitution 1.102 × 10−3 substitutions/site/year (ssy).

Language: Английский

Citations

13
Development of Duplex LAMP Technique for Detection of Porcine Epidemic Diarrhea Virus (PEDV) and Porcine Circovirus Type 2 (PCV 2) DOI Creative Commons
Supatra Areekit,

Pongbun Tangjitrungrot,

Sintawee Khuchareontaworn

et al.

Current Issues in Molecular Biology, Journal Year: 2022, Volume and Issue: 44(11), P. 5427 - 5439

Published: Nov. 3, 2022

Porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 2 (PCV2) are both important global pathogenic viruses which have a significant impact on the swine industry. In this study, duplex loop-mediated isothermal amplification (duplex LAMP) method was developed in combination with lateral flow dipstick (LFD) for simultaneous detection of PEDV PCV2 using specific sets primers probes designed based conserved regions spike gene (KF272920) an ORF (EF493839), respectively. The limit (LOD) values LAMP-LFD were 0.1 ng/µL 0.246 ng/µL, LOD 10-times more sensitive than conventional PCR RT-PCR-agarose gel-electrophoresis (PCR-AGE RT-PCR-AGE). No cross-reaction to each other that can infect pigs observed according analytical specificity tests. co-infection could be completed within approximately 1.5 h, only simple heating block required amplification. preliminary validation 50 clinical samples positive negative and/or revealed sensitivity, specificity, accuracy all 100% comparison RT-PCR. Hence, study suggests is promising tool early initial screening PCV2, beneficial prevention, planning, epidemiological surveys these diseases.

Language: Английский

Citations

11